All Outputs (935)

p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles (2013)
Journal Article
Ettelaie, C., ElKeeb, A. M., Maraveyas, A., & Collier, M. E. W. (2013). p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles. BBA - Molecular Cell Research, 1833(3), 613-621. https://doi.org/10.1016/j.bbamcr.2012.11.010

We previously showed that the phosphorylation of Ser253 within the cytoplasmic domain of human tissue factor (TF) initiates the incorporation and release of this protein into cell-derived microparticles. Furthermore, subsequent phosphorylation of Ser... Read More about p38 alpha phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles.

New targets for the immunotherapy of colon cancer - Does reactive disease hold the answer (2013)
Journal Article
Boncheva, V., Bonney, S. A., Brooks, S. E., Tangney, M., O'Sullivan, G., Mirnezami, A., & Guinn, B. A. (2013). New targets for the immunotherapy of colon cancer - Does reactive disease hold the answer. Cancer Gene Therapy, 20(3), 157-168. https://doi.org/10.1038/cgt.2013.5

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers in both men and women, posing a serious demographic and economic burden worldwide. In the United Kingdom, CRC affects 1 in every 20 people and it is often detected once well establ... Read More about New targets for the immunotherapy of colon cancer - Does reactive disease hold the answer.

[18F]-FLT Positron Emission Tomography Can Be Used to Image the Response of Sensitive Tumors to PI3-Kinase Inhibition with the Novel Agent GDC-0941 (2013)
Journal Article
Cawthorne, C., Burrows, N., Gieling, R. G., Morrow, C. J., Forster, D., Gregory, J., Radigois, M., Smigova, A., Babur, M., Simpson, K., Hodgkinson, C., Brown, G., McMahon, A., Dive, C., Hiscock, D., Wilson, I., & Williams, K. J. (2013). [18F]-FLT Positron Emission Tomography Can Be Used to Image the Response of Sensitive Tumors to PI3-Kinase Inhibition with the Novel Agent GDC-0941. Molecular Cancer Therapeutics, 12(5), 819-828. https://doi.org/10.1158/1535-7163.mct-12-0905

The phosphoinositide 3-kinase (PI3K) pathway is deregulated in a range of cancers, and several targeted inhibitors are entering the clinic. This study aimed to investigate whether the positron emission tomography tracer 3′-deoxy-3′-[ 18 F]fluorothymi... Read More about [18F]-FLT Positron Emission Tomography Can Be Used to Image the Response of Sensitive Tumors to PI3-Kinase Inhibition with the Novel Agent GDC-0941.

Glycoprotein Ibα and FcγRIIa play key roles in platelet activation by the colonizing bacterium, Streptococcus oralis (2013)
Journal Article
Tilley, D. O., Arman, M., Smolenski, A., Cox, D., O'Donnell, J. S., Douglas, C. W., Watson, S. P., & Kerrigan, S. W. (2013). Glycoprotein Ibα and FcγRIIa play key roles in platelet activation by the colonizing bacterium, Streptococcus oralis. Journal of thrombosis and haemostasis : JTH, 11(5), 941-950. https://doi.org/10.1111/jth.12175

Background: Infective endocarditis (IE) is characterized by thrombus formation on a cardiac valve. The oral bacterium, Streptococcus oralis, is recognized for its ability to colonize damaged heart valves and is frequently isolated from patients with... Read More about Glycoprotein Ibα and FcγRIIa play key roles in platelet activation by the colonizing bacterium, Streptococcus oralis.

A Method for Positive and Negative Selection of Plasmodium falciparum Platelet-Mediated Clumping Parasites and Investigation of the Role of CD36 (2013)
Journal Article
Arman, M., Adams, Y., Lindergard, G., & Rowe, J. A. (2013). A Method for Positive and Negative Selection of Plasmodium falciparum Platelet-Mediated Clumping Parasites and Investigation of the Role of CD36. PLoS ONE, 8(2), Article e55453. https://doi.org/10.1371/journal.pone.0055453

Platelet-mediated clumping of Plasmodium falciparum infected erythrocytes (IEs) is a frequently observed parasite adhesion phenotype. The importance of clumping in severe malaria and the molecular mechanisms behind this phenomenon are incompletely un... Read More about A Method for Positive and Negative Selection of Plasmodium falciparum Platelet-Mediated Clumping Parasites and Investigation of the Role of CD36.

Innovative evolution of cancer gene and cellular therapies (2013)
Journal Article
Lam, P., Khan, G., Stripecke, R., Hui, K. M., Kasahara, N., Peng, K. W., & Guinn, B. A. (2013). Innovative evolution of cancer gene and cellular therapies. Cancer Gene Therapy, 20(3), 141-149. https://doi.org/10.1038/cgt.2012.93

We provide an overview of the latest developments in cancer gene therapy - from the bench to early-stage clinical trials. We describe the most recent work of worldwide teams including experienced scientists and clinicians, reflecting the recent emerg... Read More about Innovative evolution of cancer gene and cellular therapies.

GxcC connects Rap and Rac signaling during Dictyostelium development (2013)
Journal Article
Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P. J., & Kortholt, A. (2013). GxcC connects Rap and Rac signaling during Dictyostelium development. BMC cell biology, 14(1), Article 6. https://doi.org/10.1186/1471-2121-14-6

Background: Rap proteins belong to the Ras family of small G-proteins. Dictyostelium RapA is essential and implicated in processes throughout the life cycle. In early development and chemotaxis competent cells RapA induces pseudopod formation by acti... Read More about GxcC connects Rap and Rac signaling during Dictyostelium development.

Adrenomedullin haploinsufficiency predisposes to secondary lymphedema (2013)
Journal Article
Nikitenko, L. L., Shimosawa, T., Henderson, S., Mäkinen, T., Shimosawa, H., Qureshi, U., Pedley, R. B., Rees, M. C., Fujita, T., & Boshoff, C. (2013). Adrenomedullin haploinsufficiency predisposes to secondary lymphedema. Journal of Investigative Dermatology, 133(7), 1768-1776. https://doi.org/10.1038/jid.2013.47

Secondary lymphedema is a debilitating condition, and genetic factors predisposing to its development remain largely unknown. Adrenomedullin (AM) is peptide encoded, together with proadrenomedullin N-terminal peptide (PAMP), by the Adm gene (adrenome... Read More about Adrenomedullin haploinsufficiency predisposes to secondary lymphedema.

Proteins and lipids of glycosomal membranes from Leishmania tarentolae and Trypanosoma brucei [version 1; peer review: 2 approved] (2013)
Journal Article
Colasante, C., Voncken, F., Manful, T., Ruppert, T., Tielens, A. G. M., van Hellemond, J. J., & Clayton, C. (2013). Proteins and lipids of glycosomal membranes from Leishmania tarentolae and Trypanosoma brucei [version 1; peer review: 2 approved]. F1000Research, 2, Article 27. https://doi.org/10.3410/f1000research.2-27.v1

In kinetoplastid protists, several metabolic pathways, including glycolysis and purine salvage, are located in glycosomes, which are microbodies that are evolutionarily related to peroxisomes. With the exception of some potential transporters for fat... Read More about Proteins and lipids of glycosomal membranes from Leishmania tarentolae and Trypanosoma brucei [version 1; peer review: 2 approved].

Corrigendum (2013)
Journal Article
Waxman, J., Cobb, J. P., Palmieri, C., Rhim, J. S., Pchejetski, D., Mauri, F., Sandison, A., Fonseca, A. V., Rodriguez-Teja, M., Adamarek, A., Gronau, J. H., Kogianni, G., Caley, M. P., & Sturge, J. (2013). Corrigendum. Journal of Pathology, 229(3), e4-e4. https://doi.org/10.1002/path.4156

Proteomic (antibody microarray) exploration of the molecular mechanism of action of the specific COX-2 inhibitor DuP 697 (2013)
Journal Article
Agarwal, V., Hodgkinson, V. C., Eagle, G. L., Scaife, L., Lind, M. J., & Cawkwell, L. (2013). Proteomic (antibody microarray) exploration of the molecular mechanism of action of the specific COX-2 inhibitor DuP 697. International Journal of Oncology, 42(3), 1088-1092. https://doi.org/10.3892/ijo.2013.1784

We have previously shown that specific COX-2 inhibitors, including DuP 697, have anti-proliferative effects on mesothelioma cells and potentiate the cytotoxicity of pemetrexed. Here, we used a novel proteomic approach to explore the mechanism of acti... Read More about Proteomic (antibody microarray) exploration of the molecular mechanism of action of the specific COX-2 inhibitor DuP 697.

Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction (2013)
Journal Article
Omelia, E. J., Uchimoto, M. L., & Williams, G. (2013). Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction. Analytical Biochemistry, 435(2), 120-122. https://doi.org/10.1016/j.ab.2012.12.024

The use of mRNA for the identification of body fluids is of particular interest in forensic science, and increasing support has been demonstrated for the use of microRNA (miRNA) analysis. MiRNA is more stable than mRNA and has been shown to be differ... Read More about Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction.

A Novel Missense Mutation, I890T, in the Pore Region of Cardiac Sodium Channel Causes Brugada Syndrome (2013)
Journal Article
Tarradas, A., Selga, E., Beltran-Alvarez, P., Pérez-Serra, A., Riuró, H., Picó, F., Iglesias, A., Campuzano, O., Castro-Urda, V., Fernández-Lozano, I., Pérez, G. J., Scornik, F. S., & Brugada, R. (2013). A Novel Missense Mutation, I890T, in the Pore Region of Cardiac Sodium Channel Causes Brugada Syndrome. PLoS ONE, 8(1), Article e53220. https://doi.org/10.1371/journal.pone.0053220

Brugada syndrome (BrS) is a life-threatening, inherited arrhythmogenic syndrome associated with autosomal dominant mutations in SCN5A, the gene encoding the cardiac Na+ channel alpha subunit (Nav1.5). The aim of this work was to characterize the func... Read More about A Novel Missense Mutation, I890T, in the Pore Region of Cardiac Sodium Channel Causes Brugada Syndrome.

TRPC Channels and Their Splice Variants are Essential for Promoting Human Ovarian Cancer Cell Proliferation and Tumorigenesis (2013)
Journal Article
Zeng, B., Yuan, C., Yang, X., Atkin, S. L., & Xu, S.-Z. (2013). TRPC Channels and Their Splice Variants are Essential for Promoting Human Ovarian Cancer Cell Proliferation and Tumorigenesis. Current Cancer Drug Targets, 13(1), 103-116. https://doi.org/10.2174/156800913804486629

TRPC channels are Ca2+-permeable cationic channels controlling Ca2+ influx response to the activation of G protein-coupled receptors and protein tyrosine kinase pathways or the depletion of Ca2+ stores. Here we aimed to investigate whether TRPC can a... Read More about TRPC Channels and Their Splice Variants are Essential for Promoting Human Ovarian Cancer Cell Proliferation and Tumorigenesis.

Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions (2013)
Book Chapter
Scrace, S., O'Neill, E., Hammond, E. M., & Pires, I. M. (2013). Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions. In A. S. Coutts (Ed.), Adhesion Protein Protocols (295-306). Humana Press. https://doi.org/10.1007/978-1-62703-538-5_17

Investigation of the mechanisms behind the regulation of cellular motility and adhesion is key to understanding metastasis and the biology of tumor spreading. There are many technologies available for these studies, but the majority of them are eithe... Read More about Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions.

MRC2 (2012)
Book Chapter
Gronau, J. H., Isacke, C. M., & Sturge, J. (2012). MRC2. In S. Choi (Ed.), Encyclopedia of Signaling Molecules (1119-1123). Springer. https://doi.org/10.1007/978-1-4419-0461-4_79

Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny (2012)
Journal Article
Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R., & Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have emplo... Read More about Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

Expression and localization of creatine kinase in the preimplantation embryo (2012)
Journal Article
Forsey, K. E., Ellis, P. J., Sargent, C. A., Sturmey, R. G., & Leese, H. J. (2013). Expression and localization of creatine kinase in the preimplantation embryo. Molecular Reproduction and Development, 80(3), 185-192. https://doi.org/10.1002/mrd.22146

Creatine Kinase (CK) catalyses the “creatine shuttle,” the reversible conversion of creatine phosphate to creatine with the liberation of ATP. This article examines the potential role of the creatine shuttle in the provision of ATP during mouse preim... Read More about Expression and localization of creatine kinase in the preimplantation embryo.

Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer (2012)
Journal Article
Palmieri, C., Caley, M. P., Purshouse, K., Fonseca, A.-V., Rodriguez-Teja, M., Kogianni, G., Woodley, L., Odendaal, J., Elliott, K., Waxman, J., & Sturge, J. (2013). Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer. The British Journal of Cancer, 108(1), 163-169. https://doi.org/10.1038/bjc.2012.540

Background:Endo180 (CD280; MRC2; uPARAP)-dependent collagen remodelling is dysregulated in primary tumours and bone metastasis. Here, we confirm the release and diagnostic accuracy of soluble Endo180 for diagnosing metastasis in breast cancer (BCa).M... Read More about Endo180 modulation by bisphosphonates and diagnostic accuracy in metastatic breast cancer.

PTEN protein expression in malignant pleural mesothelioma (2012)
Journal Article
Agarwal, V., Campbell, A., Beaumont, K. L., Cawkwell, L., & Lind, M. J. (2013). PTEN protein expression in malignant pleural mesothelioma. Tumor Biology, 34(2), 847-851. https://doi.org/10.1007/s13277-012-0615-9

Malignant pleural mesothelioma is associated with poor prognosis and despite recent advances in chemotherapy, the median survival is still approximately 12 months. Loss of phosphatase and tensin homolog (PTEN) protein expression may lead to constitut... Read More about PTEN protein expression in malignant pleural mesothelioma.