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Directed assembly of yeast cells into living yeastosomes by microbubble templating

Cayre, Olivier J.; Brandy, Marie-Laure; Fakhrullin, Rawil F.; Velev, Orlin D.; Paunov, Vesselin N.

Authors

Olivier J. Cayre

Marie-Laure Brandy

Rawil F. Fakhrullin

Orlin D. Velev

Vesselin N. Paunov V.N.Paunov@hull.ac.uk



Abstract

We report the fabrication of yeastosomes—novel multicellular assemblies, which consist of a spherical monolayer of living yeast cells held together by colloid interactions. Our method is based on templating of microbubbles with cells coated with cationic polyelectrolyte and the layer-by-layer technique. We show the mechanism of formation of yeastosomes which involves a gradual dissolution of the air-cores and their infusion with water. We demonstrate that the cells remain viable in the yeastosome structures. Yeastosomes and similar structures may find applications in the development of novel symbiotic biostructures, artificial multicellular organisms and in tissue engineering. The unusual structure of these multicellular assemblies resembles the primitive forms of multicellular species, like Volvox, and other algae and could be regarded as one possible stage of the evolutionary development of multicellularity.

Citation

Brandy, M., Cayre, O. J., Fakhrullin, R. F., Velev, O. D., & Paunov, V. N. (2010). Directed assembly of yeast cells into living yeastosomes by microbubble templating. Soft matter, 6(15), 3494-3498. https://doi.org/10.1039/c0sm00003e

Journal Article Type Article
Acceptance Date Apr 1, 2010
Online Publication Date May 17, 2010
Publication Date Aug 7, 2010
Journal SOFT MATTER
Print ISSN 1744-683X
Electronic ISSN 1744-6848
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 6
Issue 15
Pages 3494-3498
DOI https://doi.org/10.1039/c0sm00003e
Keywords Yeastosomes; Microbubbles; Directed assembly; Polyelectrolytes; Multicellularity; Templating; Cellosomes
Public URL https://hull-repository.worktribe.com/output/424049