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Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis (2012)
Journal Article
Cubero-Leon, E., Minier, C., Hill, E. M., & Rotchell, J. (2012). Metabolomic analysis of sex specific metabolites in gonads of the mussel, Mytilus edulis. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 7(2), (212-219). doi:10.1016/j.cbd.2012.03.002. ISSN 1744-117X

Marine mussels have been used as sentinel organisms to monitor exposure to a variety of chemical contaminants, including endocrine disrupting chemicals, in the aquatic environment. Although they are an important species for use in ecotoxicology inves... Read More

Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene (2012)
Journal Article
Ruiz, P., Orbea, A., Rotchell, J. M., & Cajaraville, M. P. (2012). Transcriptional responses of cancer-related genes in turbot Scophthalmus maximus and mussels Mytilus edulis exposed to heavy fuel oil no. 6 and styrene. Ecotoxicology, 21(3), 820-831. doi:10.1007/s10646-011-0843-6

Recent spills in European waters have released polycyclic aromatic hydrocarbons, important components of heavy fuel oil, and the hydrocarbon styrene. Heavy fuel oil and styrene are classified as potentially genotoxic and carcinogenic. Here we investi... Read More

Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure (2012)
Journal Article
Cubero-Leon, E., Ciocan, C. M., Minier, C., & Rotchell, J. M. (2012). Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure. Environmental science and pollution research international, 19(7), (2728-2733). doi:10.1007/s11356-012-0772-9. ISSN 0944-1344

Purpose: The aim of this study is to develop a normalization method for real-time PCR data by analyzing the most stably expressed control genes in mussel (Mytilus edulis) reproductive tissue. Methods: To facilitate this, six candidate genes, includin... Read More

Analytical challenges and the development of biomarkers to measure and to monitor the effects of ocean acidification (2011)
Journal Article
Hardege, J., Rotchell, J., Terschak, J., & Greenway, G. (2011). Analytical challenges and the development of biomarkers to measure and to monitor the effects of ocean acidification. Trends in Analytical Chemistry, 30(8), 1320-1326. https://doi.org/10.1016/j.trac.2011.07.004

Changing ocean-carbonate chemistry caused by oceanic uptake of anthropogenic atmospheric carbon dioxide leads to the formation of carbonic acid, thus lowering the pH of the sea with predictions of a decrease from current levels at 8.15 to 7.82 by the... Read More

Identification of Reproduction-Specific genes associated with maturation and estrogen exposure in a marine bivalve Mytilus edulis (2011)
Journal Article
Ciocan, C. M., Cubero-Leon, E., Minier, C., & Rotchell, J. M. (2011). Identification of Reproduction-Specific genes associated with maturation and estrogen exposure in a marine bivalve Mytilus edulis. PloS one, 6(7), e22326. https://doi.org/10.1371/journal.pone.0022326

Background: While it is established that vertebrate-like steroids, particularly estrogens (estradiol, estrone) and androgens (testosterone), are present in various tissues of molluscs, it is still unclear what role these play in reproductive endocrin... Read More

A new in vitro screening bioassay for the ecotoxicological evaluation of the estrogenic responses of environmental chemicals using roach (Rutilus rutilus) liver explant culture (2010)
Journal Article
Gerbron, M., Geraudie, P., Rotchell, J., & Minier, C. (2010). A new in vitro screening bioassay for the ecotoxicological evaluation of the estrogenic responses of environmental chemicals using roach (Rutilus rutilus) liver explant culture. Environmental Toxicology, 25(5), 510-516. doi:10.1002/tox.20596

There is growing evidence that many chemicals released in the environment are able to disturb the normal endocrinology of organisms affecting the structure and function of their reproductive system. This has prompted the scientific community to devel... Read More

Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis (2010)
Journal Article
Ciocan, C. M., Cubero-Leon, E., Puinean, A. M., Hill, E. M., Minier, C., Osada, M., …Rotchell, J. M. (2010). Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis. Environmental pollution, 158(9), (2977-2984). doi:10.1016/j.envpol.2010.05.025. ISSN 0269-7491

Mytilus edulis were exposed to 17β-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2)... Read More

Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis) (2010)
Journal Article
Cubero-Leon, E., Ciocan, C. M., Hill, E. M., Osada, M., Kishida, M., Itoh, N., …Rotchell, J. M. (2010). Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquatic Toxicology, 98(2), (178-187). doi:10.1016/j.aquatox.2010.02.007. ISSN 0166-445X

Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in... Read More

Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination (2009)
Journal Article
Nogueira, P., Pacheco, M., Lourdes Pereira, M., Mendo, S., & Rotchell, J. M. (2010). Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. Environmental pollution, 158(5), (1783-1790). doi:10.1016/j.envpol.2009.11.007. ISSN 0269-7491

The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from... Read More

Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene (2009)
Journal Article
Nogueira, P., Lourenço, J., Rodriguez, E., Pacheco, M., Santos, C., Rotchell, J. M., & Mendo, S. (2009). Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene. Aquatic Toxicology, 94(2), (123-130). doi:10.1016/j.aquatox.2009.06.007. ISSN 0166-445X

The molecular responses induced during and after an acute exposure to 7,12-dimethylbenz[a]anthracene (DMBA) were analysed in liver, gill and blood cells of juvenile Anguilla anguilla with the aim of developing molecular biomarkers of environmental PA... Read More

Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen (2009)
Journal Article
Denier, X., Hill, E. M., Rotchell, J., & Minier, C. (2009). Estrogenic activity of cadmium, copper and zinc in the yeast estrogen screen. Toxicology in Vitro, 23(4), (569-573). doi:10.1016/j.tiv.2009.01.006. ISSN 0887-2333

Heavy metals are increasingly studied due to their apparent ability to disrupt signaling pathways of living organisms including humans. Among various mechanisms of action, metals are suspected of exerting estrogenic activity in human and wildlife. In... Read More

A novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans (2008)
Journal Article
Rotchell, J. M., du Corbier, F. A., Stentiford, G. D., Lyons, B. P., Liddle, A. R., & Ostrander, G. K. (2009). A novel population health approach: using fish retinoblastoma gene profiles as a surrogate for humans. Comparative Biochemistry and Physiology - Part C: Toxicology and Pharmacology, 149(2), (134-140). doi:10.1016/j.cbpc.2008.09.009. ISSN 1532-0456

Retinoblastoma, a tumor suppressor gene, is frequently mutated in diverse types of human tumors. We have previously shown that two types of fish tumor, eye and liver, also possess mutant Rb genes. Our aim is to determine if the Rb allele status is li... Read More

Ras gene in marine mussels: A molecular level response to petrochemical exposure (2008)
Journal Article
Lima, I., Peck, M. R., Rendón-Von Osten, J., Soares, A. M. V. M., Guilhermino, L., & Rotchell, J. (2008). Ras gene in marine mussels: A molecular level response to petrochemical exposure. Marine pollution bulletin, 56(4), (633-640). doi:10.1016/j.marpolbul.2008.01.018. ISSN 0025-326X

Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the... Read More

To what extent are hepatic concentrations of heavy metals in Anguilla anguilla at a site in a contaminated estuary related to body size and age and reflected in the metallothionein concentrations? (2007)
Journal Article
Bird, D. J., Rotchell, J. M., Hesp, S. A., Newton, L. C., Hall, N. G., & Potter, I. C. (2008). To what extent are hepatic concentrations of heavy metals in Anguilla anguilla at a site in a contaminated estuary related to body size and age and reflected in the metallothionein concentrations?. Environmental pollution, 151(3), (641-651). doi:10.1016/j.envpol.2007.03.018. ISSN 0269-7491

We explored how hepatic [metal]s in Anguilla anguilla at a contaminated estuarine site are influenced by body size, age and season, and the extent that [Cu], [Cd] and [Zn]s are reflected in [metallothionein (MT)]s. Although each [metal] and [MT] incr... Read More

Conservation of cancer genes in the marine invertebrate Mytilus edulis (2005)
Journal Article
Ciocan, C. M., & Rotchell, J. M. (2005). Conservation of cancer genes in the marine invertebrate Mytilus edulis. Environmental Science and Technology, 39(9), (3029-3033). doi:10.1021/es0400887. ISSN 0013-936X

Mussels are susceptible to a wide range of environmental toxicants, including carcinogens, and thus are often employed as bioindicator species. To elucidate the molecular aetiology of such neoplastic damage, we have cloned Mytilus edulis homologues o... Read More