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A feasibility study of a leaky waveguide aptasensor for thrombin

Alamrani, Nasser A.; Greenway, Gillian M.; Pamme, Nicole; Goddard, Nicholas J.; Gupta, Ruchi

Authors

Nasser A. Alamrani

Gillian M. Greenway

Nicholas J. Goddard

Ruchi Gupta



Abstract

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobilised in hydrogels retain their affinity and selectivity towards their target and thus can be used as bioreceptors. The use of antibodies as bioreceptors for sensing thrombin is not viable because it is a serine protease, which will cleave the antibodies. Currently used assays based on clotting time and chromogenic/fluorogenic substrates have limited potential for thrombin measurement in whole blood. Using the initial binding rate over the first 5 min, the limit of detection of our LW aptasensor for thrombin was ∼22 nM. The sensor was tested with spiked serum samples, giving a reading of 46.1 ± 4.6 nM for a sample containing 50 nM thrombin. Our proposed sensor combines the robustness and low cost of aptamers as molecular recognition elements with the simple fabrication process of the chitosan-based leaky waveguide, making LW aptasensors highly attractive for applications in point-of-care diagnostics and healthcare monitoring.

Citation

Alamrani, N. A., Greenway, G. M., Pamme, N., Goddard, N. J., & Gupta, R. (2019). A feasibility study of a leaky waveguide aptasensor for thrombin. Analyst, 144(20), 6048-6054. https://doi.org/10.1039/c9an01421g

Journal Article Type Article
Acceptance Date Sep 6, 2019
Online Publication Date Sep 6, 2019
Publication Date Oct 21, 2019
Deposit Date Sep 22, 2019
Publicly Available Date Sep 7, 2020
Journal Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 144
Issue 20
Pages 6048-6054
DOI https://doi.org/10.1039/c9an01421g
Keywords Analytical Chemistry; Spectroscopy; Electrochemistry; Biochemistry; Environmental Chemistry
Public URL https://hull-repository.worktribe.com/output/2720696
Publisher URL https://pubs.rsc.org/en/content/articlelanding/2019/an/c9an01421g/unauth#!divAbstract
Additional Information : This document is Similarity Check deposited; : Gillian M. Greenway (ORCID); : Nicole Pamme (ORCID); : Nicole Pamme (ResearcherID); : Ruchi Gupta (ORCID); : Ruchi Gupta (ResearcherID); : Single-blind; : Received 27 July 2019; Accepted 6 September 2019; Accepted Manuscript published 6 September 2019; Advance Article published 13 September 2019

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