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Amplification of bacteria-induced platelet activation is triggered by Fc RIIA, integrin  IIb 3, and platelet factor 4

Arman, M.; Krauel, K.; Tilley, D. O.; Weber, C.; Cox, D.; Greinacher, A.; Kerrigan, S. W.; Watson, S. P.

Authors

M. Arman

K. Krauel

D. O. Tilley

C. Weber

D. Cox

A. Greinacher

S. W. Kerrigan

S. P. Watson



Abstract

Bacterial adhesion to platelets is mediated via a range of strain-specific bacterial surface proteins that bind to a variety of platelet receptors. It is unclear how these interactions lead to platelet activation. We demonstrate a critical role for the immune receptor FcγRIIA, αIIbβ3, and Src and Syk tyrosine kinases in platelet activation by Staphylococcus aureus, Streptococcus sanguinis, Streptococcus gordonii, Streptococcus oralis, and Streptococcus pneumoniae. FcγRIIA activation is dependent on immunoglobulin G (IgG) and αIIbβ3 engagement. Moreover, feedback agonists adenosine 5′-diphosphate and thromboxane A2 are mandatory for platelet aggregation. Additionally, platelet factor 4 (PF4) binds to bacteria and reduces the lag time for aggregation, and gray platelet syndrome α-granule–deficient platelets do not aggregate to 4 of 5 bacterial strains. We propose that FcγRIIA-mediated activation is a common response mechanism used against a wide range of bacteria, and that release of secondary mediators and PF4 serve as a positive feedback mechanism for activation through an IgG-dependent pathway.

Journal Article Type Article
Publication Date May 15, 2014
Journal Blood
Print ISSN 0006-4971
Electronic ISSN 1528-0020
Publisher American Society of Hematology
Peer Reviewed Peer Reviewed
Volume 123
Issue 20
Pages 3166-3174
APA6 Citation Arman, M., Krauel, K., Tilley, D. O., Weber, C., Cox, D., Greinacher, A., …Watson, S. P. (2014). Amplification of bacteria-induced platelet activation is triggered by Fc RIIA, integrin  IIb 3, and platelet factor 4. Blood, 123(20), 3166-3174. https://doi.org/10.1182/blood-2013-11-540526
DOI https://doi.org/10.1182/blood-2013-11-540526
Publisher URL http://www.bloodjournal.org/content/123/20/3166
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