@article { , title = {Application of the pMHC array to characterise tumour antigen specific T cell populations in leukaemia patients at disease diagnosis}, abstract = {Immunotherapy treatments for cancer are becoming increasingly successful, however to further improve our understanding of the T-cell recognition involved in effective responses and to encourage moves towards the development of personalised treatments for leukaemia immunotherapy, precise antigenic targets in individual patients have been identified. Cellular arrays using peptide-MHC (pMHC) tetramers allow the simultaneous detection of different antigen specific T-cell populations naturally circulating in patients and normal donors. We have developed the pMHC array to detect CD8+ T-cell populations in leukaemia patients that recognise epitopes within viral antigens (cytomegalovirus (CMV) and influenza (Flu)) and leukaemia antigens (including Per Arnt Sim domain 1 (PASD1), MelanA, Wilms’ Tumour (WT1) and tyrosinase). We show that the pMHC array is at least as sensitive as flow cytometry and has the potential to rapidly identify more than 40 specific T-cell populations in a small sample of T-cells (0.8–1.4 x 106). Fourteen of the twenty-six acute myeloid leukaemia (AML) patients analysed had T cells that recognised tumour antigen epitopes, and eight of these recognised PASD1 epitopes. Other tumour epitopes recognised were MelanA (n = 3), tyrosinase (n = 3) and WT1126-134 (n = 1). One of the seven acute lymphocytic leukaemia (ALL) patients analysed had T cells that recognised the MUC1950-958 epitope. In the future the pMHC array may be used provide point of care T-cell analyses, predict patient response to conventional therapy and direct personalised immunotherapy for patients.}, doi = {10.1371/journal.pone.0140483}, eissn = {1932-6203}, issn = {1932-6203}, issue = {10}, journal = {PLoS ONE}, publicationstatus = {Published}, publisher = {Public Library of Science}, url = {https://hull-repository.worktribe.com/output/1566938}, volume = {10}, keyword = {Health and Health Inequalities}, year = {2015}, author = {Brooks, Suzanne E. and Bonney, Stephanie A. and Lee, Cindy and Publicover, Amy and Khan, Ghazala and Smits, Evelien L. and Sigurdardottir, Dagmar and Arno, Matthew and Li, Demin and Mills, Ken I. and Pulford, Karen and Banham, Alison H. and Van Tendeloo, Viggo and Mufti, Ghulam J. and Rammensee, Hans Georg and Elliott, Tim J. and Orchard, Kim H. and Guinn, Barbara Ann} }