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The toxic effect of cytostatics on primary cilia frequency and multiciliation (2019)
Journal Article
Filipová, A., Diaz Garcia, D., Bezrouk, A., Čížková, D., Dvořák, J., Filip, S., …Šinkorová, Z. (2019). The toxic effect of cytostatics on primary cilia frequency and multiciliation. Journal of Cellular and Molecular Medicine, 23(8), 5728-5736. https://doi.org/10.1111/jcmm.14487

The primary cilium is considered as a key component of morphological cellular stability. However, cancer cells are notorious for lacking primary cilia in most cases, depending upon the tumour type. Previous reports have shown the effect of starvation... Read More about The toxic effect of cytostatics on primary cilia frequency and multiciliation.

Synthesis of super bright indium phosphide colloidal quantum dots through thermal diffusion (2019)
Journal Article
Clarke, M. T., Viscomi, F. N., Chamberlain, T. W., Hondow, N., Adawi, A. M., Sturge, J., …Stasiuk, G. J. (2019). Synthesis of super bright indium phosphide colloidal quantum dots through thermal diffusion. Communications Chemistry, 2(1), https://doi.org/10.1038/s42004-019-0138-z

Indium phosphide based quantum dots have emerged in recent years as alternatives to traditional heavy metal (cadmium, lead) based materials suitable for biomedical application due to their non-toxic nature. The major barrier to this application, is t... Read More about Synthesis of super bright indium phosphide colloidal quantum dots through thermal diffusion.

A 18F radiolabelled Zn(ii) sensing fluorescent probe (2018)
Journal Article
Price, T. W., Firth, G., Eling, C. J., Kinnon, M., Long, N. J., Sturge, J., & Stasiuk, G. J. (2018). A 18F radiolabelled Zn(ii) sensing fluorescent probe. Chemical communications : Chem comm / the Royal Society of Chemistry, 54(26), 3227-3230 . https://doi.org/10.1039/c8cc00687c

A selective fluorescent probe for Zn(ii), AQA-F, has been synthesized. AQA-F exhibits a ratiometric shift in emission of up to 80 nm upon binding Zn(ii) ([AQA-F] = 0.1 mM, [Zn(ii)Cl 2 ] = 0-300 μM). An enhancement of quantum yield from Φ = 4.2% to Φ... Read More about A 18F radiolabelled Zn(ii) sensing fluorescent probe.

How to study basement membrane stiffness as a biophysical trigger in prostate cancer and other age-related pathologies or metabolic diseases (2016)
Journal Article
Rodriguez-Teja, M., Breit, C., Clarke, M., Talar, K., Wang, K., Mohammad, M. A., …Sturge, J. (2016). How to study basement membrane stiffness as a biophysical trigger in prostate cancer and other age-related pathologies or metabolic diseases. Journal of visualized experiments : JoVE, 2016(115), https://doi.org/10.3791/54230

Here we describe a protocol that can be used to study the biophysical microenvironment related to increased thickness and stiffness of the basement membrane (BM) during age-related pathologies and metabolic disorders (e.g. cancer, diabetes, microvasc... Read More about How to study basement membrane stiffness as a biophysical trigger in prostate cancer and other age-related pathologies or metabolic diseases.

Endo180 at the cutting edge of bone cancer treatment and beyond (2016)
Journal Article
Sturge, J. (2016). Endo180 at the cutting edge of bone cancer treatment and beyond. Journal of Pathology, 238(4), 485-488. https://doi.org/10.1002/path.4673

Skeletal bone is an attractive site for secondary tumour growth and is also home to spontaneous primary cancer. Treatment of bone metastasis is focused on limiting the vicious cycle of bone destruction with bisphosphonates or inhibition of receptor a... Read More about Endo180 at the cutting edge of bone cancer treatment and beyond.

Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces (2015)
Journal Article
Caley, M. P., King, H., Shah, N., Wang, K., Rodriguez-Teja, M., Gronau, J. H., …Sturge, J. (2016). Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces. Clinical & experimental metastasis, 33(2), 151-165. https://doi.org/10.1007/s10585-015-9765-7

The diverse composition and structure of extracellular matrix (ECM) interfaces encountered by tumor cells at secondary tissue sites can influence metastatic progression. Extensive in vitro and in vivo data has confirmed that metastasizing tumor cells... Read More about Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces.

AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival (2014)
Journal Article
Rodriguez-Teja, M., Gronau, J. H., Breit, C., Zhang, Y. Z., Minamidate, A., Caley, M. P., …Sturge, J. (2015). AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. Journal of Pathology, 235(4), 581-592. https://doi.org/10.1002/path.4485

Biomechanical strain imposed by age-related thickening of the basal lamina and augmented tissue stiffness in the prostate gland coincides with increased cancer risk. Here we hypothesized that the structural alterations in the basal lamina associated... Read More about AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival.

LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression (2014)
Journal Article
Mura, M., Hopkins, T. G., Michael, T., Abd-Latip, N., Weir, J., Aboagye, E., …Blagden, S. P. (2015). LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression. Oncogene, 34(39), 5025-5036. https://doi.org/10.1038/onc.2014.428

RNA-binding proteins (RBPs) bind to and post-transcriptionally regulate the stability of mRNAs. La-related protein 1 (LARP1) is a conserved RBP that interacts with poly-A-binding protein and is known to regulate 5′-terminal oligopyrimidine tract (TOP... Read More about LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression.

Survival outcome and EMT suppression mediated by a lectin domain interaction of Endo180 and CD147 (2014)
Journal Article
Rodriguez-Teja, M., Gronau, J. H., Minamidate, A., Darby, S., Gaughan, L., Robson, C., …Sturge, J. (2015). Survival outcome and EMT suppression mediated by a lectin domain interaction of Endo180 and CD147. Molecular cancer research, 13(3), 538-547. https://doi.org/10.1158/1541-7786.mcr-14-0344-t

Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin do... Read More about Survival outcome and EMT suppression mediated by a lectin domain interaction of Endo180 and CD147.

Corrigendum (2013)
Journal Article
Waxman, J., Cobb, J. P., Palmieri, C., Rhim, J. S., Pchejetski, D., Mauri, F., …Sturge, J. (2013). Corrigendum. Journal of Pathology, 229(3), e4-e4. https://doi.org/10.1002/path.4156

Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer (2012)
Journal Article
Palmieri, C., Caley, M. P., Purshouse, K., Fonseca, A., Rodriguez-Teja, M., Kogianni, G., …Sturge, J. (2013). Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer. The British journal of cancer / Cancer Research Campaign, 108(1), 163-169. https://doi.org/10.1038/bjc.2012.540

Background:Endo180 (CD280; MRC2; uPARAP)-dependent collagen remodelling is dysregulated in primary tumours and bone metastasis. Here, we confirm the release and diagnostic accuracy of soluble Endo180 for diagnosing metastasis in breast cancer (BCa).M... Read More about Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer.

Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells (2012)
Journal Article
Ramasamy, K., Khatun, H., Macpherson, L., Caley, M. P., Sturge, J., Mufti, G. J., …Calle, Y. (2012). Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells. British journal of haematology, 157(5), 564-579. doi:10.1111/j.1365-2141.2012.09103.x

The response of the tumour microenvironment to anti-cancer drugs can influence treatment efficacy. Current drug-screening methodologies fail to distinguish and quantify simultaneously the concomitant effect of drugs on the tumour stroma and cancer ce... Read More about Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells.

Circulating sphingosine-1-phosphate and erythrocyte sphingosine kinase-1 activity as novel biomarkers for early prostate cancer detection (2012)
Journal Article
Nunes, J., Naymark, M., Sauer, L., Muhammad, A., Keun, H., Sturge, J., …Pchejetski, D. (2012). Circulating sphingosine-1-phosphate and erythrocyte sphingosine kinase-1 activity as novel biomarkers for early prostate cancer detection. The British journal of cancer / Cancer Research Campaign, 106(5), (909-915). doi:10.1038/bjc.2012.14. ISSN 0007-0920

Background: Current markers available for screening normal populations and for monitoring prostate cancer (PCa) treatment lack sensitivity and selectivity. Sphingosine-1-phosphate (S1P) is a circulating lipid second messenger involved in cell growth... Read More about Circulating sphingosine-1-phosphate and erythrocyte sphingosine kinase-1 activity as novel biomarkers for early prostate cancer detection.

TGFβ1-Endo180-dependent collagen deposition is dysregulated at the tumour-stromal interface in bone metastasis (2011)
Journal Article
Caley, M. P., Kogianni, G., Adamarek, A., Gronau, J. H., Rodriguez-Teja, M., Fonseca, A., …Sturge, J. (2012). TGFβ1-Endo180-dependent collagen deposition is dysregulated at the tumour-stromal interface in bone metastasis. Journal of Pathology, 226(5), 775-783. doi:10.1002/path.3958

Cellular niches in adult tissue can harbour dysregulated microenvironments that become the driving force behind disease progression. The major environmental change when metastatic cells arrive in the bone is the destruction of mineralized type I coll... Read More about TGFβ1-Endo180-dependent collagen deposition is dysregulated at the tumour-stromal interface in bone metastasis.

Bone metastasis in prostate cancer: Emerging therapeutic strategies (2011)
Journal Article
Sturge, J., Caley, M. P., & Waxman, J. (2011). Bone metastasis in prostate cancer: Emerging therapeutic strategies. Nature Reviews Clinical Oncology, 8(6), (357-368). doi:10.1038/nrclinonc.2011.67. ISSN 1759-4774

Metastatic bone disease (MBD) in advanced-stage cancer increases the risk of intractable bone pain, pathological skeletal fracture, spinal-cord compression and decreased survival. The disease manifestation course during MBD is largely driven by homot... Read More about Bone metastasis in prostate cancer: Emerging therapeutic strategies.

Biological and clinical implications of nicastrin expression in invasive breast cancer (2010)
Journal Article
Filipović, A., Gronau, J. H., Green, A. R., Wang, J., Vallath, S., Shao, D., …Coombes, R. C. (2010). Biological and clinical implications of nicastrin expression in invasive breast cancer. Breast cancer research and treatment, 125(1), 43-53. doi:10.1007/s10549-010-0823-1

Nicastrin is an essential component of the gamma secretase (GS) enzyme complex, required for its synthesis and recognition of substrates for proteolytic cleavage. The purpose of this study was to investigate whether nicastrin has prognostic value or... Read More about Biological and clinical implications of nicastrin expression in invasive breast cancer.