@article { , title = {A cellular screening assay using analysis of metal-modified fluorescence lifetime}, abstract = {Current methods for screening cell receptor internalization often require complex image analysis with limited sensitivity. Here we describe a novel bioassay based on detection of changes in global fluorescence lifetime above a gold substrate, with superresolution axial sensitivity and no need for image analysis. We show that the lifetime of enhanced green fluorescent protein expressed in a cellular membrane is greatly reduced in close proximity to the gold, resulting in a distance-dependent lifetime distribution throughout the cell. We demonstrate the application of this phenomenon in a screening assay by comparing the efficacies of two small molecule inhibitors interfering with the internalization process of a G protein-coupled receptor.}, doi = {10.1016/j.bpj.2010.03.016}, eissn = {1542-0086}, issn = {0006-3495}, issue = {11}, journal = {BIOPHYSICAL JOURNAL}, note = {Output ID 19986.}, pages = {2752-2757}, publicationstatus = {Published}, publisher = {Biophysical Society}, url = {https://hull-repository.worktribe.com/output/387249}, volume = {98}, keyword = {Hull Molecular Imaging Centre, Health and Health Inequalities, CXCR4 antagonist, chemokine receptor, medicinal inorganic, cellular assay, FRET, fluorescence microscopy}, year = {2010}, author = {Cade, Nicholas I. and Fruhwirth, Gilbert and Archibald, Stephen J. and Ng, Tony and Richards, David} }