Janine Wäge
Short-term effects of CO₂-induced low pH exposure on target gene expression in Platynereis dumerilii
Wäge, Janine; Rohr, Silvia; Hardege, Jorg D; Rotchell, Jeanette M
Authors
Silvia Rohr
Jorg D Hardege
Professor Jeanette Rotchell J.Rotchell@hull.ac.uk
Professor and Associate Dean for Research and Enterprise
Abstract
Objective: Increasing atmospheric CO₂ concentration are causing changes to the seawater carbonate chemistry, lowering the pH and we study potential impacts of these changes at the molecular level in a non-calcifying, marine polychaete species Platynereis dumerilii. Methods: we investigate the relative expression of carbonic anhydrase (CA), Na+/H+ exchangers (NHE), and calmodulin (CaM) genes from P. dumerilii under acidified seawater conditions (pH 7.8) induced by CO₂ using qPCR. Results: mRNA expression of CA in the CO₂-induced worms was significantly up-regulated at low pH conditions (pH 7.8, 1h), suggesting changes in acid-base balance. In contrast, the expression of NHE and CaM showed no significant change. In addition, we compare these results to a previous study using inorganic acid (HCl)-induced pH changes. Conclusions: results suggest that carbonate chemistry has an impact on gene expression that differs from pH-associated change. To our knowledge, this is the first study that compares low pH exposure experiments using HCl and CO₂ as the inducing agents.
Citation
Wäge, J., Rohr, S., Hardege, J. D., & Rotchell, J. M. (2016). Short-term effects of CO₂-induced low pH exposure on target gene expression in Platynereis dumerilii. Journal of Marine Biology and Oceanography, 5(2), https://doi.org/10.4172/2324-8661.1000155
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 29, 2016 |
Online Publication Date | Jul 5, 2016 |
Publication Date | Jul 5, 2016 |
Deposit Date | Jul 8, 2016 |
Publicly Available Date | Jul 8, 2016 |
Journal | Journal of marine biology and oceanography |
Print ISSN | 2324-8661 |
Publisher | OMICS International |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Issue | 2 |
DOI | https://doi.org/10.4172/2324-8661.1000155 |
Keywords | Ocean acidification, CO₂, Gene expression, Polychaeta, P. dumerilii, pH |
Public URL | https://hull-repository.worktribe.com/output/440721 |
Publisher URL | http://www.scitechnol.com/peer-review/shortterm-effects-of-co2induced-low-ph-exposure-on-target-gene-expression-in-platynereis-dumerilii-uX0c.php?article_id=5276 |
Additional Information | This is a description of an article which has been published in: Journal of marine biology and oceanography, 2016, v.5, issue 2. |
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©2016 University of Hull
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