University of Hull logo

Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals (2019)
Journal Article
Ghazali, M., Harper, L. R., Lawson Handley, L., Carpenter, A. I., Murray-Dickson, G., Di Muri, C., …Hänfling, B. (2019). Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals. Biological Conservation, 238(October 2019), https://doi.org/10.1016/j.biocon.2019.108225

© 2019 Elsevier Ltd Environmental DNA (eDNA) metabarcoding can identify terrestrial taxa utilising aquatic habitats alongside aquatic communities, but terrestrial species' eDNA dynamics are understudied. We evaluated eDNA metabarcoding for monitoring... Read More

Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types (2018)
Journal Article
Sellers, G. S., Di Muri, C., Gómez, A., & Hänfling, B. (2018). Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types. Metabarcoding and Metagenomics, 2, e24556. https://doi.org/10.3897/mbmg.2.24556

Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of hi... Read More

The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding (2018)
Journal Article
Li, J., Lawson Handley, L. J., Read, D. S., & Hänfling, B. (2018). The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding. Molecular Ecology Resources, 18(5), 1102-1114. https://doi.org/10.1111/1755-0998.12899

© 2018 John Wiley & Sons Ltd. Environmental DNA (eDNA) is a promising tool for rapid and noninvasive biodiversity monitoring. eDNA density is low in environmental samples, and a capture method, such as filtration, is often required to concentrate e... Read More

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Gough, K. C., …Hänfling, B. (2018). Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus). Ecology and Evolution, 8(12), 6330-6341. https://doi.org/10.1002/ece3.4013

Environmental DNA (eDNA) analysis is a rapid, cost‐effective, non‐invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species‐specific survey tool for rar... Read More

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive (2018)
Journal Article
Hering, D., Borja, A., Jones, J. I., Pont, D., Boets, P., Bouchez, A., …Kelly, M. (2018). Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive. Water Research, 138, 192-205. https://doi.org/10.1016/j.watres.2018.03.003

Assessment of ecological status for the European Water Framework Directive (WFD) is based on “Biological Quality Elements” (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is... Read More

Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK (2017)
Journal Article
Blackman, R. C., Constable, D., Hahn, C., Sheard, A. M., Durkota, J., Hänfling, B., & Handley, L. L. (2017). Detection of a new non-native freshwater species by DNA metabarcoding of environmental samples — first record of Gammarus fossarum in the UK. Aquatic Invasions, 12(2), 177-189. https://doi.org/10.3391/ai.2017.12.2.06

© 2017 The Author(s). and 2017 REABIC. We report the discovery of a non-native gammarid, Gammarus fossarum (Koch, 1836) (Crustacea, Amphipoda), in UK rivers. Gammarus fossarum is a common freshwater gammarid in many parts of mainland Europe, but was... Read More

Genetic evidence challenges the native status of a threatened freshwater fish (Carassius carassius) in England (2017)
Journal Article
Jeffries, D. L., Copp, G. H., Maes, G. E., Lawson Handley, L., Sayer, C. D., & Hänfling, B. (2017). Genetic evidence challenges the native status of a threatened freshwater fish (Carassius carassius) in England. Ecology and Evolution, 7(9), 2871-2882. https://doi.org/10.1002/ece3.2831

© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. A fundamental consideration for the conservation of a species is the extent of its native range, that is, regions naturally colonized. However, both natural processes and... Read More

The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545 (2015)
Journal Article
Hutchinson, W. F., Culling, M., Orton, D. C., Hänfling, B., Handley, L. L., Hamilton-Dyer, S., …Barrett, J. H. (2015). The globalization of naval provisioning: ancient DNA and stable isotope analyses of stored cod from the wreck of the Mary Rose, AD 1545. Royal Society Open Science, 2(9), 150199. https://doi.org/10.1098/rsos.150199

© 2015 The Authors. A comparison of ancient DNA (single-nucleotide polymorphisms) and carbon and nitrogen stable isotope evidence suggests that stored cod provisions recovered from the wreck of the Tudor warship Mary Rose, which sank in the Solent, s... Read More

Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis (2015)
Journal Article
Mathers, T. C., Hammond, R. L., Jenner, R., Hänfling, B., Atkins, J., & Gómez, A. (2015). Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity, 115(1), 37-46. https://doi.org/10.1038/hdy.2015.10

© 2015 Macmillan Publishers Limited All rights reserved. Transitions in sexual system and reproductive mode may affect the course of sex chromosome evolution, for instance by altering the strength of sexually antagonistic selection. However, there ha... Read More

Multiple global radiations in tadpole shrimps challenge the concept of 'living fossils' (2013)
Journal Article
Mathers, T. C., Hammond, R. L., Jenner, R. A., Hänfling, B., & Gomez, A. (2013). Multiple global radiations in tadpole shrimps challenge the concept of 'living fossils'. PeerJ, 1, e62. https://doi.org/10.7717/peerj.62

‘Living fossils’, a phrase first coined by Darwin, are defined as species with limited recent diversification and high morphological stasis over long periods of evolutionary time. Morphological stasis, however, can potentially lead to div... Read More

Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range (2009)
Journal Article
Sui, L., Zhang, F., Wang, X., Bossier, P., Sorgeloos, P., & Hänfling, B. (2009). Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range. Marine Biology, 156(8), 1573-1583. https://doi.org/10.1007/s00227-009-1193-2

The Chinese mitten crab Eriocheir sinensis is an indigenous and economically important species in China, but can also be found as invasive species in Europe and America. Mitten crabs have been exploited extensively as a food resource since the 1990s.... Read More

Understanding the establishment success of non-indigenous fishes: lessons from population genetics (2007)
Journal Article
Hänfling, B. (2007). Understanding the establishment success of non-indigenous fishes: lessons from population genetics. Journal of fish biology, 71(SUPPL. D), 115-135. doi:10.1111/j.1095-8649.2007.01685.x

During the last 10 years, an increasing number of studies have explored evolutionary aspects ofbiological invasions. It is becoming increasingly clear that evolutionary processes play animportant role during the establishment of non-native species. G... Read More

Genetic population structure and contemporary dispersal patterns of a recent European invader, the Chinese mitten crab, Eriocheir sinensis (2006)
Journal Article
Herborg, L. M., Weetman, D., Van Oosterhout, C., & Hänfling, B. (2007). Genetic population structure and contemporary dispersal patterns of a recent European invader, the Chinese mitten crab, Eriocheir sinensis. Molecular ecology, 16(2), 231-242. https://doi.org/10.1111/j.1365-294x.2006.03133.x

Genetic studies of recently established populations are challenging because the assumption of equilibrium underlying many analyses is likely to be violated. Using microsatellites, we investigated determinants of genetic structure and migration among... Read More

Concordant genetic estimators of migration reveal anthropogenically enhanced source-sink population structure in the River Sculpin, Cottus gobio (2006)
Journal Article
Hänfling, B., & Weetman, D. (2006). Concordant genetic estimators of migration reveal anthropogenically enhanced source-sink population structure in the River Sculpin, Cottus gobio. Genetics, 173(3), 1487-1501. doi:10.1534/genetics.105.054296

River systems are vulnerable to natural and anthropogenic habitat fragmentation and will often harbor populations deviating markedly from simplified theoretical models. We investigated fine-scale population structure in the sedentary river fish Cottu... Read More

Impact of habitat fragmentation on genetic population structure of roach, Rutilus rutilus, in a riparian ecosystem (2004)
Journal Article
Hänfling, B., Durka, W., & Brandl, R. (2004). Impact of habitat fragmentation on genetic population structure of roach, Rutilus rutilus, in a riparian ecosystem. Conservation genetics, 5(2), 247-257. https://doi.org/10.1023...OGE.0000030008.20492.2c

During the last 200 years, the riparian ecosystem along major rivers has been reduced to a few scattered remnants. Important elements of the riparian ecosystem are water bodies which were originally connected to the main river channel by annual flood... Read More