Eugenia Sanchez
Transcriptomic signatures of experimental alkaloid consumption in a poison frog
Sanchez, Eugenia; Rodríguez, Ariel; Grau, Jose H.; Lötters, Stefan; Künzel, Sven; Saporito, Ralph A.; Ringler, Eva; Schulz, Stefan; Wollenberg Valero, Katharina C.; Vences, Miguel
Authors
Ariel Rodríguez
Jose H. Grau
Stefan Lötters
Sven Künzel
Ralph A. Saporito
Eva Ringler
Stefan Schulz
Katharina C. Wollenberg Valero
Miguel Vences
Abstract
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. In the anuran family Dendrobatidae, aposematic species obtain their toxic or unpalatable alkaloids from dietary sources, a process known as sequestering. To understand how toxicity evolved in this family, it is paramount to elucidate the pathways of alkaloid processing (absorption, metabolism, and sequestering). Here, we used an exploratory skin gene expression experiment in which captive-bred dendrobatids were fed alkaloids. Most of these experiments were performed with Dendrobates tinctorius, but some trials were performed with D. auratus, D. leucomelas and Allobates femoralis to explore whether other dendrobatids would show similar patterns of gene expression. We found a consistent pattern of up-regulation of genes related to muscle and mitochondrial processes, probably due to the lack of mutations related to alkaloid resistance in these species. Considering conserved pathways of drug metabolism in vertebrates, we hypothesize alkaloid degradation is a physiological mechanism of resistance, which was evidenced by a strong upregulation of the immune system in D. tinctorius, and of complement C2 across the four species sampled. Probably related to this strong immune response, we found several skin keratins downregulated, which might be linked to a reduction of the cornified layer of the epidermis. Although not conclusive, our results offer candidate genes and testable hypotheses to elucidate alkaloid processing in poison frogs.
Citation
Sanchez, E., Rodríguez, A., Grau, J. H., Lötters, S., Künzel, S., Saporito, R. A., …Vences, M. (2019). Transcriptomic signatures of experimental alkaloid consumption in a poison frog. Genes, 10(10), 733. https://doi.org/10.3390/genes10100733
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 17, 2019 |
Online Publication Date | Sep 21, 2019 |
Publication Date | 2019-10 |
Deposit Date | Sep 30, 2019 |
Publicly Available Date | Mar 29, 2024 |
Journal | Genes |
Electronic ISSN | 2073-4425 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 10 |
Pages | 733 |
DOI | https://doi.org/10.3390/genes10100733 |
Keywords | Dendrobatidae; Aromobatidae; Dendrobates; Allobates; Epibatidine; Gene expression; Feeding experiment; Immune system; Resistance |
Public URL | https://hull-repository.worktribe.com/output/2786041 |
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Copyright Statement
©2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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