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Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya (2019)
Journal Article
Ngamsom, B., Wandera, E. . . A., Iles, A., Kimani, R., Muregi, F., Gitaka, J., & Pamme, N. (2019). Rapid Detection of Group B Streptococcus (GBS) from artificial urine samples based on IFAST and ATP Bioluminescence Assay: from development to practical challenges during protocol testing in Kenya. Analyst, https://doi.org/10.1039/c9an01808e

We report the rapid detection (20 min) of Streptococcus agalactiae, Group B Streptococcus (GBS) employing on-chip magnetic isolation of GBS based on immiscible filtration assisted by surface tension (IFAST), followed by detection of the isolated GBS... Read More

A feasibility study of a leaky waveguide aptasensor for thrombin (2019)
Journal Article
Alamrani, N. A., Greenway, G. M., Pamme, N., Goddard, N. J., & Gupta, R. (in press). A feasibility study of a leaky waveguide aptasensor for thrombin. Analyst, https://doi.org/10.1039/c9an01421g

This proof-of-principle study demonstrates the feasibility of a leaky waveguide (LW) aptasensor, where aptamers were immobilised in a mesoporous chitosan waveguiding film for the detection of thrombin. This work has demonstrated that aptamers immobil... Read More

“Learning on a chip:” Microfluidics for formal and informal science education (2019)
Journal Article
Rackus, D. G., Riedel-Kruse, I. H., & Pamme, N. (2019). “Learning on a chip:” Microfluidics for formal and informal science education. Biomicrofluidics, 13(4), https://doi.org/10.1063/1.5096030

© 2019 Author(s). Microfluidics is a technique for the handling of small volumes of liquids on the order of picoliters to nanoliters and has impact for miniaturized biomedical science and fundamental research. Because of its multi- and interdisciplin... Read More

Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals (2018)
Journal Article
Tarn, M. D., Kızılyer, N. Y., Esfahani, M. M. N., Joshi, P., Brown, N. J., Pamme, N., …Archibald, S. J. (2018). Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals. Chemistry : a European journal, 24(52), 13749-13753. https://doi.org/10.1002/chem.201802395

A miniaturized radio-HPLC detector has been developed comprising a microfluidic device fabricated from plastic scintillator in combination with a silicon photomultiplier light sensor, and tested with samples containing a positron-emitting radionuclid... Read More

Fabrication of tailorable pH responsive cationic amphiphilic microgels on a microfluidic device for drug release (2017)
Journal Article
Lu, B., Tarn, M. D., Pamme, N., & Georgiou, T. K. (2018). Fabrication of tailorable pH responsive cationic amphiphilic microgels on a microfluidic device for drug release. Journal of Polymer Science Part A: Polymer Chemistry, 56(1), 59-66. https://doi.org/10.1002/pola.28860

Cationic, amphiphilic microgels of differing compositions based on hydrophilic, pH, and thermoresponsive 2-(dimethylamino)ethyl methacrylate (DMAEMA) and hydrophobic, nonionic n-butyl acrylate (BuA) are synthesized using a lab-on-a-chip device. Hydro... Read More

A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis (2017)
Journal Article
Ngamsom, B., Truyts, A., Fourie, L., Kumar, S., Tarn, M. D., Iles, A., …Pamme, N. (2017). A microfluidic device for rapid screening of E. coli O157:H7 based on IFAST and ATP bioluminescence assay for water analysis. Chemistry : a European journal, 23(52), 12754-12757. https://doi.org/10.1002/chem.201703487

© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim We present a simple microfluidic system for rapid screening of Escherichia coli (E. coli) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by s... Read More

Lab-on-a-chip workshop activities for secondary school students (2016)
Journal Article
Esfahani, M. M. N., Tarn, M. D., Choudhury, T. A., Hewitt, L. C., Mayo, A. J., Rubin, T. A., …Pamme, N. (2016). Lab-on-a-chip workshop activities for secondary school students. Biomicrofluidics, 10(1), https://doi.org/10.1063/1.4940884

The ability to engage and inspire younger generations in novel areas of science is important for bringing new researchers into a burgeoning field, such as lab-on-a-chip. We recently held a lab-on-a-chip workshop for secondary school students, for whi... Read More

Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals (2013)
Journal Article
Arima, V., Pascali, G., Lade, O., Kretschmer, H. R., Bernsdorf, I., Hammond, V., …Rinaldi, R. (2013). Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals. Lab on a chip, 13(12), 2328-2336. https://doi.org/10.1039/c3lc00055a

We have developed an integrated microfluidic platform for producing 2-[¹⁸F]-fluoro-2-deoxy-D-glucose (¹⁸F-FDG) in continuous flow from a single bolus of radioactive isotope solution, with constant product yields achieved throughout the operation that... Read More

Microfluidic platforms for performing surface-based clinical assays (2011)
Journal Article
Tarn, M. D., & Pamme, N. (2011). Microfluidic platforms for performing surface-based clinical assays. Expert review of molecular diagnostics, 11(7), 711-720. https://doi.org/10.1586/ERM.11.59

The need for fast, specific and portable diagnostic systems for clinical assays has, in recent years, led to an explosion of research into microfluidic chip-based immunoassays towards rapid point-of-care analysis. Such devices exploit small dimension... Read More

On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers (2011)
Journal Article
De Leonardis, F., Pascali, G., Salvadori, P. A., Watts, P., & Pamme, N. (2011). On-chip pre-concentration and complexation of [18F]fluoride ions via regenerable anion exchange particles for radiochemical synthesis of Positron Emission Tomography tracers. Journal of Chromatography A, 1218(29), 4714-4719. https://doi.org/10.1016/j.chroma.2011.05.062

Microfluidic approaches have demonstrated a relevant impact on radiochemical reactions involving Positron Emission Tomography (PET) nuclides, due to shorter reaction times and smaller precursor quantities. However, little attention has been given to... Read More

Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device (2011)
Journal Article
Robert, D., Pamme, N., Conjeaud, H., Gazeau, F., Iles, A., & Wilhelm, C. (2011). Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device. Lab on a chip, 11(11), 1902-1910. https://doi.org/10.1039/c0lc00656d

Magnetically labelled cells are finding a wealth of applications for in vitro analysis as well as in vivo treatments. Sorting of cells into subpopulations based on their magnetite loading is an important step in such procedures. Here, we study the so... Read More

Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles (2010)
Journal Article
Vojtíšek, M., Iles, A., & Pamme, N. (2010). Rapid, multistep on-chip DNA hybridisation in continuous flow on magnetic particles. Biosensors & bioelectronics, 25(9), 2172-2176. https://doi.org/10.1016/j.bios.2010.01.034

DNA hybridisation is an important tool for bioanalytical research and clinical diagnostics; conventional methods, however, require long incubation times and numerous washing steps, rendering the procedure time consuming and labour intensive. In this... Read More

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow (2009)
Journal Article
Peyman, S. A., Iles, A., & Pamme, N. (2009). Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow. Lab on a chip, 9(21), 3110-3117. https://doi.org/10.1039/b904724g

An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio)... Read More

Rapid on-chip multi-step (bio)chemical procedures in continuous flow - manoeuvring particles through co-laminar reagent streams (2008)
Journal Article
Peyman, S. A., Iles, A., & Pamme, N. (2008). Rapid on-chip multi-step (bio)chemical procedures in continuous flow - manoeuvring particles through co-laminar reagent streams. Chemical communications : Chem comm / the Royal Society of Chemistry, 1220-1222. https://doi.org/10.1039/b716532c

We introduce a novel and extremely versatile microfluidic platform in which tedious multi-step biochemical processes can be performed in continuous flow within a fraction of the time required for conventional methods.

Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles (2007)
Journal Article
Bronzeau, S., & Pamme, N. (2008). Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles. Analytica Chimica Acta, 609(1), 105-112. https://doi.org/10.1016/j.aca.2007.11.035

Magnetic particles coated with specific biomolecules are often used as solid supports for bioassays but conventional test tube based techniques are time consuming and labour intensive. An alternative is to work on magnetic particle plugs immobilised... Read More