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Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene

Ruiz, Pamela; Orbea, Amaia; Rotchell, Jeanette M.; Cajaraville, Miren P.

Authors

Pamela Ruiz

Amaia Orbea

Miren P. Cajaraville



Abstract

Recent spills in European waters have released polycyclic aromatic hydrocarbons, important components of heavy fuel oil, and the hydrocarbon styrene. Heavy fuel oil and styrene are classified as potentially genotoxic and carcinogenic. Here we investigate transcription of genes involved in cancer development in the liver of juvenile turbots and in the digestive gland of mussels exposed to heavy fuel oil and to styrene and after a recovery period. In turbot, oil produced a significant up-regulation of p53 and gadd45α after 14 days exposure. cyclin G1 was up-regulated after 7 days treatment with styrene. In mussels, ras was downregulated in both treatments after the recovery periods. No mutations in ras hotspots were detected in exposed mussels. gadd45α was up-regulated after the recovery period of the styrene experiment. Overall, transcriptional responses differed in mussels compared to turbot. Turbot responded to hydrocarbon exposure by triggering cell cycle arrest (p53) and DNA repair (gadd45α). © 2012 Springer Science+Business Media, LLC.

Journal Article Type Article
Publication Date 2012-04
Journal ECOTOXICOLOGY
Print ISSN 0963-9292
Electronic ISSN 1573-3017
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 21
Issue 3
Pages 820-831
APA6 Citation Ruiz, P., Orbea, A., Rotchell, J. M., & Cajaraville, M. P. (2012). Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene. Ecotoxicology, 21(3), 820-831. doi:10.1007/s10646-011-0843-6
DOI https://doi.org/10.1007/s10646-011-0843-6
Keywords Heavy fuel oil; Styrene; Turbot; Mussel; Cancer-related genes; Transcriptional responses; Mutation analysis
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