@article { , title = {Diurnal physiological and immunological responses to a 10-km run in highly trained athletes in an environmentally controlled condition of 6 °C}, abstract = {Purpose The Clara cell protein CC16, secreted from Clara cells in the lung, is discussed as a potential biomarker for toxic effects on the airways. An increased concentration of CC16 in serum may be caused by increased permeability of the lungs. To investigate the changes in P-CC16 in response to an intense exercise bout performed at different times of day (9 am and 4 pm) of highly trained individuals. Method Using a crossover randomized design, 8 runners (mean VO2max 71 ml kg−1 min−1, SD 6) performed a 10-km time trial run, at 9 am and 4 pm, in an environmental chamber set at 6 °C. Lung function tests and blood sampling occurred at baseline, immediately post and 1 h post time trial. Result Diurnal differences (P < 0.05) were found for blood neutrophil and lymphocyte counts; with higher values at 4 pm. P-CC16 was higher at the pre- and post-trial time point at 9 am compared to 4 pm. Lung function was not different between or within trials. Conclusion Morning trial in cold condition caused more physiological strain compared to the same trial in the evening. However, this extra stress caused by zeitgebers could be a useful strategy for athletes, coaches, and general population to improve their running performance and protect their health in cold conditions in the long-term plan.}, doi = {10.1007/s00421-016-3489-5}, eissn = {1439-6327}, issn = {1439-6319}, issue = {1}, journal = {European journal of applied physiology}, pages = {1-6}, publicationstatus = {Published}, publisher = {Springer Verlag}, url = {https://hull-repository.worktribe.com/output/448081}, volume = {117}, keyword = {Health and Health Inequalities, Immunological markers, CC16, Diurnal variation}, year = {2017}, author = {Boukelia, Boukhemis and Fogarty, M. C. and Davison, R. C. R. and Florida-James, G. D.} }