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Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake (2019)
Journal Article
Lawson Handley, L., Read, D. S., Winfield, I. J., Kimbell, H., Johnson, H., Li, J., …Hänfling, B. (2019). Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake. Environmental DNA, 1(1), 26-39. https://doi.org/10.1002/edn3.5

Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repea... Read More

Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds (2018)
Journal Article
Harper, L. R., Buxton, A. S., Rees, H. C., Bruce, K., Brys, R., Halfmaerten, D., …Hänfling, B. (2019). Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds. Hydrobiologia, 826(1), 25-41. doi:10.1007/s10750-018-3750-5

© 2018, The Author(s). Environmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity monitoring tool with enormous potential to inform aquatic conservation and management. Development is ongoing, with strong commercial inter... Read More

Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types (2018)
Journal Article
Sellers, G. S., Di Muri, C., Gómez, A., & Hänfling, B. (2018). Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types. Metabarcoding and Metagenomics, 2, (e24556). doi:10.3897/mbmg.2.24556. ISSN 2534-9708

Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of hi... Read More

The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding (2018)
Journal Article
Li, J., Lawson Handley, L. J., Read, D. S., & Hänfling, B. (2018). The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding. Molecular Ecology Resources, 18(5), 1102-1114. doi:10.1111/1755-0998.12899

© 2018 John Wiley & Sons Ltd. Environmental DNA (eDNA) is a promising tool for rapid and noninvasive biodiversity monitoring. eDNA density is low in environmental samples, and a capture method, such as filtration, is often required to concentrate e... Read More

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Gough, K. C., …Hänfling, B. (2018). Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus). Ecology and Evolution, 8(12), (6330-6341). doi:10.1002/ece3.4013. ISSN 2045-7758

Environmental DNA (eDNA) analysis is a rapid, cost‐effective, non‐invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species‐specific survey tool for rar... Read More

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive (2018)
Journal Article
Hering, D., Borja, A., Jones, J. I., Pont, D., Boets, P., Bouchez, A., …Kelly, M. (2018). Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive. Water Research, 138, (192-205). doi:10.1016/j.watres.2018.03.003. ISSN 0043-1354

Assessment of ecological status for the European Water Framework Directive (WFD) is based on “Biological Quality Elements” (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is... Read More

A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations (2017)
Journal Article
Sellers, G. S., Griffin, L. R., Hänfling, B., & Gómez, A. (2017). A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations. PeerJ, 2017(5), doi:10.7717/peerj.3228

© 2017 Sellers et al. The tadpole shrimp, Triops cancriformis, is a freshwater crustacean listed as endangered in the UK and Europe living in ephemeral pools. Populations are threatened by habitat destruction due to land development for agriculture a... Read More

Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK (2017)
Journal Article
Blackman, R. C., Constable, D., Hahn, C., Sheard, A. M., Durkota, J., Hänfling, B., & Handley, L. L. (2017). Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK. Aquatic Invasions, 12(2), (177-189). doi:10.3391/ai.2017.12.2.06. ISSN 1798-6540

© 2017 The Author(s). and 2017 REABIC. We report the discovery of a non-native gammarid, Gammarus fossarum (Koch, 1836) (Crustacea, Amphipoda), in UK rivers. Gammarus fossarum is a common freshwater gammarid in many parts of mainland Europe, but was... Read More

Genetic evidence challenges the native status of a threatened freshwater fish (Carassius carassius) in England (2017)
Journal Article
Jeffries, D. L., Copp, G. H., Maes, G. E., Lawson Handley, L., Sayer, C. D., & Hänfling, B. (2017). Genetic evidence challenges the native status of a threatened freshwater fish (Carassius carassius) in England. Ecology and Evolution, 7(9), (2871-2882). doi:10.1002/ece3.2831. ISSN 2045-7758

© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. A fundamental consideration for the conservation of a species is the extent of its native range, that is, regions naturally colonized. However, both natural processes and... Read More

Environmental DNA metabarcoding of lake fish communities reflects long-term data from established survey methods (2016)
Journal Article
Hänfling, B., Lawson Handley, L., Read, D. S., Hahn, C., Li, J., Nichols, P., …Winfield, I. J. (2016). Environmental DNA metabarcoding of lake fish communities reflects long-term data from established survey methods. Molecular ecology, 25(13), 3101-3119. https://doi.org/10.1111/mec.13660

Organisms continuously release DNA into their environments via shed cells, excreta, gametes and decaying material. Analysis of this ‘environmental DNA’ (eDNA) is revolutionizing biodiversity monitoring. eDNA outperforms many established survey method... Read More

Comparing RADseq and microsatellites to infer complex phylogeographic patterns, an empirical perspective in the Crucian carp, Carassius carassius, L. (2016)
Journal Article
Jeffries, D. L., Copp, G. H., Lawson Handley, L., Olsén, K. H., Sayer, C. D., & Hänfling, B. (2016). Comparing RADseq and microsatellites to infer complex phylogeographic patterns, an empirical perspective in the Crucian carp, Carassius carassius, L. Molecular ecology, 25(13), 2997-3018. https://doi.org/10.1111/mec.13613

The conservation of threatened species must be underpinned by phylogeographic knowledge. This need is epitomized by the freshwater fish Carassius carassius, which is in decline across much of its European range. Restriction site-associated DNA sequen... Read More

The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545 (2015)
Journal Article
Hutchinson, W. F., Culling, M., Orton, D. C., Hänfling, B., Handley, L. L., Hamilton-Dyer, S., …Barrett, J. H. (2015). The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545. Royal Society Open Science, 2(9), (150199). doi:10.1098/rsos.150199. ISSN 2054-5703

© 2015 The Authors. A comparison of ancient DNA (single-nucleotide polymorphisms) and carbon and nitrogen stable isotope evidence suggests that stored cod provisions recovered from the wreck of the Tudor warship Mary Rose, which sank in the Solent, s... Read More

Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis (2015)
Journal Article
Mathers, T. C., Hammond, R. L., Jenner, R., Hänfling, B., Atkins, J., & Gómez, A. (2015). Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity, 115(1), 37-46. doi:10.1038/hdy.2015.10

© 2015 Macmillan Publishers Limited All rights reserved. Transitions in sexual system and reproductive mode may affect the course of sex chromosome evolution, for instance by altering the strength of sexually antagonistic selection. However, there ha... Read More

Multiple global radiations in tadpole shrimps challenge the concept of 'living fossils' (2013)
Journal Article
Mathers, T. C., Hammond, R. L., Jenner, R. A., Hänfling, B., & Gomez, A. (2013). Multiple global radiations in tadpole shrimps challenge the concept of 'living fossils'. PeerJ, 1, e62. doi:10.7717/peerj.62

‘Living fossils’, a phrase first coined by Darwin, are defined as species with limited recent diversification and high morphological stasis over long periods of evolutionary time. Morphological stasis, however, can potentially lead to div... Read More

High lability of sexual system over 250 million years of evolution in morphologically conservative tadpole shrimps (2013)
Journal Article
Mathers, T. C., Jenner, R. A., Zierold, T., Hammond, R. L., Hänfling, B., & Gomez, A. (2013). High lability of sexual system over 250 million years of evolution in morphologically conservative tadpole shrimps. BMC evolutionary biology, 13(1), (30). doi:10.1186/1471-2148-13-30. ISSN 1471-2148

Background Sexual system is a key factor affecting the genetic diversity, population structure, genome structure and the evolutionary potential of species. The sexual system androdioecy – where males and hermaphrodites coexist in populations – is ext... Read More

Invasive alien Crustacea: dispersal, establishment, impact and control (2011)
Journal Article
Hänfling, B., Edwards, F., & Gherardi, F. (2011). Invasive alien Crustacea: dispersal, establishment, impact and control. BioControl, 56(4), (573-595). doi:10.1007/s10526-011-9380-8. ISSN 1386-6141

The subphylum Crustacea includes the most successful species among aquatic alien invaders. The impacts of invasive alien crustaceans (IAC) are often substantial, due to the complex trophic role of most of these species leading to cascading effects th... Read More

Invasive cyprinid fish in Europe originate from the single introduction of an admixed source population followed by a complex pattern of spread (2011)
Journal Article
Volckaert, F. A. M., Britton, R., Gozlan, R., Hänfling, B., Simon, A., van Oosterhout, C., & Volckaert, F. A. M. (2011). Invasive cyprinid fish in Europe originate from the single introduction of an admixed source population followed by a complex pattern of spread. PloS one, 6(6), doi:10.1371/journal.pone.0018560

The Asian cyprinid fish, the topmouth gudgeon (Pseudorasbora parva), was introduced into Europe in the 1960s. A highly invasive freshwater fish, it is currently found in at least 32 countries outside its native range. Here we analyse a 700 base pair... Read More

Shallow phylogeographic structuring of Vimba vimba across Europe suggests two distinct refugia during the last glaciation (2009)
Journal Article
Duempelmann, C., Braendle, M., Hänfling, B., Bogutskaya, N. G., Brandl, R., Brändle, M., & Dümpelmann, C. (2009). Shallow phylogeographic structuring of Vimba vimba across Europe suggests two distinct refugia during the last glaciation. Journal of fish biology, 75(9), (2269-2286). doi:10.1111/j.1095-8649.2009.02415.x. ISSN 0022-1112

Genetic variation and geographical structuring of vimba Vimba vimba were analysed across 26 sites (80 individuals) by means of mtDNA sequences (cyt b gene, mitochondrial control region) to localize hypothesized glacial refugia and to reconstruct post... Read More

Elevated mtDNA diversity in introduced populations of Cynotilapia afra(Günther 1894) in Lake Malawi National Park is evidence for multiple source populations and hybridization (2009)
Journal Article
HÄNFLING, B., van OOSTERHOUT, C., TURNER, G. F., ZIDANA, H., Van Oosterhout, C., & Haenfling, B. (2009). Elevated mtDNA diversity in introduced populations of Cynotilapia afra(Günther 1894) in Lake Malawi National Park is evidence for multiple source populations and hybridization. Molecular ecology, 18(21), 4380-4389. doi:10.1111/j.1365-294x.2009.04362.x

Sex ratio, reproductive mode and genetic diversity in Triops cancriformis (2009)
Journal Article
Zierold, T., Montero-Pau, J., Hänfling, B., & Gómez, A. (2009). Sex ratio, reproductive mode and genetic diversity in Triops cancriformis. Freshwater biology, 54(7), 1392-1405. doi:10.1111/j.1365-2427.2009.02191.x

1. Aquatic invertebrates display a wide array of alternative reproductive modes from apomixis to hermaphroditism and cyclical parthenogenesis. These have important effects on genetic diversity and population structure. Populations of the 'living foss... Read More