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Endosomes generate localized Rho–ROCK–MLC2–based contractile signals via Endo180 to promote adhesion disassembly

Sturge, Justin; Wienke, Dirk; Isacke, Clare M.

Authors

Dirk Wienke

Clare M. Isacke



Abstract

The regulated assembly and disassembly of focal adhesions and adherens junctions contributes to cell motility and tumor invasion. Pivotal in this process is phosphorylation of myosin light chain-2 (MLC2) by Rho kinase (ROCK) downstream of Rho activation, which generates the contractile force necessary to drive disassembly of epithelial cell–cell junctions and cell–matrix adhesions at the rear of migrating cells. How Rho–ROCK–MLC2 activation occurs at these distinct cellular locations is not known, but the emerging concept that endocytic dynamics can coordinate key intracellular signaling events provides vital clues. We report that endosomes containing the promigratory receptor Endo180 (CD280) can generate Rho–ROCK–MLC2–based contractile signals. Moreover, we provide evidence for a cellular mechanism in which Endo180-containing endosomes are spatially localized to facilitate their contractile signals directly at sites of adhesion turnover. We propose migration driven by Endo180 as a model for the spatial regulation of contractility and adhesion dynamics by endosomes.

Journal Article Type Article
Publication Date Oct 23, 2006
Journal The Journal of Cell Biology
Print ISSN 0021-9525
Electronic ISSN 1540-8140
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 175
Issue 2
Pages 337-347
APA6 Citation Sturge, J., Wienke, D., & Isacke, C. M. (2006). Endosomes generate localized Rho–ROCK–MLC2–based contractile signals via Endo180 to promote adhesion disassembly. Journal of Cell Biology, 175(2), 337-347. https://doi.org/10.1083/jcb.200602125
DOI https://doi.org/10.1083/jcb.200602125
Keywords Cell Biology
Publisher URL http://jcb.rupress.org/content/175/2/337

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