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Coronin 1 Is Required for Integrin β2 Translocation in Platelets (2020)
Journal Article
Riley, D. R. J., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (in press). Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International Journal of Molecular Sciences, 21(1), https://doi.org/10.3390/ijms21010356

Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins i... Read More about Coronin 1 Is Required for Integrin β2 Translocation in Platelets.

Biochemical and immunocytochemical characterization of coronins in platelets (2019)
Journal Article
Riley, D. R., Khalil, J. S., Naseem, K. M., & Rivero, F. (in press). Biochemical and immunocytochemical characterization of coronins in platelets. Platelets, 1-12. https://doi.org/10.1080/09537104.2019.1696457

Results Platelets express at least four coronins Proteomics and transcriptomics studies indicate that both human and mouse platelets express Coro1, 2, 3 and 7, while other coronins are practically undetectable (Supplemental Table 1 and 2). To demonst... Read More about Biochemical and immunocytochemical characterization of coronins in platelets.

Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3 (2019)
Journal Article
Berger, M., Riley, D. R. J., Lutz, J., Khalil, J. S., Aburima, A., Naseem, K. M., & Rivero, F. (2019). Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3. Cells, 8(2), 149. https://doi.org/10.3390/cells8020149

Typical Rho GTPases, like Rac1, Cdc42 and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB su... Read More about Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3.

The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart (2019)
Journal Article
Onwuli, D. O., Samuel, S., Sfyri, P., Welham, K., Goddard, M., Abu-Omar, Y., …Beltran-Alvarez, P. (2019). The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. International journal of cardiology, 282, 76-80. https://doi.org/10.1016/j.ijcard.2019.01.102

Background The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-tra... Read More about The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart.

Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line (2018)
Journal Article
Samuel, S. F., Marsden, A. J., Deepak, S., Rivero, F., Greenman, J., & Beltran-Alvarez, P. (2018). Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line. Proteomes, 6(4), https://doi.org/10.3390/proteomes6040044

Glioblastomas (GBM) are the most common grade 4 brain tumours; patients have very poor prognosis with an average survival of 15 months after diagnosis. Novel research lines have begun to explore aberrant protein arginine methylation (ArgMe) as a poss... Read More about Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line.

Assaying Rho GTPase–dependent processes in Dictyostelium discoideum (2018)
Journal Article
Marinovic, M., Xiong, H., Rivero Crespo, F., & Weber, I. (2018). Assaying Rho GTPase–dependent processes in Dictyostelium discoideum. Methods in molecular biology, 1821, 371-392. doi:10.1007/978-1-4939-8612-5_25

The model organism D. discoideum is well-suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion and develo... Read More about Assaying Rho GTPase–dependent processes in Dictyostelium discoideum.

Methods to study the roles of Rho GTPases in platelet function (2018)
Journal Article
Rivero Crespo, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. Methods in molecular biology, 1821, 199-217. doi:10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

Assaying Rho GTPase-dependent processes in Dictyostelium discoideum (2018)
Journal Article
Marinović, M., Xiong, H., Rivero, F., & Weber, I. (2018). Assaying Rho GTPase-dependent processes in Dictyostelium discoideum. Methods in Molecular Biology, 1821, 371-392. doi:10.1007/978-1-4939-8612-5_25

The model organism D. discoideum is well-suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion and develo... Read More about Assaying Rho GTPase-dependent processes in Dictyostelium discoideum.

Methods to study the roles of Rho GTPases in platelet function (2018)
Book Chapter
Rivero, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. In Methods in Molecular Biology; Rho GTPases, 199-217. Humana Press. doi:10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8(1), https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation.

The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8, https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation.

Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid (2016)
Journal Article
Krey, J. F., Krystofiak, E. S., Dumont, R. A., Vijayakumar, S., Choi, D., Rivero, F., …Barr-Gillespie, P. G. (2016). Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. Journal of Cell Biology, 215(4), 467-482 . https://doi.org/10.1083/jcb.201606036

With their essential role in inner-ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by crosslinkers of the plastin, fascin, and espin families. While mi... Read More about Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.

Atypical Rho GTPases of the RhoBTB Subfamily: Roles in vesicle trafficking and tumorigenesis (2016)
Journal Article
Ji, W., & Rivero, F. (2016). Atypical Rho GTPases of the RhoBTB Subfamily: Roles in vesicle trafficking and tumorigenesis. Cells, 5(2), 28. https://doi.org/10.3390/cells5020028

RhoBTB proteins constitute a subfamily of atypical Rho GTPases represented in mammals by RhoBTB1, RhoBTB2, and RhoBTB3. Their characteristic feature is a carboxyl terminal extension that harbors two BTB domains capable of assembling cullin 3-dependen... Read More about Atypical Rho GTPases of the RhoBTB Subfamily: Roles in vesicle trafficking and tumorigenesis.

RHOBTB3 (Rho-related BTB domain containing 3) (2016)
Journal Article
Cai, S., & Rivero, F. (2016). RHOBTB3 (Rho-related BTB domain containing 3). Atlas of genetics and cytogenetics in oncology and haematology, 20(12), doi:10.4267/2042/66947

RHOBTB3 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. In RHOBTB3 the GTPase doma... Read More about RHOBTB3 (Rho-related BTB domain containing 3).

RHOBTB2 (Rho-related BTB domain containing 2) (2016)
Journal Article
Cai, S., Rivero-Crespo, F., & Schenková, K. (2016). RHOBTB2 (Rho-related BTB domain containing 2). Atlas of genetics and cytogenetics in oncology and haematology,

RhoBTB2 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. RHOBTB2 is a putative tumo... Read More about RHOBTB2 (Rho-related BTB domain containing 2).

RHOBTB1 (Rho-related BTB domain containing 1) (2016)
Journal Article
Cai, S., Rivero-Crespo, F., & Schenková, K. (2016). RHOBTB1 (Rho-related BTB domain containing 1). Atlas of genetics and cytogenetics in oncology and haematology, 20(10), 512-515. https://doi.org/10.4267/2042/66084

RhoBTB1 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. RHOBTB1 is a putative tumo... Read More about RHOBTB1 (Rho-related BTB domain containing 1).

Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum (2011)
Journal Article
Rivero Crespo, F. (2011). Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum. Genome Biology, 12(2), https://doi.org/10.1186/gb-2011-12-2-r20

Background: The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk cells. There are four groups of... Read More about Comparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum.

Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication (2011)
Journal Article
Heidel, A. J., Lawal, H. M., Felder, M., Schilde, C., Helps, N. R., Tunggal, B., …Glöckner, G. (2011). Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication. Genome research, 21(11), 1882-1891. doi:10.1101/gr.121137.111

Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms. To understand genome evolution over long ti... Read More about Phylogeny-wide analysis of social amoeba genomes highlights ancient origins for complex intercellular communication.