@article { , title = {The development and evaluation of a conducting matrix for the electrochemical regeneration of the immobilised co-factor NAD(H) under continuous flow}, abstract = {Through the preparation of a novel controlled pore glass-poly(pyrrole) material we have developed a conducting support that is not only suitable for the co-immobilisation of enzymes and co-factors, but also enables the facile electrochemical regeneration of the co-factor during a reaction. Employing the selective reduction of (rac)-2-phenylpropionaldehyde to (S)-phenyl-1-propanol as a model, we have demonstrated the successful co-immobilisation of the HLADH enzyme and co-factor NAD(H); with incorporation of the material into a continuous flow reactor facilitating the in situ electrochemical regeneration of NAD(H) for in excess of 100 h. Using this approach we have developed a reagent-less, atom efficient system applicable to the cost-effective, continuous biosynthesis of chiral compounds.}, doi = {10.1039/b924100k}, eissn = {1477-0539}, issn = {1477-0520}, issue = {10}, journal = {ORGANIC \& BIOMOLECULAR CHEMISTRY}, note = {Output ID 18564.}, pages = {2419-2424}, publicationstatus = {Published}, publisher = {Royal Society of Chemistry}, url = {https://hull-repository.worktribe.com/output/387084}, volume = {8}, keyword = {Specialist Research - Other, Physical and Theoretical Chemistry, Organic Chemistry, Biochemistry}, year = {2010}, author = {Ngamsom, B. and Hickey, A. M. and Greenway, G. M. and Littlechild, J. A. and McCreedy, T. and Watts, P. and Wiles, C.} }