Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis

Ngamsom, Bongkot; Esfahani, Mohammad M.N.; Phurimsak, Chayakom; Lopez-Martinez, Maria J.; Raymond, Jean-Claude; Broyer, Patrick; Patel, Pradip; Pamme, Nicole

Abstract

This study reports multiplex sorting of Salmonella typhimurium and Escherichia coli 0157, from broth cultures and from pathogen-spiked skinned chicken breast enrichment broths by employing microfluidic free-flow magnetophoresis. Magnetic beads of different sizes and magnetite content, namely Dynabeads anti-salmonella and Hyglos-Streptavidin beads together with the corresponding pathogen-specific biotinylated recombinant phages, were utilised as affinity solid phases for the capture and concentration of viable S. typhimurium and E. coli 0157. Following optimisation, the protocol was used to demonstrate continuous magnetophoretic sorting of the two pathogen-bound magnetic bead populations from mixed cultures and from pathogen-spiked chicken pre-enrichment broths under the influence of a Halbach magnet array. For example, in the la tter case, a pure population of S. typhimurium-bound Dynabeads (72% recovery) was sorted from a 100 μL mixture containing E. coli 0157-bound Hyglos beads (67% recovery) within 1.2 min in the presence of 0.1% Tween 20. This proof-of-principle study demonstrates how more than one pathogen type can be simultaneously isolated/enriched from a single food pre-enrichment broth (e.g. Universal food enrichment broth).

Citation

Ngamsom, B., Esfahani, M. M., Phurimsak, C., Lopez-Martinez, M. J., Raymond, J., Broyer, P., …Pamme, N. (2016). Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis. Analytica Chimica Acta, 918, 69-76. https://doi.org/10.1016/j.aca.2016.03.014

• Journal Article Type: Article
• Acceptance Date: Mar 7, 2016
• Online Publication Date: Mar 18, 2016
• Publication Date: Apr 28, 2016
• Deposit Date: Mar 22, 2016
• Publicly Available Date: Mar 22, 2016
• Journal: Analytica chimica acta
• Print ISSN: 0003-2670
• Electronic ISSN: 1873-4324
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 918
• Pages: 69-76
• DOI: https://doi.org/10.1016/j.aca.2016.03.014
• Keywords: Multiplex sorting, Salmonella & E.coli 0157, Food enrichment broth, Free-flow magnetophoresis, Affinity magnetic separation
• Public URL: https://hull-repository.worktribe.com/output/434001
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0003267016303312
• Additional Information: This article is maintained by: Elsevier; Article Title: Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis; Journal Title: Analytica Chimica Acta; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.aca.2016.03.014; Content Type: article; Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.