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Loss of CD36 protects against diet-induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis

Verpoorten, Sandrine; Sfyri, Peggy; Scully, David; Mitchell, Robert; Tzimou, Anastasia; Mougios, Vassilis; Patel, Ketan; Matsakas, Antonios

Authors

Sandrine Verpoorten

Peggy Sfyri

David Scully

Robert Mitchell

Anastasia Tzimou

Vassilis Mougios

Ketan Patel



Abstract

Aim: The prevalence of obesity is a major risk factor for cardiovascular and metabolic diseases including impaired skeletal muscle regeneration. Since skeletal muscle regenerative capacity is regulated by satellite cells, we aimed to investigate whether a high-fat diet impairs satellite cell function and whether this is linked to fatty acid uptake via CD36. We also aimed to determine whether loss of CD36 impacts on muscle redox homeostasis and skeletal muscle regenerative capacity.
Methods: We studied the impact of a high-fat diet and CD36 deficiency on murine skeletal muscle morphology, redox homeostasis, satellite cell function, bioenergetics and lipid accumulation in the liver. We also determined the effect of CD36 deficiency on skeletal muscle regeneration.
Results: High-fat diet increased body weight, intramuscular lipid accumulation and oxidative stress in wild-type mice that were significantly mitigated in CD36-deficient mice. High-fat diet and CD36 deficiency independently attenuated satellite cell function on single fibres and myogenic capacity on primary satellite cells. CD36-deficiency resulted in delayed skeletal muscle regeneration following acute injury with cardiotoxin. CD36- deficient and wild-type primary satellite cells had distinct bioenergetic profiles in response to palmitate. High-fat diet induced hepatic steatosis in both genotypes that was more pronounced in the CD36 deficient mice.
Conclusion: This study demonstrates that CD36 deficiency protects against diet-induced obesity, intramuscular lipid deposition and oxidative stress but results in impaired muscle satellite cell function, delayed muscle regeneration and hepatic steatosis. CD36 is a key mediator of fatty acid uptake in skeletal muscle, linking obesity with satellite cell function and muscle regeneration.

Citation

Verpoorten, S., Sfyri, P., Scully, D., Mitchell, R., Tzimou, A., Mougios, V., …Matsakas, A. (2020). Loss of CD36 protects against diet-induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis. Acta Physiologica, 228(3), https://doi.org/10.1111/apha.13395

Journal Article Type Article
Acceptance Date Oct 1, 2019
Online Publication Date Oct 10, 2019
Publication Date Mar 1, 2020
Deposit Date Oct 10, 2019
Publicly Available Date Mar 29, 2024
Journal Acta Physiologica
Print ISSN 1748-1708
Electronic ISSN 1748-1716
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 228
Issue 3
DOI https://doi.org/10.1111/apha.13395
Keywords CD36 deficiency; High‐fat diet; Obesity; Oxidative stress; Regeneration; Satellite cells
Public URL https://hull-repository.worktribe.com/output/2903930
Publisher URL https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.13395
Additional Information Published: 2019-10-10

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Copyright Statement
This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/APHA.13395 All rights reserved






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