@article { , title = {Translocator protein as an imaging marker of macrophage and stromal activation in rheumatoid arthritis pannus}, abstract = {PET radioligands targeted to translocator protein (TSPO) offer a highly sensitive and specific means of imaging joint inflammation in rheumatoid arthritis (RA). Through high expression of TSPO on activated macrophages, TSPO PET has been widely reported in several studies of RA as a means of imaging synovial macrophages in vivo. However, this premise does not take into account the ubiquitous expression of TSPO. This study aimed to investigate TSPO expression in major cellular constituents of RA pannus-monocytes, macrophages, fibroblastlike synoviocytes (FLS cells), and CD4-positive (CD41) T lymphocytes (T cells)-to more accurately interpret TSPO PET signal from RA synovium. Methods: Three RA patients and 3 healthy volunteers underwent PET of both knees using the TSPO radioligand 11 C-PBR28. Through 3 H-PBR28 autoradiography and immunostaining of synovial tissue in 6 RA patients and 6 healthy volunteers, cellular expression of TSPO in synovial tissue was evaluated. TSPO messenger RNA expression and 3 H-PBR28 radioligand binding was assessed using in vitro monocytes, macrophages, FLS cells, and CD41 T cells. Results: 11 C-PBR28 PET signal was significantly higher in RA joints than in healthy joints (average SUV, 0.82 ± 0.12 vs. 0.03 ± 0.004; P , 0.01). Further, 3 H-PBR28-specific binding in synovial tissue was approximately 10-fold higher in RA patients than in healthy controls. Immunofluorescence revealed TSPO expression on macrophages, FLS cells, and CD41 T cells. The in vitro study demonstrated the highest TSPO messenger RNA expression and 3 H-PBR28-specific binding in activated FLS cells, nonactivated M0 macrophages, and activated M2 reparative macrophages, with the least TSPO expression being in activated and nonactivated CD41 T cells. Conclusion: To our knowledge, this study was the first evaluation of cellular TSPO expression in synovium, with the highest TSPO expression and PBR28 binding being found on activated synovial FLS cells and M2 macrophages. TSPO-targeted PET may therefore have a unique sensitivity in detecting FLS cells and macrophage-predominant inflammation in RA, with potential utility for assessing treatment response in trials using novel FLS-cell-targeted therapies.}, doi = {10.2967/jnumed.117.202200}, eissn = {2159-662X}, issn = {0161-5505}, issue = {7}, journal = {Journal of Nuclear Medicine}, pages = {1125-1132}, publicationstatus = {Published}, publisher = {Society of Nuclear Medicine}, url = {https://hull-repository.worktribe.com/output/3630099}, volume = {59}, keyword = {Hull Molecular Imaging Centre, Health and Health Inequalities, Fibroblast-like synoviocytes, Macrophages, Translocator protein, Positron emission tomography}, year = {2018}, author = {Narayan, Nehal and Owen, David R. and Mandhair, Harpreet and Smyth, Erica and Carlucci, Francesco and Saleem, Azeem and Gunn, Roger N. and Rabiner, Eugenii A. and Wells, Lisa and Dakin, Stephanie G. and Sabokbar, Afsie and Taylor, Peter C.} }