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Methods to study the roles of Rho GTPases in platelet function (2018)
Journal Article
Rivero Crespo, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. Methods in molecular biology, 1821, 199-217. doi:10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

Methods to study the roles of Rho GTPases in platelet function (2018)
Book Chapter
Rivero, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. In Methods in Molecular Biology; Rho GTPases, 199-217. Humana Press. doi:10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

Reversal of stress fibre formation by Nitric Oxide mediated RhoA inhibition leads to reduction in the height of preformed thrombi (2018)
Journal Article
Atkinson, L., Yusuf, M. Z., Aburima, A., Ahmed, Y., Thomas, S. G., Naseem, K. M., & Calaminus, S. D. J. (2018). Reversal of stress fibre formation by Nitric Oxide mediated RhoA inhibition leads to reduction in the height of preformed thrombi. Scientific reports, 8(1), https://doi.org/10.1038/s41598-018-21167-6

© 2018 The Author(s). Evidence has emerged to suggest that thrombi are dynamic structures with distinct areas of differing platelet activation and inhibition. We hypothesised that Nitric oxide (NO), a platelet inhibitor, can modulate the actin cytosk... Read More about Reversal of stress fibre formation by Nitric Oxide mediated RhoA inhibition leads to reduction in the height of preformed thrombi.

Prostacyclin reverses platelet stress fibre formation causing platelet aggregate instability (2017)
Journal Article
Yusuf, M. Z., Raslan, Z., Atkinson, L., Aburima, A., Thomas, S. G., Naseem, K. M., & Calaminus, S. D. (2017). Prostacyclin reverses platelet stress fibre formation causing platelet aggregate instability. Scientific reports, 7(1), https://doi.org/10.1038/s41598-017-05817-9

Prostacyclin (PGI2) modulates platelet activation to regulate haemostasis. Evidence has emerged to suggest that thrombi are dynamic structures with distinct areas of differing platelet activation. It was hypothesised that PGI2 could reverse platelet... Read More about Prostacyclin reverses platelet stress fibre formation causing platelet aggregate instability.

CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells (2016)
Journal Article
Sinclair, A., Park, L., Shah, M., Drotar, M., Calaminus, S., Hopcroft, L. E., …Holyoake, T. L. (2016). CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood, 128(3), 371-383. doi:10.1182/blood-2015-08-661785

The regulation of hematopoietic stem cell (HSC) survival and self-renewal within the bone marrow (BM) niche is not well understood. We therefore investigated global transcriptomic profiling of normal human HSC/hematopoietic progenitor cells [HPCs], r... Read More about CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells.

Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane (2013)
Journal Article
Schachtner, H., Calaminus, S. D. J., Sinclair, A., Monypenny, J., Blundell, M. P., Leon, C., …Machesky, L. M. (2013). Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood, 121(13), 2542-2552. doi:10.1182/blood-2012-07-443457

Megakaryocytes give rise to platelets via extension of proplatelet arms, which are released through the vascular sinusoids into the bloodstream. Megakaryocytes and their precursors undergo varying interactions with the extracellular environment in th... Read More about Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane.

Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny (2012)
Journal Article
Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PloS one, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have emplo... Read More about Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

Actin on trafficking: could actin guide directed receptor transport? (2012)
Journal Article
Zech, T., Calaminus, S. D., & Machesky, L. M. (2012). Actin on trafficking: could actin guide directed receptor transport?. Cell adhesion & migration, 6(6), (476-481). doi:10.4161/cam.21373. ISSN 1933-6918

Here, we present emerging ideas surrounding the interplay between the actin cytoskeleton and receptor transport and activation. The bulk of actin dynamics in cells is thought to contribute to architecture and mobility. Actin also contributes to traff... Read More about Actin on trafficking: could actin guide directed receptor transport?.

Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo (2012)
Journal Article
Schachtner, H., Li, A., Stevenson, D., Calaminus, S. D., Thomas, S. G., Watson, S. P., …Machesky, L. M. (2012). Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European journal of cell biology, 91(11-12), (923-929). doi:10.1016/j.ejcb.2012.04.002. ISSN 0171-9335

We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblas... Read More about Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.

The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration (2011)
Journal Article
Zech, T., Calaminus, S., Caswell, P., Spence, H. J., Carnell, M., Insall, R. H., …Machesky, L. M. (2012). The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration. Journal of cell science, 124(22), 3753-3759. doi:10.1242/jcs.080986

The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and... Read More about The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration.

Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis (2011)
Journal Article
Carnell, M., Zech, T., Calaminus, S. D., Ura, S., Hagedorn, M., Johnston, S. A., …Insall, R. H. (2011). Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), (831-839). doi:10.1083/jcb.201009119. ISSN 0021-9525

WASP and SCAR homologue (WASH) is a recently identified and evolutionarily conserved regulator of actin polymerization. In this paper, we show that WASH coats mature Dictyostelium discoideum lysosomes and is essential for exocytosis of indigestible m... Read More about Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis.

Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading (2008)
Journal Article
Calaminus, S. D. J., Thomas, S., McCarty, O. J. T., Machesky, L. M., & Watson, S. P. (2008). Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading. Journal of thrombosis and haemostasis : JTH, 6(11), 1944-1952. doi:10.1111/j.1538-7836.2008.03141.x

During platelet spreading, the actin cytoskeleton undergoes marked changes, forming filopodia, lamellipodia and stress fibres. In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears p... Read More about Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading.


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