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Harvesting human prostate tissue material and culturing primary prostate epithelial cells (2016)
Book Chapter
Frame, F. M., Pellacani, D., Collins, A. T., & Maitland, N. J. (2016). Harvesting human prostate tissue material and culturing primary prostate epithelial cells. In I. J. McEwan (Ed.), The nuclear receptor superfamily: methods and protocols, 181-201. Humana Press. https://doi.org/10.1007/978-1-4939-3724-0_12

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell l... Read More

Cancer Stem Cells, Models of Study and Implications of Therapy Resistance Mechanisms (2011)
Book Chapter
Frame, F. M., & Maitland, N. J. (2011). Cancer Stem Cells, Models of Study and Implications of Therapy Resistance Mechanisms. In J. S. Rhim, & R. Kremer (Eds.), Human cell transformation, 105-118. Springer. https://doi.org/10.1007/978-1-4614-0254-1_9

There is now compelling evidence for tumour initiating or cancer stem cells (CSCs) in human cancers. The current evidence of this CSC hypothesis, the CSC phenotype and methods of identification, culture and in vitro modelling will be presented, with... Read More