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All Outputs (18)

Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices (2021)
Journal Article
Christensen, M. G., Cawthorne, C., Dyer, C. E., Greenman, J., & Pamme, N. (2021). Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices. Microfluidics and Nanofluidics, 25(4), Article 35. https://doi.org/10.1007/s10404-021-02434-x

Microfluidic ‘organ-on-a-chip’ devices hold great potential for better mimicking the continuous flow microenvironment experienced by tissue and cells in vivo, thereby ensuring realistic transport of nutrients and elimination of waste products. Howeve... Read More about Investigating oxygen transport efficiencies in precision-cut liver slice-based organ-on-a-chip devices.

The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart (2019)
Journal Article
Onwuli, D. O., Samuel, S., Sfyri, P., Welham, K., Goddard, M., Abu-Omar, Y., …Beltran-Alvarez, P. (2019). The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. International journal of cardiology, 282, 76-80. https://doi.org/10.1016/j.ijcard.2019.01.102

Background The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-tra... Read More about The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart.

Amino acid based gallium-68 chelators capable of radiolabeling at neutral pH (2017)
Journal Article
Kubicek, V., Bohmova, Z., Price, T. W., Gallo, J., Kubíček, V., Böhmová, Z., …Stasiuk, G. J. (2017). Amino acid based gallium-68 chelators capable of radiolabeling at neutral pH. Dalton Transactions : an international journal of inorganic chemistry, 46(48), 16973-16982. https://doi.org/10.1039/c7dt03398b

Gallium-68 ( 68 Ga) has been the subject of increasing interest for its potential in the production of radiotracers for diagnosis of diseases. In this work we report the complexation of 68 Ga by the amino acid based tripodal chelate H 3 Dpaa, and two... Read More about Amino acid based gallium-68 chelators capable of radiolabeling at neutral pH.

Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity (2017)
Journal Article
Spagnul, C., Turner, L. C., Giuntini, F., Greenman, J., & Boyle, R. W. (2017). Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity. Journal of Materials Chemistry B, 5(9), 1834-1845. https://doi.org/10.1039/c6tb03198f

Spectroscopic and photodynamic properties of three novel polymeric hydrogels bearing porphyrins have been studied in vitro on the recombinant bioluminescent Gram-negative Escherichia coli DH5α to assess their ability to inactivate bacterial strains i... Read More about Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity.

On-chip determination of C-reactive protein using magnetic particles in continuous flow (2014)
Journal Article
Phurimsak, C., Tarn, M. D., Peyman, S. A., Greenman, J., & Pamme, N. (2014). On-chip determination of C-reactive protein using magnetic particles in continuous flow. Analytical chemistry, 86(21), 10552-10559. https://doi.org/10.1021/ac5023265

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the... Read More about On-chip determination of C-reactive protein using magnetic particles in continuous flow.

Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions (2013)
Journal Article
Pacelli, A., Greenman, J., Cawthorne, C., & Smith, G. (2014). Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions. Journal of Labelled Compounds and Radiopharmaceuticals, 57(4), 317-322. https://doi.org/10.1002/jlcr.3160

The role of cyclooxygenase (COX)-2 as a driving force in early tumourigenesis and the current interest in the combination of COX-2 inhibitors with standard therapy in clinical trials creates an urgent need to establish clinically relevant diagnostic... Read More about Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions.

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device (2012)
Journal Article
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., …Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chi... Read More about Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device.

Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’” (2011)
Journal Article
Zheng, S., Webster, A., Zheng, S., Welham, K. J., Dyer, C. E., Greenman, J., …Haswell, S. J. (2011). Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”. Analyst, 136(2), 411-411. https://doi.org/10.1039/c0an00729c

Comment by Tsikas et al. (Analyst, 2011, DOI: 10.1039/c0an00411a) on the preliminary work by Shen and colleagues (Analyst, 2010, 135, 302) describing the use of ESI-MS/MS for the detection of methylpiperazinobenzenediamine, as a probe for NO in tissu... Read More about Reply to “Analysis of NO and its metabolites by mass spectrometry. Comment on ‘Detection of nitric oxide in tissue samples by ESI-MS’”.

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), 3188-3195. https://doi.org/10.1002/elps.201100172

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More about On-chip integrated labelling, transport and detection of tumour cells.

Microsystems for personalized biomolecular diagnostics (2011)
Journal Article
Shaw, K. J., Birch, C., Hughes, E. M., Jakes, A. D., Greenman, J., & Haswell, S. J. (2011). Microsystems for personalized biomolecular diagnostics. Engineering in Life Sciences, 11(2), 121-132. https://doi.org/10.1002/elsc.201000175

The development of microfluidic methodology that can be used in conjunction with drug screening and biomolecular diagnostics offers a route to evidence-based personalized medical care. Ideally, all personal diagnostics are best carried out in a rapid... Read More about Microsystems for personalized biomolecular diagnostics.

Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device (2010)
Journal Article
Shaw, K. J., Joyce, D., Docker, P. T., Dyer, C. E., Greenway, G. M., Greenman, J., & Haswell, S. J. (2011). Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device. Lab on a chip, 11(3), 443-448. https://doi.org/10.1039/c0lc00346h

Integrated DNA extraction and amplification have been carried out in a microfluidic device using electro-osmotic pumping (EOP) for fluidic control. All the necessary reagents for performing both DNA extraction and polymerase chain reaction (PCR) ampl... Read More about Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device.

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More about Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling.

Detection of nitric oxide in tissue samples by ESI-MS (2009)
Journal Article
Shen, Z., Webster, A., Welham, K. J., Dyer, C. E., Greenman, J., & Haswell, S. J. (2010). Detection of nitric oxide in tissue samples by ESI-MS. Analyst, 135(2), 302-305. https://doi.org/10.1039/b917643h

A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chose... Read More about Detection of nitric oxide in tissue samples by ESI-MS.

CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds (2009)
Journal Article
Khan, A., Nicholson, G., McRobbie, G., Greenman, J., Pannecouque, C., Daelemans, D., …Archibald, S. J. (2009). CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds. Antiviral research, 82(2), A59-A60. https://doi.org/10.1016/j.antiviral.2009.02.141

AMD3100 is a bis-azamacrocyclic compound that has been demonstrated to be a highly effective antagonist of the CXCR4 chemokine receptor. The two azamacrocyclic rings have been shown to interact with aspartate residues on the receptor via hydrogen bon... Read More about CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds.

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More about The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.

Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device (2009)
Journal Article
Oakley, J. A., Shaw, K. J., Docker, P. T., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device. Lab on a chip, 9(11), 1596-1600. https://doi.org/10.1039/b820553a

A silica monolith used to support both electro-osmotic pumping (EOP) and the extraction/elution of DNA coupled with gel-supported reagents is described. The benefits of the combined EOP extraction/elution system were illustrated by combining DNA extr... Read More about Development of a bi-functional silica monolith for electro-osmotic pumping and DNA clean-up/extraction using gel-supported reagents in a microfluidic device.

Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes (2009)
Journal Article
Khan, A., Nicholson, G., McRobbie, G., Pannecouque, C., De Clercq, E., Ullom, R., …Greenman, J. (2009). Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes. Journal of the American Chemical Society, 131(10), 3416-3417. https://doi.org/10.1021/ja807921k

A new copper(II) containing bis-macrocyclic CXCR4 chemokine receptor antagonist is shown to have improved binding properties to the receptor protein in comparison to the drug AMD3100 (Plerixafor, Mozobil). The interaction of the metallodrug has been... Read More about Binding Optimization through Coordination Chemistry: CXCR4 Chemokine Receptor Antagonists from Ultrarigid Metal Complexes.

Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device (2009)
Journal Article
Shaw, K. J., Docker, P. T., Haswell, S. J., Joyce, D. A., Dyer, C. E., Greenman, J., & Greenway, G. M. (2009). Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device. Lab on a chip, 9(23), 3430-3432. https://doi.org/10.1039/b913309g

A novel DNA loading methodology is presented for performing DNA extraction on a microfluidic system. DNA in a chaotropic salt solution was manually loaded onto a silica monolith orthogonal to the subsequent flow of wash and elution solutions. DNA was... Read More about Simple practical approach for sample loading prior to DNA extraction using a silica monolith in a microfluidic device.