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The prognostic significance of serum interferon-gamma (IFN-γ) in hormonally dependent breast cancer (2022)
Journal Article
Todorović-Raković, N., Milovanović, J., Greenman, J., & Radulovic, M. (2022). The prognostic significance of serum interferon-gamma (IFN-γ) in hormonally dependent breast cancer. Cytokine, 152, Article 155836. https://doi.org/10.1016/j.cyto.2022.155836

Background: Interferon-γ (IFN-γ) is a pleiotropic immunomodulatory cytokine. Because of its contradictory and even dualistic roles in malignancies, its potential as a biomarker remains to be unraveled. Aim: To evaluate the prognostic significance of... Read More about The prognostic significance of serum interferon-gamma (IFN-γ) in hormonally dependent breast cancer.

Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device (2021)
Journal Article
Foster, H., Wade, M., England, J., Greenman, J., & Green, V. (2021). Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device. Organs-on-a-Chip, 3, Article 100011. https://doi.org/10.1016/j.ooc.2021.100011

Abstract This report demonstrates the ability of a microfluidic device to maintain human Graves' disease tissue enabling the isolation and characterisation of Graves' disease specific exosomes. Graves' disease (n = 7) and non-Graves’ disease (Hashim... Read More about Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device.

Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device (2021)
Journal Article
Riley, A., Jones, H., England, J., Kuvshinov, D., Green, V., & Greenman, J. (2021). Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device. Oncology Letters, 22(5), Article 780. https://doi.org/10.3892/ol.2021.13041

Although a large cohort of potential biomarkers for thyroid cancer aggressiveness have been tested in various formats in recent years, to the best of our knowledge, thyro‑ globulin and calcitonin remain the only two established biomarkers associated... Read More about Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device.

De-palmitoylation of tissue factor regulates its activity, phosphorylation and cellular functions (2021)
Journal Article
Rondon, A. M., Ettelaie, C., Featherby, S., Rondon, A. M. R., Greenman, J., Versteeg, H. H., & Maraveyas, A. (2021). De-palmitoylation of tissue factor regulates its activity, phosphorylation and cellular functions. Cancers, 13(15), https://doi.org/10.3390/cancers13153837

In this study, the role of de-palmitoylation of tissue factor (TF) in the decryption of its activity was explored. TF-tGFP constructs were prepared by mutagenesis-substitution at Cys245 to prevent or mimic palmitolyation. Additionally, to reduce TF d... Read More about De-palmitoylation of tissue factor regulates its activity, phosphorylation and cellular functions.

Factor VIIa regulates the level of cell-surface tissue factor through separate but cooperative mechanisms (2021)
Journal Article
Madkhali, Y., Rondon, A. M., Featherby, S., Maraveyas, A., Greenman, J., & Ettelaie, C. (2021). Factor VIIa regulates the level of cell-surface tissue factor through separate but cooperative mechanisms. Cancers, 13(15), https://doi.org/10.3390/cancers13153718

Procoagulant activity of tissue factor (TF) in response to injury or inflammation is accompanied with cellular signals which determine the fate of cells. However, to prevent excessive signalling, TF is rapidly dissipated through release into microves... Read More about Factor VIIa regulates the level of cell-surface tissue factor through separate but cooperative mechanisms.

The significance of HOXB7 and IL17RB serum levels in prognosis of hormonally dependent breast cancer: A pilot study (2021)
Journal Article
Todorović-Raković, N., Milovanović, J., Greenman, J., & Radulovic, M. (2021). The significance of HOXB7 and IL17RB serum levels in prognosis of hormonally dependent breast cancer: A pilot study. Advances in Medical Sciences, 66(2), 359-365. https://doi.org/10.1016/j.advms.2021.07.007

Purpose: Improved prognostication of a patient's outcome could allow for personalized treatment decisions in breast cancer. Homeobox B7 (HOXB7) and interleukin 17 receptor B (IL17RB) are proteins reportedly involved in the development of hormonal the... Read More about The significance of HOXB7 and IL17RB serum levels in prognosis of hormonally dependent breast cancer: A pilot study.

Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review (2021)
Journal Article
Jones, H., Green, V., England, J., & Greenman, J. (2021). Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review. Future Science OA, 7(7), https://doi.org/10.2144/fsoa-2021-0041

Thyroid cancer incidence and related mortality is increasing year-on-year, and although treatment for early disease with surgery and radioiodine results in a 98% 5-year survival rate, recurrence and treatment refractory disease is evident in an unacc... Read More about Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review.

Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices (2021)
Journal Article
Christensen, M. G., Cawthorne, C., Dyer, C. E., Greenman, J., & Pamme, N. (2021). Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices. Microfluidics and Nanofluidics, 25(4), https://doi.org/10.1007/s10404-021-02434-x

Microfluidic ‘organ-on-a-chip’ devices hold great potential for better mimicking the continuous flow microenvironment experienced by tissue and cells in vivo, thereby ensuring realistic transport of nutrients and elimination of waste products. Howeve... Read More about Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices.

Arginine methylation: the promise of a ‘silver bullet’ for brain tumours? (2021)
Journal Article
Samuel, S. F., Barry, A., Greenman, J., & Beltran-Alvarez, P. (in press). Arginine methylation: the promise of a ‘silver bullet’ for brain tumours?. Amino acids, https://doi.org/10.1007/s00726-020-02937-x

Despite intense research efforts, our pharmaceutical repertoire against high-grade brain tumours has not been able to increase patient survival for a decade and life expectancy remains at less than 16 months after diagnosis, on average. Inhibitors of... Read More about Arginine methylation: the promise of a ‘silver bullet’ for brain tumours?.

Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities (2020)
Journal Article
Mohammad, M. A., Greenman, J., Maraveyas, A., & Ettelaie, C. (2020). Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities. Scientific reports, 10(1), https://doi.org/10.1038/s41598-020-77963-6

Tissue factor (TF) signalling has been associated with alterations in Akt activity influencing cellular survival and proliferation. TF is also shown to induce signalling through activation of the protease activated receptor (PAR)2. Seven cell lines w... Read More about Activation of PAR2 by tissue factor induces the release of the PTEN from MAGI proteins and regulates PTEN and Akt activities.

Doxorubicin Enhances Procoagulant Activity of Endothelial Cells after Exposure to Tumour Microparticles on Microfluidic Devices (2020)
Journal Article
Algarni, A., Greenman, J., & Madden, L. A. (2020). Doxorubicin Enhances Procoagulant Activity of Endothelial Cells after Exposure to Tumour Microparticles on Microfluidic Devices. Bloods, 1(1), 23-34. https://doi.org/10.3390/bloods1010006

The majority of cancer patients undergoing chemotherapy have a significantly increased risk of venous thromboembolism via a mechanism not yet fully elucidated but which most probably involves tumour microparticles (MP) combined with damaged/activated... Read More about Doxorubicin Enhances Procoagulant Activity of Endothelial Cells after Exposure to Tumour Microparticles on Microfluidic Devices.

The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review (2020)
Journal Article
Chew, D., Green, V., Riley, A., England, R. J., & Greenman, J. (2020). The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review. Frontiers in Surgery, 7, https://doi.org/10.3389/fsurg.2020.00043

© Copyright © 2020 Chew, Green, Riley, England and Greenman. Background: Thyroid cancer is the most common endocrine malignancy worldwide. Primary treatment with surgery and radioactive iodine is usually successful, however, there remains a small pro... Read More about The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review.

Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow (2019)
Journal Article
Algarni, A., Greenman, J., & Madden, L. A. (2019). Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow. Biomicrofluidics, 13(6), https://doi.org/10.1063/1.5123462

Tumor patients are at a high risk of venous thromboembolism (VTE), and the mechanism by which this occurs may involve tumor-derived microvesicles (MVs). Previously, it has been shown that tumor MVs become attached to endothelial cells in static condi... Read More about Procoagulant tumor microvesicles attach to endothelial cells on biochips under microfluidic flow.

Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model? (2019)
Journal Article
Olubajo, F., Achawal, S., & Greenman, J. (2020). Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?. Translational Oncology, 13(1), 1-10. https://doi.org/10.1016/j.tranon.2019.09.002

© 2019 The Authors BACKGROUND: One way to overcome the genetic and molecular variations within glioblastoma is to treat each tumour on an individual basis. To facilitate this, we have developed a microfluidic culture paradigm that maintains human gli... Read More about Development of a Microfluidic Culture Paradigm for Ex Vivo Maintenance of Human Glioblastoma Tissue: A New Glioblastoma Model?.

Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling (2019)
Journal Article
Ethaeb, A. M., Mohammad, M. A., Madkhali, Y., Maraveyas, A., Featherby, S., Greenman, J., & Ettelaie, C. (2020). Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling. Apoptosis, 25(1-2), 29-41. https://doi.org/10.1007/s10495-019-01576-2

Accumulation of tissue factor (TF) within cells leads to cellular apoptosis mediated through p38 and p53 pathways. In this study, the involvement of Src1 in the induction of TF-mediated cell apoptosis, and the mechanisms of Src1 activation were inves... Read More about Accumulation of tissue factor in endothelial cells promotes cellular apoptosis through over-activation of Src1 and involves β1-integrin signalling.

The effect of radioiodine treatment on the diseased thyroid gland (2019)
Journal Article
Riley, A. S., McKenzie, G. A. G., Green, V., Schettino, G., England, R. J. A., & Greenman, J. (2019). The effect of radioiodine treatment on the diseased thyroid gland. International Journal of Radiation Biology, 95(12), 1718-1727. https://doi.org/10.1080/09553002.2019.1665206

Purpose: Radioiodine (I131) therapy is the treatment mainstay for several benign and malignant thyroid disorders, however I131 is known to cause DNA damage and liberation of thyroidal self-antigens inducing secondary immunoreactivity. The exact mecha... Read More about The effect of radioiodine treatment on the diseased thyroid gland.

Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research (2019)
Journal Article
Soultanidis, G., Subiel, A., Renard, I., Reinhart, A. M., Green, V. L., Oelfke, U., …Cawthorne, C. J. (2019). Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research. Physics in Medicine and Biology, 64(12), https://doi.org/10.1088/1361-6560/ab215b

Significant improvements in radiotherapy are likely to come from biological rather than technical optimization, for example increasing tumour radiosensitivity via combination with targeted therapies. Such paradigms must first be evaluated in preclini... Read More about Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research.

The Ratio of Factor VIIa:Tissue Factor Content within Microvesicles Determines the Differential Influence on Endothelial Cells (2019)
Journal Article
Madkhali, Y., Featherby, S., Collier, M., Maraveyas, A., Greenman, J., & Ettelaie, C. (2019). The Ratio of Factor VIIa:Tissue Factor Content within Microvesicles Determines the Differential Influence on Endothelial Cells. TH Open, 03(02), e132-e145. https://doi.org/10.1055/s-0039-1688934

Tissue factor (TF)-positive microvesicles from various sources can promote cellular proliferation or alternatively induce apoptosis, but the determining factors are unknown. In this study the hypothesis that the ratio of fVIIa:TF within microvesicles... Read More about The Ratio of Factor VIIa:Tissue Factor Content within Microvesicles Determines the Differential Influence on Endothelial Cells.

A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens (2019)
Journal Article
Kennedy, R., Kuvshinov, D., Sdrolia, A., Kuvshinova, E., Hilton, K., Crank, S., …Greenman, J. (2019). A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens. Scientific reports, 9(1), https://doi.org/10.1038/s41598-019-42745-2

Development of personalised cancer models to predict response to radiation would benefit patient care; particularly in malignancies where treatment resistance is prevalent. Herein, a robust, easy to use, tumour-on-a-chip platform which maintains prec... Read More about A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens.

Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms (2019)
Journal Article
Featherby, S., Xiao, Y. P., Ettelaie, C., Nikitenko, L. L., Greenman, J., & Maraveyas, A. (2019). Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms. Scientific reports, 9(1), https://doi.org/10.1038/s41598-019-42738-1

The bidirectional association between coagulation and cancer has been established. However, anticoagulant therapies have been reported to have beneficial outcomes by influencing the vascularisation of the tumours. In this study the influence of a set... Read More about Low molecular weight heparin and direct oral anticoagulants influence tumour formation, growth, invasion and vascularisation by separate mechanisms.