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A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow (2016)
Journal Article
Dawson, A., Dyer, C., Macfie, J., Davies, J., Karsai, L., Greenman, J., & Jacobsen, M. (2016). A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow. Biomicrofluidics, 10(6), (064101). doi:10.1063/1.4964813. ISSN 1932-1058

© 2016 Author(s). The study of inflammatory bowel disease, including Ulcerative Colitis and Crohn's Disease, has relied largely upon the use of animal or cell culture models; neither of which can represent all aspects of the human pathophysiology. Pr... Read More

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), (3188-3195). doi:10.1002/elps.201100172. ISSN 0173-0835

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More

A microfluidic device for tissue biopsy culture and interrogation (2010)
Journal Article
Webster, A., Haswell, S. J., Dyer, C. E., & Greenman, J. (2010). A microfluidic device for tissue biopsy culture and interrogation. Analytical Methods, 2(8), 1005-1007. doi:10.1039/c0ay00293c

This communication reports the development of a microfluidic device capable of maintaining the long-term culture of viable tissue biopsies. Tissue-based models will enable evaluation of cell-cell and cell-matrix interactions within multi-cellular sys... Read More

Detection of nitric oxide in tissue samples by ESI-MS (2009)
Journal Article
Shen, Z., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., & Haswell, S. J. (2010). Detection of nitric oxide in tissue samples by ESI-MS. The Analyst, 135(2), (302-305). doi:10.1039/b917643h. ISSN 0003-2654

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chose... Read More

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), (231-233). doi:10.1016/j.aca.2009.03.038. ISSN 0003-2670

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More

Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device (2009)
Journal Article
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. doi:10.1039/b913309g

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was... Read More

Development of a microfluidic device for the maintenance and interrogation of viable tissue biopsies (2008)
Journal Article
Dyer, C. E., Greenman, J., Haswell, S. J., & Hattersley, S. M. (2008). Development of a microfluidic device for the maintenance and interrogation of viable tissue biopsies. Lab on a chip, 8(11), (1842-1846). doi:10.1039/b809345h. ISSN 1473-0197

A microfluidic based experimental methodology has been developed that offers a biomimetic microenvironment in which pseudo in vivo tissue studies can be carried out under in vitro conditions. Using this innovative technique, which utilizes the inhere... Read More