Maja Marinovic
Assaying Rho GTPase–dependent processes in Dictyostelium discoideum
Marinovic, Maja; Xiong, Huajiang; Rivero Crespo, Francisco; Weber, Igor
Authors
Huajiang Xiong
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Igor Weber
Abstract
The model organism D. discoideum is well-suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion and development. D. discoideum cells make use of Rho-regulated signaling pathways to reorganize the actin cytoskeleton during chemotaxis, endocytosis and cytokinesis. In this organism the Rho family encompasses 20 members, several belonging to the Rac subfamily, but there are no representatives of the Cdc42 and Rho subfamilies. Here we present protocols suitable for monitoring the actin polymerization response and the activation of Rac upon stimulation of aggregation competent cells with the chemoattractant cAMP, and for monitoring the localization and dynamics of Rac activity in live cells.
Citation
Marinovic, M., Xiong, H., Rivero Crespo, F., & Weber, I. (2018). Assaying Rho GTPase–dependent processes in Dictyostelium discoideum. Methods in molecular biology, 1821, 371-392. https://doi.org/10.1007/978-1-4939-8612-5_25
Journal Article Type | Article |
---|---|
Acceptance Date | May 11, 2018 |
Online Publication Date | Jul 31, 2018 |
Publication Date | Jul 31, 2018 |
Deposit Date | Jul 11, 2018 |
Publicly Available Date | Mar 29, 2024 |
Print ISSN | 1064-3745 |
Peer Reviewed | Peer Reviewed |
Volume | 1821 |
Pages | 371-392 |
DOI | https://doi.org/10.1007/978-1-4939-8612-5_25 |
Keywords | Actin polymerization; cAMP; Chemotaxis; Dictyostelium; Fluorescent probes; FRET; GTPase-binding domain; Pull-down assay, Rac |
Public URL | https://hull-repository.worktribe.com/output/923295 |
Publisher URL | https://link.springer.com/protocol/10.1007%2F978-1-4939-8612-5_25 |
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©2018 The University of Hull
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