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Application of extracellular flux analysis for determining mitochondrial (2019)
Journal Article
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D. R., & Sturmey, R. G. (in press). Application of extracellular flux analysis for determining mitochondrial. Scientific reports,

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been rest... Read More

Optimising platelet secretomes to deliver robust tissue‐specific regeneration (2019)
Journal Article
Scully, D., Sfyri, P., Wilkinson, H. N., Acebes‐Huerta, A., Verpoorten, S., Muñoz‐Turrillas, M. C., …Matsakas, A. (in press). Optimising platelet secretomes to deliver robust tissue‐specific regeneration. Journal of tissue engineering and regenerative medicine, https://doi.org/10.1002/term.2965

Promoting cell proliferation is the cornerstone of most tissue regeneration therapies. As platelet‐based applications promote cell division and can be customised for tissue‐specific efficacy, this makes them strong candidates for developing novel r... Read More

Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis (2019)
Journal Article
Verpoorten, S., Sfyri, P., Scully, D., Mitchell, R., Tzimou, A., Mougios, V., …Matsakas, A. (in press). Loss of CD36 protects against diet‐induced obesity but results in impaired muscle stem cell function, delayed muscle regeneration and hepatic steatosis. Acta Physiologica, https://doi.org/10.1111/apha.13395

Aim: The prevalence of obesity is a major risk factor for cardiovascular and metabolic diseases including impaired skeletal muscle regeneration. Since skeletal muscle regenerative capacity is regulated by satellite cells, we aimed to investigate whet... Read More

Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22 (2019)
Journal Article
Wilkinson, H. N., Roberts, E. R., Stafford, A. R., Banyard, K. L., Matteucci, P., Mace, K. A., & Hardman, M. J. (2019). Tissue iron promotes wound repair via M2 macrophage polarisation and the chemokines CCL17 and CCL22. American Journal of Pathology, https://doi.org/10.1016/j.ajpath.2019.07.015

Macrophages are important for effective iron recycling and erythropoiesis, but they also play a crucial role in wound healing, orchestrating tissue repair. Recently, we demonstrated a significant accumulation of iron in healing wounds and a requireme... Read More

T-type Ca 2+ channel blocker mibefradil blocks ORAI channels via acting on extracellular surface (2019)
Journal Article
Li, P., Rubaiy, H. N., Chen, G., Hallett, T., Zaibi, N., Zeng, B., …Xu, S. (in press). T-type Ca 2+ channel blocker mibefradil blocks ORAI channels via acting on extracellular surface. British Journal of Pharmacology, https://doi.org/10.1111/bph.14788

Background and purpose Mibefradil (Mib), a T‐type Ca2+ channel blocker, has been investigated for treating solid tumours. However, its underlying mechanisms are still unclear. Here we aimed to investigate the pharmacological aspect of Mib on ORAI st... Read More

Germline selection shapes human mitochondrial DNA diversity (2019)
Journal Article
Wei, W., Tuna, S., Keogh, M. J., Smith, K. R., Aitman, T. J., Beales, P. L., …Chinnery, P. F. (2019). Germline selection shapes human mitochondrial DNA diversity. Science, 364(6442), https://doi.org/10.1126/science.aau6520

INTRODUCTION Only 2.4% of the 16.5-kb mitochondrial DNA (mtDNA) genome shows homoplasmic variation at >1% frequency in humans. Migration patterns have contributed to geographic differences in the frequency of common genetic variants, but population... Read More

Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality (2019)
Journal Article
Weldrick, P. J., Iveson, S., Hardman, M. J., & Paunov, V. N. (2019). Breathing new life into old antibiotics: Overcoming antibacterial resistance by antibiotic-loaded nanogel carriers with cationic surface functionality. Nanoscale, 11(21), 10472-10485. https://doi.org/10.1039/c8nr10022e

Multidrug-resistant pathogens are prevalent in chronic wounds. There is an urgent need to develop novel antimicrobials and formulation strategies that can overcome antibiotic resistance and provide a safe alternative to traditional antibiotics. This... Read More

Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood (2019)
Journal Article
Das, A. A., Medlock, J., Liang, H., Nees, D., Allsup, D. J., Madden, L. A., & Paunov, V. N. (2019). Bioimprint aided cell recognition and depletion of human leukemic HL60 cells from peripheral blood. Journal of materials chemistry B, Materials for biology and medicine /, 7(22), 3497-3504. https://doi.org/10.1039/c9tb00679f

We report a large scale preparation of bioimprints of layers of cultured human leukemic HL60 cells which can perform cell shape and size recognition from a mixture with peripheral blood mononuclear cells (PBMCs). We demonstrate that the bioimprint-ce... Read More

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOEC). An in vitro model using polyester mesh was established, allowing... Read More

Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling (2019)
Journal Article
Alyodawi, K., Vermeij, W., Omairi, S., Kretz, O., Solagna, F., Joch, B., …Hoeijmakers, J. (2019). Compression of morbidity in a progeroid mouse model through the attenuation of myostatin/activin signalling. Journal of Cachexia, Sarcopenia and Muscle, 10(3), 662-686. https://doi.org/10.1002/jcsm.12404

Background One of the principles underpinning our understanding of ageing is that DNA damage induces a stress response that shifts cellular resources from growth towards maintenance. A contrasting and seemingly irreconcilable view is that prompting... Read More

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART (2019)
Journal Article
Coticchio, G., Lagalla, C., Sturmey, R., Pennetta, F., & Borini, A. (2019). The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Human Reproduction Update, 25(4), 422–438. https://doi.org/10.1093/humupd/dmz008

Background: Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few... Read More

N-cinnamoylanthranilates as human TRPA1 modulators: Structure-activity relationships and channel binding sites (2019)
Journal Article
Chandrabalan, A., McPhillie, M. J., Morice, A. H., Boa, A. N., & Sadofsky, L. R. (2019). N-cinnamoylanthranilates as human TRPA1 modulators: Structure-activity relationships and channel binding sites. European Journal of Medicinal Chemistry, 170, 141-156. https://doi.org/10.1016/j.ejmech.2019.02.074

The transient receptor potential ankyrin 1 (TRPA1) channel is a non-selective cation channel, which detects noxious stimuli leading to pain, itch and cough. However, the mechanism(s) of channel modulation by many of the known, non-reactive modulators... Read More

Regulation of the dystrophin-associated glycoprotein complex composition by the metabolic properties of muscle fibres (2019)
Journal Article
Omairi, S., Hau, K., Collins-Hooper, H., Scott, C., Vaiyapuri, S., Torelli, S., …Patel, K. (2019). Regulation of the dystrophin-associated glycoprotein complex composition by the metabolic properties of muscle fibres. Scientific reports, 9(1), https://doi.org/10.1038/s41598-019-39532-4

The dystrophin-glycoprotein complex (DGC) links the muscle cytoskeleton to the extracellular matrix and is responsible for force transduction and protects the muscle fibres from contraction induced damage. Mutations in components of the DGC are respo... Read More

Survey of registered nurses’ selection of compression systems for the treatment of venous leg ulcers in the UK (2019)
Journal Article
Oates, A., & Adderley, U. (2019). Survey of registered nurses’ selection of compression systems for the treatment of venous leg ulcers in the UK. Journal of tissue viability, 28(2), 115-119. https://doi.org/10.1016/j.jtv.2019.02.004

Background: Venous leg ulceration is common in older adults in the United Kingdom. The gold-standard treatment is compression therapy. There are several compression bandage and hosiery systems that can be prescribed or purchased, but it was unclear w... Read More

Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3 (2019)
Journal Article
Berger, M., Riley, D. R. J., Lutz, J., Khalil, J. S., Aburima, A., Naseem, K. M., & Rivero, F. (2019). Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3. Cells, 8(2), 149. https://doi.org/10.3390/cells8020149

Typical Rho GTPases, like Rac1, Cdc42 and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB su... Read More

Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. (2019)
Journal Article
Wilkinson, H. N., Clowes, C., Banyard, K. L., Matteuci, P., Mace, K., & Hardman, M. J. (2019). Elevated local senescence in diabetic wound healing is linked to pathological repair via CXCR2. Journal of Investigative Dermatology, 139(5), 1171-1181.e6. https://doi.org/10.1016/j.jid.2019.01.005

Cellular senescence can be broadly defined as a stable, yet essentially irreversible, loss of proliferative capacity. Historically, senescence is described as a negative outcome of advanced cellular age. It is now clear, however, that senescence repr... Read More

Therapeutic targeting of the proinflammatory IL-6-JAK/STAT signalling pathways responsible for vascular restenosis in Type 2 diabetes mellitus (2019)
Journal Article
Florah, T., Moshapa, , Riches-Suman, K., Palmer, T. M., & Palmer, T. M. (2019). Therapeutic targeting of the proinflammatory IL-6-JAK/STAT signalling pathways responsible for vascular restenosis in Type 2 diabetes mellitus. Cardiology Research and Practice, 2019, doi:10.1155/2019/9846312

Type 2 diabetes mellitus (T2DM) is increasing worldwide, and it is associated with increased risk of coronary artery disease (CAD). For T2DM patients, the main surgical intervention for CAD is autologous saphenous vein grafting. However, T2DM patient... Read More

A769662 inhibits insulin-stimulated akt activation in human macrovascular endothelial cells independent of AMP-activated protein kinase (2018)
Journal Article
Strembitska, A., Mancini, S. J., Gamwell, J. M., Palmer, T. M., Baillie, G. S., & Salt, I. P. (2018). A769662 inhibits insulin-stimulated akt activation in human macrovascular endothelial cells independent of AMP-activated protein kinase. International Journal of Molecular Sciences, 19(12), 3886. doi:10.3390/ijms19123886

Protein kinase B (Akt) is a key enzyme in the insulin signalling cascade, required for insulin-stimulated NO production in endothelial cells (ECs). Previous studies have suggested that AMP-activated protein kinase (AMPK) activation stimulates NO synt... Read More

Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury (2018)
Journal Article
Scully, D., Sfyri, P., Verpoorten, S., Papadopoulos, P., Muñoz-Turrillas, M. C., Mitchell, R., …Matsakas, A. (2019). Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury. Acta Physiologica, 225(3), https://doi.org/10.1111/apha.13207

Aim The use of platelets as biomaterials has gained intense research interest. However, the mechanisms regarding platelet‐mediated skeletal myogenesis remain to be established. The aim of this study was to determine the role of platelet releasate in... Read More

Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity (2018)
Journal Article
Sfyri, P. P., Yuldasheva, N. Y., Tzimou, A., Giallourou, N., Crispi, V., Aburima, A., …Matsakas, A. (2018). Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity. Free radical biology & medicine, 129, 504-519. https://doi.org/10.1016...eeradbiomed.2018.10.422

Obesity leading to hyperlipidaemia and atherosclerosis is recognised to induce morphological and metabolic changes in many tissues. However, hyperlipidaemia can occur in the absence of obesity. The impact of the latter scenario on skeletal muscle and... Read More