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The Use of Tissue-on-Chip Technology to Focus the Search for Extracellular Vesicle miRNA Biomarkers in Thyroid Disease (2023)
Journal Article
Haigh, T., Beattie, H., Wade, M. A., England, J., Kuvshinov, D., Karsai, L., …Green, V. (2024). The Use of Tissue-on-Chip Technology to Focus the Search for Extracellular Vesicle miRNA Biomarkers in Thyroid Disease. International Journal of Molecular Sciences, 25(1), Article 71. https://doi.org/10.3390/ijms25010071

Small extracellular vesicles (sEVs) contain microRNAs (miRNAs) which have potential to act as disease-specific biomarkers. The current study uses an established method to maintain human thyroid tissue ex vivo on a tissue-on-chip device, allowing the... Read More about The Use of Tissue-on-Chip Technology to Focus the Search for Extracellular Vesicle miRNA Biomarkers in Thyroid Disease.

Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation (2023)
Journal Article
Green, V., Baldwin, L., England, J., Marshall, G., Frost, L., Moore, C., & Greenman, J. (2023). Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation. Cancers, 15(18), Article 4575. https://doi.org/10.3390/cancers15184575

Advancements in 3-Dimensional (3D) culture models for studying disease have increased significantly over the last two decades, but fully understanding how these models represent in vivo still requires further investigation. The current study investig... Read More about Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation.

Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system (2023)
Journal Article
Barry, A., Samuel, S. F., Hosni, I., Moursi, A., Feugere, L., Sennett, C. J., …Beltran-Alvarez, P. (2023). Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system. Lab on a chip, 23(11), 2664-2682. https://doi.org/10.1039/d3lc00204g

Arginine methylation is a post-translational modification that consists of the transfer of one or two methyl (CH3) groups to arginine residues in proteins. Several types of arginine methylation occur, namely monomethylation, symmetric dimethylation a... Read More about Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system.

Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device (2021)
Journal Article
Foster, H., Wade, M., England, J., Greenman, J., & Green, V. (2021). Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device. Organs-on-a-Chip, 3, Article 100011. https://doi.org/10.1016/j.ooc.2021.100011

Abstract This report demonstrates the ability of a microfluidic device to maintain human Graves' disease tissue enabling the isolation and characterisation of Graves' disease specific exosomes. Graves' disease (n = 7) and non-Graves’ disease (Hashim... Read More about Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device.

Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device (2021)
Journal Article
Riley, A., Jones, H., England, J., Kuvshinov, D., Green, V., & Greenman, J. (2021). Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device. Oncology Letters, 22(5), Article 780. https://doi.org/10.3892/ol.2021.13041

Although a large cohort of potential biomarkers for thyroid cancer aggressiveness have been tested in various formats in recent years, to the best of our knowledge, thyro‑ globulin and calcitonin remain the only two established biomarkers associated... Read More about Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device.

The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review (2020)
Journal Article
Chew, D., Green, V., Riley, A., England, R. J., & Greenman, J. (2020). The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review. Frontiers in Surgery, 7, Article 43. https://doi.org/10.3389/fsurg.2020.00043

© Copyright © 2020 Chew, Green, Riley, England and Greenman. Background: Thyroid cancer is the most common endocrine malignancy worldwide. Primary treatment with surgery and radioactive iodine is usually successful, however, there remains a small pro... Read More about The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review.

The effect of radioiodine treatment on the diseased thyroid gland (2019)
Journal Article
Riley, A. S., McKenzie, G. A. G., Green, V., Schettino, G., England, R. J. A., & Greenman, J. (2019). The effect of radioiodine treatment on the diseased thyroid gland. International Journal of Radiation Biology, 95(12), 1718-1727. https://doi.org/10.1080/09553002.2019.1665206

Purpose: Radioiodine (I131) therapy is the treatment mainstay for several benign and malignant thyroid disorders, however I131 is known to cause DNA damage and liberation of thyroidal self-antigens inducing secondary immunoreactivity. The exact mecha... Read More about The effect of radioiodine treatment on the diseased thyroid gland.

Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research (2019)
Journal Article
Soultanidis, G., Subiel, A., Renard, I., Reinhart, A. M., Green, V. L., Oelfke, U., …Cawthorne, C. J. (2019). Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research. Physics in Medicine and Biology, 64(12), Article 12NT02. https://doi.org/10.1088/1361-6560/ab215b

Significant improvements in radiotherapy are likely to come from biological rather than technical optimization, for example increasing tumour radiosensitivity via combination with targeted therapies. Such paradigms must first be evaluated in preclini... Read More about Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research.

A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens (2019)
Journal Article
Kennedy, R., Kuvshinov, D., Sdrolia, A., Kuvshinova, E., Hilton, K., Crank, S., …Greenman, J. (2019). A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens. Scientific reports, 9(1), Article 6327. https://doi.org/10.1038/s41598-019-42745-2

Development of personalised cancer models to predict response to radiation would benefit patient care; particularly in malignancies where treatment resistance is prevalent. Herein, a robust, easy to use, tumour-on-a-chip platform which maintains prec... Read More about A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens.

A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform (2019)
Journal Article
Riley, A., Green, V., Cheah, R., McKenzie, G., Karsai, L., England, J., & Greenman, J. (2019). A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform. BMC Cancer, 19(1), Article 259. https://doi.org/10.1186/s12885-019-5465-z

Background Though the management of malignancies has improved vastly in recent years, many treatment options lack the desired efficacy and fail to adequately augment patient morbidity and mortality. It is increasingly clear that patient response to... Read More about A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform.

A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform (2019)
Journal Article
Riley, A., Green, V., Cheah, R., McKenzie, G., Karsai, L., England, J., & Greenman, J. (2019). A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform. BMC Cancer, 19(1), Article 259. https://doi.org/10.1186/s12885-019-5465-z

© 2019 The Author(s). Background: Though the management of malignancies has improved vastly in recent years, many treatment options lack the desired efficacy and fail to adequately augment patient morbidity and mortality. It is increasingly clear tha... Read More about A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform.

The Role of Chemokines in Thyroid Carcinoma (2017)
Journal Article
Yapa, S., Mulla, O., Green, V., England, J., & Greenman, J. (2017). The Role of Chemokines in Thyroid Carcinoma. Thyroid : official journal of the American Thyroid Association, 27(11), 1347-1359. https://doi.org/10.1089/thy.2016.0660

© Copyright 2017, Mary Ann Liebert, Inc. 2017. The global incidence of thyroid cancer is increasing, and metastatic spread to the lymph nodes is common in papillary thyroid carcinoma. The metastatic course of thyroid carcinoma is an intricate process... Read More about The Role of Chemokines in Thyroid Carcinoma.

Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy (2017)
Journal Article
Cheah, R., Srivastava, R., Stafford, N. D., Beavis, A. W., Green, V., & Greenman, J. (2017). Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy. International Journal of Oncology, 51(4), 1227-1238. https://doi.org/10.3892/ijo.2017.4118

Radiotherapy is the standard treatment for head and neck squamous cell carcinoma (HNSCC), however, radioresistance remains a major clinical problem despite significant improvements in treatment protocols. Therapeutic outcome could potentially be impr... Read More about Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy.

Maintenance of head and neck tumor on-chip: gateway to personalized treatment? (2017)
Journal Article
Bower, R., Green, V. L., Kuvshinova, E., Kuvshinov, D., Karsai, L., Crank, S. T., …Greenman, J. (2017). Maintenance of head and neck tumor on-chip: gateway to personalized treatment?. Future Science OA, 3(2), FSO174. https://doi.org/10.4155/fsoa-2016-0089

Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/ res... Read More about Maintenance of head and neck tumor on-chip: gateway to personalized treatment?.

Microfluidics : the fur-free way towards personalised medicine in cancer therapy (2016)
Journal Article
Dawson, A., Greenman, J., Bower, R., & Green, V. (in press). Microfluidics : the fur-free way towards personalised medicine in cancer therapy. Drug target review, 3(1), 12-17

Microfluidic technology has great potential for complementing and, in some instances, replacing the use of animal models in the testing of medicines and in developing personalised treatments for cancer patients. The maintenance of tissue in an in viv... Read More about Microfluidics : the fur-free way towards personalised medicine in cancer therapy.

Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma (2015)
Journal Article
Wong, B. Y. W., Stafford, N. D., Green, V. L., & Greenman, J. (2016). Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma. Head & neck, 38(S1), E1903-E1908. https://doi.org/10.1002/hed.24346

© 2015 Wiley Periodicals, Inc.. Background The neutrophil/lymphocyte ratio (NLR) has been found to be predictive of survival outcome in a range of tumors. The purpose of this study was to investigate the prognostic value of pretreatment (NLR) in pati... Read More about Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma.

Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement (2013)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. (2013). Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement. Immunology, 140(3), 335-343. https://doi.org/10.1111/imm.12144

The presence of regulatory T (Treg) cells is thought to be an important mechanism by which head and neck squamous cell carcinoma (HNSCC) successfully evades the immune system. Using multicolour flow cytometry, the frequency and functional capacity of... Read More about Increased frequency and suppressive activity of CD127low/- regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement.

Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Michno, A., Greenman, J., & Stafford, N. D. (2012). Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results in immunology, 2, 1-6. https://doi.org/10.1016/j.rinim.2011.12.001

The aim of this study was to determine the effect of HNSCC tumour treatment on systemic Th1 and Th2 cytokine levels and investigate correlations with clinicopathological parameters. IL2, IL4, IL5, IL6, IL8, IL10, IL13, GMCSF, IFNγ and TNFα were measu... Read More about Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients.

Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients (2011)
Journal Article
Green, V. L., Irune, E., Prasai, A., Alhamarneh, O., Greenman, J., & Stafford, N. D. (2012). Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients. International Journal of Oncology, 40(3), 833-839. https://doi.org/10.3892/ijo.2011.1259

IL10, but not IL12 or T regulatory cells in the circulation of newly presenting, pre-treatment head and neck squamous cell carcinoma (HNSCC) patients, has been shown previously to be related to survival over a mean follow-up period of 15 months. Here... Read More about Serum IL10, IL12 and circulating CD4+CD25high T regulatory cells in relation to long-term clinical outcome in head and neck squamous cell carcinoma patients.

Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression (2011)
Journal Article
Korampalli, T. S., Green, V. L., Greenman, J., & Stafford, N. D. (2011). Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression. International Journal of Oncology, 39(4), 1033-1039. https://doi.org/10.3892/ijo.2011.1117

The expression of angiogenesis-related proteins was determined in laryngeal tumour tissue, associated tumour involved lymph nodes, apparently normal mucosa and control tissue and were related to tumour stage. Both laryngeal tumour tissue and associat... Read More about Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression.