University of Hull logo

A novel microfluidic device capable of maintaining functional thyroid carcinoma (2019)
Journal Article
Riley, A., Green, V., Cheah, R., McKenzie, G., Karsai, L., England, J., & Greenman, J. (2019). A novel microfluidic device capable of maintaining functional thyroid carcinoma. BMC Cancer, 19, https://doi.org/10.1186/s12885-019-5465-z

Background Though the management of malignancies has improved vastly in recent years, many treatment options lack the desired efficacy and fail to adequately augment patient morbidity and mortality. It is increasingly clear that patient response to... Read More

The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart (2019)
Journal Article
Onwuli, D. O., Samuel, S., Sfyri, P., Welham, K., Goddard, M., Abu-Omar, Y., …Beltran-Alvarez, P. (2019). The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. International journal of cardiology, 282, 76-80. https://doi.org/10.1016/j.ijcard.2019.01.102

Background The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-tra... Read More

Innovative organotypic in vitro models for safety assessment: aligning with regulatory requirements and understanding models of the heart, skin, and liver as paradigms (2018)
Journal Article
Pridgeon, C. S., Schlott, C., Wong, M. W., Heringa, M. B., Heckel, T., Leedale, J., …Goldring, C. E. P. (2018). Innovative organotypic in vitro models for safety assessment: aligning with regulatory requirements and understanding models of the heart, skin, and liver as paradigms. Archives of Toxicology, 92(2), 557-569. https://doi.org/10.1007/s00204-018-2152-9

The development of improved, innovative models for the detection of toxicity of drugs, chemicals, or chemicals in cosmetics is crucial to efficiently bring new products safely to market in a cost-effective and timely manner. In addition, improvement... Read More

Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles (2017)
Journal Article
Ettelaie, C., Collier, M., Featherby, S., Greenman, J., & Maraveyas, A. (2017). Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles. BBA - Molecular Cell Research, 1865(1), 12-24. doi:10.1016/j.bbamcr.2017.09.016

© 2017 Elsevier B.V. The exposure and release of TF is regulated by post-translational modifications of its cytoplasmic domain. Here, the potential of Pin1 to interact with the cytoplasmic domain of TF, and the outcome on TF function was examined. MD... Read More

Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Michno, A., Greenman, J., & Stafford, N. D. (2012). Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results in immunology, 2, (1-6). doi:10.1016/j.rinim.2011.12.001. ISSN 2211-2839

The aim of this study was to determine the effect of HNSCC tumour treatment on systemic Th1 and Th2 cytokine levels and investigate correlations with clinicopathological parameters. IL2, IL4, IL5, IL6, IL8, IL10, IL13, GMCSF, IFNγ and TNF&alpha... Read More

A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs (2011)
Journal Article
Hattersley, S. M., Sylvester, D. C., Dyer, C. E., Stafford, N. D., Haswell, S. J., & Greenman, J. (2012). A microfluidic system for testing the responses of head and neck squamous cell carcinoma tissue biopsies to treatment with chemotherapy drugs. Annals of biomedical engineering, 40(6), 1277-1288. doi:10.1007/s10439-011-0428-9

Tumors are heterogeneous masses of cells characterized pathologically by their size and spread. Their chaotic biology makes treatment of malignancies hard to generalize. We present a robust and reproducible glass microfluidic system, for the maintena... Read More

Microsystems for personalized biomolecular diagnostics (2011)
Journal Article
Shaw, K. J., Birch, C., Hughes, E. M., Jakes, A. D., Greenman, J., & Haswell, S. J. (2011). Microsystems for personalized biomolecular diagnostics. Engineering in Life Sciences, 11(2), 121-132. doi:10.1002/elsc.201000175

The development of microfluidic methodology that can be used in conjunction with drug screening and biomolecular diagnostics offers a route to evidence-based personalized medical care. Ideally, all personal diagnostics are best carried out in a rapid... Read More

Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device (2010)
Journal Article
Shaw, K. J., Joyce, D., Docker, P. T., Dyer, C. E., Greenway, G. M., Greenman, J., & Haswell, S. J. (2011). Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device. Lab on a chip, 11(3), 443-448. https://doi.org/10.1039/c0lc00346h

Integrated DNA extraction and amplification have been carried out in a microfluidic device using electro-osmotic pumping (EOP) for fluidic control. All the necessary reagents for performing both DNA extraction and polymerase chain reaction (PCR) ampl... Read More

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action (2010)
Journal Article
Smith, K., Malatesti, N., Cauchon, N., Hunting, D., Lecomte, R., van Lier, J. E., …Boyle, R. W. (2011). Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology, 132(2), 256-265. https://doi.org/10.1111/j.1365-2567.2010.03359.x

Two cationic porphyrins bearing an isothiocyanate group for conjugation to monocolonal antibodies have been synthesized. The two porphyrins conjugated efficiently to three monoclonal antibodies (anti-CD104, anti-CD146 and anti-CD326), which recognize... Read More

Development of microfluidic devices for biomedical and clinical application (2010)
Journal Article
Webster, A., Greenman, J., & Haswell, S. J. (2011). Development of microfluidic devices for biomedical and clinical application. Journal of Chemical Technology and Biotechnology, 86(1), 10-17. doi:10.1002/jctb.2482

This review focuses on the development and use of microfluidic devices within a clinical setting. The underlying theoretical background of microfluidics is briefly elucidated. The materials and techniques used to fabricate the devices and their appli... Read More

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More

Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing (2009)
Journal Article
Dou, Y., Haswell, S., Greenman, J., & Wadhawan, J. (2009). Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing. Electrochemistry communications, 11(10), (1976-1981). doi:10.1016/j.elecom.2009.08.032. ISSN 1388-2481

The detection of hydrogen peroxide is detailed using horseradish peroxidase and anthraquinone. Both species are immobilized on a glassy carbon electrode substrate. This dual immobilization gives rise to lower detection limits compared with the situat... Read More

Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser (2009)
Journal Article
Oakley, J. A., Robinson, S., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser. Analytica Chimica Acta, 652(1-2), 239-244. https://doi.org/10.1016/j.aca.2009.07.063

An integrated gel supported micro-fluidic system is reported, in which PCR products can be efficiently injected into a capillary electrophoresis device. The gel supported system is designed to provide greater stability to reagents during long periods... Read More

COX-2 specific inhibitors enhance the cytotoxic effects of pemetrexed in mesothelioma cell lines (2009)
Journal Article
O'Kane, S. L., Eagle, G. L., Greenman, J., Lind, M. J., & Cawkwell, L. (2010). COX-2 specific inhibitors enhance the cytotoxic effects of pemetrexed in mesothelioma cell lines. Lung Cancer, 67(2), (160-165). doi:10.1016/j.lungcan.2009.04.008. ISSN 0169-5002

BACKGROUND Although malignant pleural mesothelioma is a rare tumour, its incidence is increasing. The prognosis remains very poor with an average survival of 10 months from diagnosis. The choice of chemotherapy regimens for mesothelioma patients is l... Read More