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Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair (2021)
Journal Article
Wilkinson, H. N., Kidd, A. S., Roberts, E. R., & Hardman, M. J. (2021). Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair. Journal of visualized experiments : JoVE, 168, https://doi.org/10.3791/62326

© 2021 JoVE Creative Commons. Chronic non-healing wounds, which primarily affect the elderly and diabetic, are a significant area of clinical unmet need. Unfortunately, current chronic wound treatments are inadequate, while available pre-clinical mod... Read More about Human ex vivo wound model and whole-mount staining approach to accurately evaluate skin repair.

Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model (2020)
Journal Article
Wilkinson, H. N., Longhorne, F. L., Roberts, E. R., Brownhill, V. R., & Hardman, M. J. (in press). Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model. Wound Repair and Regeneration, https://doi.org/10.1111/wrr.12888

Negative pressure wound therapy is a widely used treatment for chronic, nonhealing wounds. Surprisingly, few studies have systematically evaluated the cellular and molecular effects of negative pressure treatment on human skin. In addition, no study... Read More about Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model.

Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria (2020)
Journal Article
Weldrick, P. J., Hardman, M. J., & Paunov, V. N. (2021). Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria. Materials Chemistry Frontiers, 5(2), 961-972. https://doi.org/10.1039/d0qm00874e

Treating bacterial infections with species demonstrating antibiotic resistance to the chosen antibiotic is often hindered due to the ability of certain bacteria to grow biofilms where they can effectively hide and resist the antibiotic action. We rep... Read More about Smart active antibiotic nanocarriers with protease surface functionality can overcome biofilms of resistant bacteria.

Wound healing: cellular mechanisms and pathological outcomes (2020)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2020). Wound healing: cellular mechanisms and pathological outcomes. Open Biology, 10(9), https://doi.org/10.1098/rsob.200223

Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular behaviours, as occurs with diabetes and ageing, can lead... Read More about Wound healing: cellular mechanisms and pathological outcomes.

Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds (2020)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2020). Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds. Frontiers in Cell and Developmental Biology, 8, https://doi.org/10.3389/fcell.2020.00773

© Copyright © 2020 Wilkinson and Hardman. Cellular senescence is a fundamental stress response that restrains tumour formation. Yet, senescence cells are also present in non-cancerous states, accumulating exponentially with chronological age and cont... Read More about Senescence in Wound Repair: Emerging Strategies to Target Chronic Healing Wounds.

Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing (2020)
Journal Article
Brownhill, V., Huddleston, E., Bell, A., Hart, J., Webster, I., Hardman, M., & Wilkinson, H. (2021). Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing. Advances in wound care, 10(7), 345-356. https://doi.org/10.1089/wound.2020.1218

Objective: Traditional negative pressure wound therapy systems can be large and cumbersome, limiting patient mobility and adversely affecting quality of life. PICO™, a no canister single-use system offers a lightweight, portable alternative to tradit... Read More about Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing.

Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers (2019)
Journal Article
Weldrick, P. J., Hardman, M. J., & Paunov, V. N. (2019). Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers. ACS Applied Materials & Interfaces, 11(47), 43902-43919. https://doi.org/10.1021/acsami.9b16119

© 2019 American Chemical Society. Biofilms are prevalent in chronic wounds and once formed are very hard to remove, which is associated with poor outcomes and high mortality rates. Biofilms are comprised of surface-attached bacteria embedded in an ex... Read More about Enhanced Clearing of Wound-Related Pathogenic Bacterial Biofilms Using Protease-Functionalized Antibiotic Nanocarriers.

Optimising platelet secretomes to deliver robust tissue-specific regeneration (2019)
Journal Article
Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., …Matsakas, A. (2020). Optimising platelet secretomes to deliver robust tissue-specific regeneration. Journal of tissue engineering and regenerative medicine, 14(1), 82-98. https://doi.org/10.1002/term.2965

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet-based applications promote cell division and can be customised for tissue-specific efficacy, this makes them strong candidates for developing novel reg... Read More about Optimising platelet secretomes to deliver robust tissue-specific regeneration.

Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22 (2019)
Journal Article
Wilkinson, H. N., Roberts, E. R., Stafford, A. R., Banyard, K. L., Matteucci, P., Mace, K. A., & Hardman, M. J. (2019). Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22. American Journal of Pathology, 189(11), 2196-2208. https://doi.org/10.1016/j.ajpath.2019.07.015

Macrophages are important for effective iron recycling and erythropoiesis, but they also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds and a requireme... Read More about Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22.

Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling (2019)
Journal Article
Wilkinson, H. N., Upson, S. E., Banyard, K. L., Knight, R., Mace, K. A., & Hardman, M. J. (2019). Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling. Journal of Investigative Dermatology, 139(11), 2368-2377.e7. https://doi.org/10.1016/j.jid.2019.05.014

Iron is crucial for maintaining normal bodily function with well-documented roles in erythropoiesis, hemostasis, and inflammation. Despite this, little is known about the temporal regulation of iron during wound healing, or how iron contributes to wo... Read More about Reduced Iron in Diabetic Wounds: An Oxidative Stress-Dependent Role for STEAP3 in Extracellular Matrix Deposition and Remodeling.

Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality (2019)
Journal Article
Weldrick, P. J., Iveson, S., Hardman, M. J., & Paunov, V. N. (2019). Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality. Nanoscale, 11(21), 10472-10485. https://doi.org/10.1039/c8nr10022e

Multidrug-resistant pathogens are prevalent in chronic wounds. There is an urgent need to develop novel antimicrobials and formulation strategies that can overcome antibiotic resistance and provide a safe alternative to traditional antibiotics. This... Read More about Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality.

Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. (2019)
Journal Article
Wilkinson, H. N., Clowes, C., Banyard, K. L., Matteuci, P., Mace, K., & Hardman, M. J. (2019). Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. Journal of Investigative Dermatology, 139(5), 1171-1181.e6. https://doi.org/10.1016/j.jid.2019.01.005

© 2019 The Authors Cellular senescence can be broadly defined as a stable, but essentially irreversible, loss of proliferative capacity. Historically, senescence has been described as a negative outcome of advanced cellular age. It is now clear, howe... Read More about Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2..

Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers (2018)
Journal Article
Al-Awady, M. J., Weldrick, P. J., Hardman, M. J., Greenway, G. M., & Paunov, V. N. (2018). Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers. Materials Chemistry Frontiers, 2(11), 2032-2044. https://doi.org/10.1039/c8qm00343b

We have developed and tested a novel functionalised nanocarrier for chlorhexidine (CHX) which provides a very strong enhancement of its antimicrobial action. The nanocarrier was based on lightly-cross-linked acrylate copolymer nanogel particles loade... Read More about Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers.

A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model (2018)
Journal Article
Wilkinson, H. N., Iveson, S., Catherall, P., & Hardman, M. J. (2018). A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model. Frontiers in Microbiology, 9(JUL), https://doi.org/10.3389/fmicb.2018.01450

Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic steward... Read More about A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model.

Microbial host interactions and impaired wound healing in mice and humans: defining a role for BD14 and NOD2 (2018)
Journal Article
Williams, H., Campbell, L., Crompton, R. A., Singh, G., McHugh, B. J., Davidson, D. J., …Hardman, M. J. (2018). Microbial host interactions and impaired wound healing in mice and humans: defining a role for BD14 and NOD2. Journal of Investigative Dermatology, 138(10), 2264-2274. https://doi.org/10.1016/j.jid.2018.04.014

Chronic wounds cause significant patient morbidity and mortality. A key factor in their etiology is microbial infection, yet skin host-microbiota interactions during wound repair remain poorly understood. Microbiome profiles of non-infected human chr... Read More about Microbial host interactions and impaired wound healing in mice and humans: defining a role for BD14 and NOD2.

The role of estrogen in cutaneous ageing and repair (2017)
Journal Article
Wilkinson, H. N., & Hardman, M. J. (2017). The role of estrogen in cutaneous ageing and repair. Maturitas, 103, 60-64. https://doi.org/10.1016/j.maturitas.2017.06.026

© 2017 Elsevier B.V. Combined advances in modern medical practice and increased human longevity are driving an ever-expanding elderly population. Females are particularly at risk of age-associated pathology, spending more of their lives in a post-men... Read More about The role of estrogen in cutaneous ageing and repair.

Cutaneous Nod2 expression regulates the skin microbiome and wound healing in a murine model (2017)
Journal Article
Williams, H., Crompton, R. A., Thomason, H. A., Campbell, L., Singh, G., McBain, A. J., …Hardman, M. J. (2017). Cutaneous Nod2 expression regulates the skin microbiome and wound healing in a murine model. Journal of Investigative Dermatology, 137(11), 2427-2436. https://doi.org/10.1016/j.jid.2017.05.029

The skin microbiome exists in dynamic equilibrium with the host but when the skin is compromised, bacteria can colonise the wound and impair wound healing. Thus the interplay between normal skin-microbial interactions versus pathogenic-microbial inte... Read More about Cutaneous Nod2 expression regulates the skin microbiome and wound healing in a murine model.

Comparing the effectiveness of polymer debriding devices using a porcine wound biofilm model (2016)
Journal Article
Wilkinson, H. N., McBain, A. J., Stephenson, C., & Hardman, M. J. (2016). Comparing the effectiveness of polymer debriding devices using a porcine wound biofilm model. Advances in wound care, 5(11), 475-485. https://doi.org/10.1089/wound.2015.0683

Objective: Debridement to remove necrotic and/or infected tissue and promote active healing remains a cornerstone of contemporary chronic wound management. While there has been a recent shift toward less invasive polymer-based debriding devices, thei... Read More about Comparing the effectiveness of polymer debriding devices using a porcine wound biofilm model.

Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair (2016)
Journal Article
Crompton, R., Williams, H., Ansell, D., Campbell, L., Holden, K., Cruickshank, S., & Hardman, M. J. (2016). Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair. Laboratory investigation, 96(4), 439-449. https://doi.org/10.1038/labinvest.2015.160

Wound infection is a major clinical problem, yet understanding of bacterial host interactions in the skin remains limited. Microbe-derived molecules, known as pathogen-associated molecular patterns, are recognised in barrier tissues by pattern-recogn... Read More about Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair.