@article { , title = {Effect of Particle Wettability and Particle Concentration on the Enzymatic Dehydration of n-Octanaloxime in Pickering Emulsions}, abstract = {© 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH Pickering emulsion systems have emerged as platforms for the synthesis of organic molecules in biphasic biocatalysis. Herein, the catalytic performance was evaluated for biotransformation using whole cells exemplified for the dehydration of n-octanaloxime to n-octanenitrile catalysed by an aldoxime dehydratase (OxdB) overexpressed in E. coli. This study was carried out in Pickering emulsions stabilised solely with silica particles of different hydrophobicity. We correlate, for the first time, the properties of the emulsions with the conversion of the reaction, thus gaining an insight into the impact of the particle wettability and particle concentration. When comparing two emulsions of different type with similar stability and droplet diameter, the oil-in-water (o/w) system displayed a higher conversion than the water-in-oil (w/o) system, despite the conversion in both cases being higher than that in a “classic” two-phase system. Furthermore, an increase in particle concentration prior to emulsification resulted in an increase of the interfacial area and hence a higher conversion.}, doi = {10.1002/anie.202013171}, eissn = {1521-3773}, issn = {1433-7851}, journal = {Angewandte Chemie - International Edition}, publicationstatus = {Published}, publisher = {Wiley}, url = {https://hull-repository.worktribe.com/output/3669941}, volume = {59}, keyword = {Specialist Research - Other, Pickering emulsions, Silica particles, Biphasic catalysis, Enzyme catalysis}, year = {2024}, author = {Bago Rodriguez, Ana Maria and Schober, Lukas and Hinzmann, Alessa and Gröger, Harald and Binks, Bernard P.} }