@article { , title = {Toxicity of polyelectrolyte-functionalized titania nanoparticles in zebrafish (Danio rerio) embryos}, abstract = {We investigated the effects of short-term exposure of bare TiO2NPs and polyelectrolyte-coated TiO2NPs in the 5–25nm size range, at relatively high concentrations (of 500 and 1000mg/L) under light or dark conditions, in D. rerio embryos. The biological endpoints investigated included embryo viability and mRNA transcript levels of antioxidant and membrane transport genes relative to control embryos. The presence of nanoparticles on the surface of embryos was assessed using TEM. The results confirm an accumulation of TiO2NPs on the outer surface (chorion) of the embryo, but not within the embryo. No significant difference in embryo viability was detected following each exposure regime. The expression of antioxidant biomarker, SOD2, was significantly impacted by the type of TiO2NP, with TiO2NPs/PSS/PAH coating exposure showing down regulation; the concentration of the nanoparticles, with down regulation at 500mg/L; and dark/light condition with down regulation in the light. The expression levels of the hypoxia and membrane markers, HIF1 and Pxmp2, were not significantly impacted by any factor. The study indicates that SOD2 mRNA expression levels may be useful in the detection of apparent oxidative stress induced by the titania nanoparticle build up on the embryo chorion surface.}, doi = {10.1007/s42452-020-3137-x}, eissn = {2523-3971}, issue = {7}, journal = {SN Applied Sciences}, publicationstatus = {Published}, publisher = {Springer (part of Springer Nature)}, url = {https://hull-repository.worktribe.com/output/3525471}, volume = {2}, keyword = {Specialist Research - Other, Titania nanoparticles, Zebrafsh, Embryos, Oxidative stress}, year = {2020}, author = {Arabeyyat, Zeinab H. and Al-Awady, Mohammed J. and Greenway, Gillian M. and Paunov, Vesselin N. and Rotchell, Jeanette M.} }