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Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles

Stratton, Dan; Moore, Colin; Zheng, Lei; Lange, Sigrun; Inal, Jameel

Authors

Colin Moore

Lei Zheng

Sigrun Lange

Jameel Inal



Abstract

© 2015 Elsevier Inc.All rights reserved. MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMs-1over 11 s, reaching a peak [Ca2+]iof 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMs-1over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at ∼430 nM.

Journal Article Type Article
Publication Date May 8, 2015
Journal Biochemical and Biophysical Research Communications
Print ISSN 0006-291X
Electronic ISSN 1090-2104
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 460
Issue 3
Pages 511-517
APA6 Citation Stratton, D., Moore, C., Zheng, L., Lange, S., & Inal, J. (2015). Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles. Biochemical and biophysical research communications, 460(3), 511-517. doi:10.1016/j.bbrc.2015.03.061
DOI https://doi.org/10.1016/j.bbrc.2015.03.061
Keywords Microvesicles; Refractory period; Sublytic C5b-9; Intravesicular calcium
Publisher URL https://www.sciencedirect.com/science/article/pii/S0006291X15005070?via%3Dihub
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