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All Outputs (22)

Exploring hypoxic biology to improve radiotherapy outcomes (2022)
Journal Article
Li, C., Wiseman, L., Okoh, E., Lind, M., Roy, R., Beavis, A., & Monteiro dos Santos Pires, I. (2022). Exploring hypoxic biology to improve radiotherapy outcomes. Expert Reviews in Molecular Medicine, 24, Article E21. https://doi.org/10.1017/erm.2022.14

Ionising radiotherapy is a well-established, effective cancer treatment modality, whose efficacy has improved with the application of newer technological modalities. However, patient outcomes are governed and potentially limited by aspects of tumour... Read More about Exploring hypoxic biology to improve radiotherapy outcomes.

Predicting personalised and progressive adaptive dose escalation to gross tumour volume using knowledge-based planning models for inoperable advanced-stage non-small cell lung cancer patients treated with volumetric modulated arc therapy (2022)
Journal Article
Tambe, N., Pires, I. M., Moore, C., Wieczorek, A., Upadhyay, S., & Beavis, A. (2022). Predicting personalised and progressive adaptive dose escalation to gross tumour volume using knowledge-based planning models for inoperable advanced-stage non-small cell lung cancer patients treated with volumetric modulated arc therapy. Biomedical Physics and Engineering Express, 8(3), Article 035001. https://doi.org/10.1088/2057-1976/ac56eb

Objectives: Increased radiation doses could improve local control and overall survival of lung cancer patients, however, this could be challenging without exceeding organs at risk (OAR) dose constraints especially for patients with advanced-stage dis... Read More about Predicting personalised and progressive adaptive dose escalation to gross tumour volume using knowledge-based planning models for inoperable advanced-stage non-small cell lung cancer patients treated with volumetric modulated arc therapy.

Practical considerations of dissolved oxygen levels for platelet function under hypoxia (2021)
Journal Article
Kusanto, B., Gordon, A., Naylor-Adamson, L., Atkinson, L., Coupland, C., Booth, Z., …Arman, M. (2021). Practical considerations of dissolved oxygen levels for platelet function under hypoxia. International Journal of Molecular Sciences, 22(24), Article 13223. https://doi.org/10.3390/ijms222413223

Investigating human platelet function in low-oxygen environments is important in multiple settings, including hypobaric hypoxia (e.g., high altitude), sea level hypoxia-related disease, and thrombus stability. These studies often involve drawing bloo... Read More about Practical considerations of dissolved oxygen levels for platelet function under hypoxia.

Predicting personalised optimal arc parameter using knowledge-based planning model for inoperable locally advanced lung cancer patients to reduce organ at risk doses (2021)
Journal Article
Tambe, N. S., Pires, I. M., Moore, C., Wieczorek, A., Upadhyay, S., & Beavis, A. W. (2021). Predicting personalised optimal arc parameter using knowledge-based planning model for inoperable locally advanced lung cancer patients to reduce organ at risk doses. Biomedical Physics and Engineering Express, 7(6), Article 065016. https://doi.org/10.1088/2057-1976/ac2635

Objectives. Volumetric modulated arc therapy (VMAT) allows for reduction of organs at risk (OAR) volumes receiving higher doses, but increases OAR volumes receiving lower radiation doses and can subsequently increasing associated toxicity. Therefore,... Read More about Predicting personalised optimal arc parameter using knowledge-based planning model for inoperable locally advanced lung cancer patients to reduce organ at risk doses.

Validation of in-house knowledge-based planning model for predicting change in target coverage during VMAT radiotherapy to in-operable advanced-stage NSCLC patients (2021)
Journal Article
Tambe, N. S., Pires, I. M., Moore, C., Wieczorek, A., Upadhyay, S., & Beavis, A. W. (2021). Validation of in-house knowledge-based planning model for predicting change in target coverage during VMAT radiotherapy to in-operable advanced-stage NSCLC patients. Biomedical Physics and Engineering Express, 7(6), https://doi.org/10.1088/2057-1976/ac1f94

Objectives. anatomical changes are inevitable during the course of radiotherapy treatments and, if significant, can severely alter expected dose distributions and affect treatment outcome. Adaptive radiotherapy (ART) is employed to maintain the plann... Read More about Validation of in-house knowledge-based planning model for predicting change in target coverage during VMAT radiotherapy to in-operable advanced-stage NSCLC patients.

Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro (2021)
Journal Article
Collins, T., Pyne, E., Christensen, M., Iles, A., Pamme, N., & Pires, I. M. (2021). Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro. Biomicrofluidics, 15(4), Article 044103. https://doi.org/10.1063/5.0061373

The majority of cancer deaths are linked to tumor spread, or metastasis, but 3D in vitro metastasis models relevant to the tumor microenvironment (including interstitial fluid flow) remain an area of unmet need. Microfluidics allows us to introduce c... Read More about Spheroid-on-chip microfluidic technology for the evaluation of the impact of continuous flow on metastatic potential in cancer models in vitro.

PI3K inhibition as a novel therapeutic strategy for neoadjuvant chemoradiotherapy resistant oesophageal adenocarcinoma (2021)
Journal Article
Edge, S. D., Renard, I., Pyne, E., Li, C., Moody, H., Roy, R., …Pires, I. M. (2021). PI3K inhibition as a novel therapeutic strategy for neoadjuvant chemoradiotherapy resistant oesophageal adenocarcinoma. British Journal of Radiology, 94(1119), Article 20201191. https://doi.org/10.1259/bjr.20201191

OBJECTIVE: Neoadjuvant chemoradiotherapy (neo-CRT) prior to surgery is the standard of care for oesophageal adenocarcinoma (OAC) patients. Unfortunately, most patients fail to respond to treatment. MiR-187 was previously shown to be downregulated in... Read More about PI3K inhibition as a novel therapeutic strategy for neoadjuvant chemoradiotherapy resistant oesophageal adenocarcinoma.

Cytotoxic effects of Ridolfia segetum (L.) Moris phytoproducts in cancer cells (2020)
Journal Article
Beeby, E., Magalhães, M., Lemos, M. F. L., Pires, I. M., & Cabral, C. (in press). Cytotoxic effects of Ridolfia segetum (L.) Moris phytoproducts in cancer cells. Journal of Ethnopharmacology, Article 113515. https://doi.org/10.1016/j.jep.2020.113515

Ethnopharmacological relevance: The past few years have witnessed an increasing interest in essential oils (EOs) as potential therapeutic agents against a wide variety of pathologies, including cancer. EOs extracted from Ridolfia segetum (L.) Moris (... Read More about Cytotoxic effects of Ridolfia segetum (L.) Moris phytoproducts in cancer cells.

Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells (2020)
Journal Article
Beeby, E., Magalhães, M., Poças, J., Collins, T., Lemos, M., Barros, L., …Pires, I. M. (2020). Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells. Journal of Ethnopharmacology, 258, Article 112803. https://doi.org/10.1016/j.jep.2020.112803

Ethnopharmacological relevance: Despite advances in modern therapeutic strategies, cancer remains the second leading cause of death worldwide. Therefore, there is a constant need to develop more efficient anticancer targeting strategies. The anticanc... Read More about Secondary metabolites (essential oils) from sand-dune plants induce cytotoxic effects in cancer cells.

Validation of in-house knowledge-based planning model for advance-stage lung cancer patients treated using VMAT radiotherapy (2020)
Journal Article
Tambe, N., Pires, I. M., Moore, C., Cawthorne, C., & Beavis, A. (2020). Validation of in-house knowledge-based planning model for advance-stage lung cancer patients treated using VMAT radiotherapy. British Journal of Radiology, 93(1106), https://doi.org/10.1259/bjr.20190535

Objectives: Radiotherapy plan quality may vary considerably depending on planner's experience and time constraints. The variability in treatment plans can be assessed by calculating the difference between achieved and the optimal dose distribution. T... Read More about Validation of in-house knowledge-based planning model for advance-stage lung cancer patients treated using VMAT radiotherapy.

WSB-1 regulates the metastatic potential of hormone receptor negative breast cancer (2018)
Journal Article
Poujade, F., Mannion, A., Brittain, N., Theodosi, A., Beeby, E., Leszczynska, K. B., …Pires, I. M. (2018). WSB-1 regulates the metastatic potential of hormone receptor negative breast cancer. The British Journal of Cancer, 118(9), 1229-1237. https://doi.org/10.1038/s41416-018-0056-3

© 2018 Cancer Research UK. Background: Metastatic spread is responsible for the majority of cancer-associated deaths. The tumour microenvironment, including hypoxia, is a major driver of metastasis. The aim of this study was to investigate the role o... Read More about WSB-1 regulates the metastatic potential of hormone receptor negative breast cancer.

Replication stress and chromatin context link ATM activation to a role in DNA replication (2013)
Journal Article
Olcina, M. M., Foskolou, I. P., Anbalagan, S., Senra, J. M., Pires, I. M., Jiang, Y., …Hammond, E. M. (2013). Replication stress and chromatin context link ATM activation to a role in DNA replication. Molecular cell, 52(5), 758-766. https://doi.org/10.1016/j.molcel.2013.10.019

ATM-mediated signaling in response to DNA damage is a barrier to tumorigenesis. Here we asked whether replication stress could also contribute to ATM signaling. We demonstrate that, in the absence of DNA damage, ATM responds to replication stress in... Read More about Replication stress and chromatin context link ATM activation to a role in DNA replication.

Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions (2013)
Journal Article
Scrace, S., O'Neill, E., Hammond, E. M., & Pires, I. M. (2013). Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions. Methods in molecular biology, 1046, 295-306. https://doi.org/10.1007/978-1-62703-538-5_17

Investigation of the mechanisms behind the regulation of cellular motility and adhesion is key to understanding metastasis and the biology of tumor spreading. There are many technologies available for these studies, but the majority of them are eithe... Read More about Use of the xCELLigence system for real-time analysis of changes in cellular motility and adhesion in physiological conditions.

Targeting radiation-resistant hypoxic tumour cells through ATR inhibition (2012)
Journal Article
Monteiro dos Santos Pires, I., Olcina, M. M., Anbalagan, S., Pollard, J. R., Reaper, P. M., Charlton, P. A., …Hammond, E. M. (2012). Targeting radiation-resistant hypoxic tumour cells through ATR inhibition. The British Journal of Cancer, 107(2), 291-299. https://doi.org/10.1038/bjc.2012.265

Background:Most solid tumours contain regions of sub-optimal oxygen concentration (hypoxia). Hypoxic cancer cells are more resistant to radiotherapy and represent the most aggressive fraction of a tumour. It is therefore essential that strategies con... Read More about Targeting radiation-resistant hypoxic tumour cells through ATR inhibition.

Radiosensitization of renal cell carcinoma in vitro through the induction of autophagy (2012)
Journal Article
Anbalagan, S., Monteiro dos Santos Pires, I., Blick, C., Hill, M. A., Ferguson, D. J. P., Chan, D. A., & Hammond, E. M. (2012). Radiosensitization of renal cell carcinoma in vitro through the induction of autophagy. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 103(3), 388-393. https://doi.org/10.1016/j.radonc.2012.04.001

Background and purpose: For patients diagnosed with advanced renal cell carcinoma (RCC), there are few therapeutic options. Radiation therapy is predominantly used to treat metastasis and has not proven effective in the adjuvant setting for renal can... Read More about Radiosensitization of renal cell carcinoma in vitro through the induction of autophagy.

Hypoxia-driven cell motility reflects the interplay between JMY and HIF-1α (2011)
Journal Article
Coutts, A. S., Pires, I. M., Weston, L., Buffa, F. M., Milani, M., Li, J., …La Thangue, N. B. (2011). Hypoxia-driven cell motility reflects the interplay between JMY and HIF-1α. Oncogene, 30(48), 4835-4842. https://doi.org/10.1038/onc.2011.188

Junction-mediating and regulatory protein (JMY) is a novel p53 cofactor that regulates p53 activity during stress. JMY interacts with p300/CBP, which are ubiquitous transcriptional co-activators that interact with a variety of sequence-specific trans... Read More about Hypoxia-driven cell motility reflects the interplay between JMY and HIF-1α.

Contextual synthetic lethality of cancer cell kill based on the tumor microenvironment (2010)
Journal Article
Chan, N., Pires, I. M., Bencokova, Z., Coackley, C., Luoto, K. R., Bhogal, N., …Bristow, R. G. (2010). Contextual synthetic lethality of cancer cell kill based on the tumor microenvironment. Cancer Research, 70(20), 8045-8054. https://doi.org/10.1158/0008-5472.CAN-10-2352

Acute and chronic hypoxia exists within the three-dimensional microenvironment of solid tumors and drives therapy resistance, genetic instability, and metastasis. Replicating cells exposed to either severe acute hypoxia (16 hours with 0.02% O 2 ) fol... Read More about Contextual synthetic lethality of cancer cell kill based on the tumor microenvironment.

Regulation of autophagy by ATF4 in response to severe hypoxia (2010)
Journal Article
Rzymski, T., Milani, M., Pike, L., Buffa, F., Mellor, H. R., Winchester, L., …Harris, A. L. (2010). Regulation of autophagy by ATF4 in response to severe hypoxia. Oncogene, 29(31), 4424-4435. https://doi.org/10.1038/onc.2010.191

Activating transcription factor 4 (ATF4) is a transcription factor induced under severe hypoxia and a component of the PERK pathway involved in the unfolded protein response (UPR), a process that protects cells from the negative consequences of endop... Read More about Regulation of autophagy by ATF4 in response to severe hypoxia.

Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors (2010)
Journal Article
Pires, I. M., Ward, T. H., & Dive, C. (2010). Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors. British Journal of Pharmacology, 159(6), 1326-1338. https://doi.org/10.1111/j.1476-5381.2009.00607.x

Background and purpose: Checkpoint kinase 2 (CHK2) is activated by DNA damage and can contribute to p53 stabilization, modulating growth arrest and/or apoptosis. We investigated the contribution of CHK2 to oxaliplatin-mediated toxicity in a colorecta... Read More about Oxaliplatin responses in colorectal cancer cells are modulated by CHK2 kinase inhibitors.

Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability (2010)
Journal Article
Pires, I. M., Bencokova, Z., Milani, M., Folkes, L. K., Li, J., Stratford, M. R., …Hammond, E. M. (2010). Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability. Cancer Research, 70(3), 925-935. https://doi.org/10.1158/0008-5472.CAN-09-2715

Questions exist concerning the effects of acute versus chronic hypoxic conditions on DNA replication and genomic stability that may influence tumorigenesis. Severe hypoxia causes replication arrest independent of S-phase checkpoint, DNA damage respon... Read More about Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability.