@article { , title = {Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow}, abstract = {An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio) reactions across a rectangular reaction chamber. Functionalised magnetic microparticles are employed as mobile solid-supports and are pulled from one side of the reaction chamber to the other by use of an external magnetic field. As the particles traverse the co-laminar reagent streams, binding and washing steps are performed on their surface in one operation in continuous flow. The applicability of the platform was first demonstrated by performing a proof-of-principle binding assay between streptavidin coated magnetic particles and biotin in free solution with a limit of detection of 20 ng mL(-1) of free biotin. The system was then applied to a mouse IgG sandwich immunoassay as a first example of a process involving two binding steps and two washing steps, all performed within 60 s, a fraction of the time required for conventional testing.}, doi = {10.1039/b904724g}, eissn = {1473-0189}, issn = {1473-0197}, issue = {21}, journal = {Lab On A Chip}, pages = {3110-3117}, publicationstatus = {Published}, publisher = {Royal Society of Chemistry}, url = {https://hull-repository.worktribe.com/output/472879}, volume = {9}, keyword = {Lab on a Chip, Specialist Research - Other, Biochemistry, Bioengineering, General Chemistry, Biomedical Engineering}, year = {2024}, author = {Peyman, Sally A. and Iles, Alexander and Pamme, Nicole} }