Wisanu Thongchai
A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer
Thongchai, Wisanu; Liawruangath, Boonsom; Liawruangrath, Saisunee; Greenway, Gillian M.
Authors
Boonsom Liawruangath
Saisunee Liawruangrath
Gillian M. Greenway
Abstract
A novel chemiluminescence (CL) microfluidic system incorporating a molecularly imprinted polymer (MIP) preconcentration step was used for the determination of chloramphenicol in honey samples. The MIP was prepared by using chloramphenicol as the template, diethylaminoethyl methacrylate (DAM) as the function monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, 2, 2'-dimethoxy-2-phenylacetophenone (DMPA) as the free radical initiator and toluene and dodecanol as the solvent. The MIP was pre-loaded into a 10 mm long, 2 mm wide and 1501,,m deep channel in a planar glass microfluidic device. When the sample containing chloramphenicol was introduced into the microfluidic device it was first preconcentrated on the MIP then detected by an enhancement effect on the chemiluminescence reaction of tris(2, 2'-bipyridyl) ruthenium(II) with cerium(IV) sulphate in sulphuric acid. A micro-syringe pump was used to pump the reagents. The CL intensity was linear in relationship to the chloramphenicol concentrations from 1.55 x 10(-4) to 3.09 x 10(-3) m mol L-1 (r(2) = 0.9915) and the detection limit (3 sigma) and the quantitation limit (10 sigma)were found to be 7.46 x 10(-6) and 2.48 x 10(-5) mu mol L-1, respectively. This method offered a high selectivity and sensitivity for quantitative analysis of chloramphenicol in the honey samples. (C) 2010 Elsevier B.V. All rights reserved.
Citation
Thongchai, W., Liawruangath, B., Liawruangrath, S., & Greenway, G. M. (2010). A microflow chemiluminescence system for determination of chloramphenicol in honey with preconcentration using a molecularly imprinted polymer. Talanta, 82(2), 560-566. https://doi.org/10.1016/j.talanta.2010.05.007
Journal Article Type | Article |
---|---|
Acceptance Date | May 10, 2010 |
Online Publication Date | May 16, 2010 |
Publication Date | Jul 15, 2010 |
Publicly Available Date | Mar 29, 2024 |
Journal | TALANTA |
Print ISSN | 0039-9140 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 82 |
Issue | 2 |
Pages | 560-566 |
DOI | https://doi.org/10.1016/j.talanta.2010.05.007 |
Keywords | Chloramphenicol; Molecularly imprinted polymer (MIP); Chemiluminscence; Microfluidics; MicroFIA |
Public URL | https://hull-repository.worktribe.com/output/396310 |
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