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Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR (2022)
Journal Article
Saldanha, P. A., Bolanle, I. O., Palmer, T. M., Nikitenko, L. L., & Rivero, F. (2022). Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR. Cells, 11(15), Article 2315. https://doi.org/10.3390/cells11152315

The myosin light chain phosphatase target subunit 1 (MYPT1), encoded by the PPP1R12A gene, is a key component of the myosin light chain phosphatase (MLCP) protein complex. MYPT1 isoforms have been described as products of the cassette-type alternativ... Read More about Complex Transcriptional Profiles of the PPP1R12A Gene in Cells of the Circulatory System as Revealed by In Silico Analysis and Reverse Transcription PCR.

Bruton’s tyrosine kinase inhibitors impair FcγRIIA-driven platelet responses to bacteria in chronic lymphocytic leukemia (2021)
Journal Article
Naylor-Adamson, L., Chacko, A., Booth, Z., Caserta, S., Jarvis, J., Khan, S., …Arman, M. (2021). Bruton’s tyrosine kinase inhibitors impair FcγRIIA-driven platelet responses to bacteria in chronic lymphocytic leukemia. Frontiers in immunology, 12, Article 766272. https://doi.org/10.3389/fimmu.2021.766272

Bacterial infections are a major cause of morbidity and mortality in chronic lymphocytic leukemia (CLL), and infection risk increases in patients treated with the Bruton’s tyrosine kinase (Btk) inhibitor, ibrutinib. Btk and related kinases (like Tec)... Read More about Bruton’s tyrosine kinase inhibitors impair FcγRIIA-driven platelet responses to bacteria in chronic lymphocytic leukemia.

Interactome and evolutionary conservation of dictyostelid small GTPases and their direct regulators (2021)
Journal Article
Forbes, G., Schilde, C., Lawal, H., Kin, K., Du, Q., Chen, Z., …Schaap, P. (in press). Interactome and evolutionary conservation of dictyostelid small GTPases and their direct regulators. Small GTPases, https://doi.org/10.1080/21541248.2021.1984829

The GTP binding proteins known as small GTPases make up one of the largest groups of regulatory proteins and control almost all functions of living cells. Their activity is under respectively positive and negative regulation by guanine nucleotide exc... Read More about Interactome and evolutionary conservation of dictyostelid small GTPases and their direct regulators.

Inhibition of arginine methylation impairs platelet function (2021)
Journal Article
Marsden, A. J., Riley, D. R. J., Barry, A., Khalil, J. S., Guinn, B. A., Kemp, N. T., …Beltran-Alvarez, P. (in press). Inhibition of arginine methylation impairs platelet function. ACS Pharmacology & Translational Science, https://doi.org/10.1021/acsptsci.1c00135

Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups to arginine residues in proteins. PRMT inhibitors are novel, promising drugs against cancer that are currently in clinical trials, which include oral administration of... Read More about Inhibition of arginine methylation impairs platelet function.

Coronin 1 Is Required for Integrin β2 Translocation in Platelets (2020)
Journal Article
Riley, D. R. J., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (in press). Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International Journal of Molecular Sciences, 21(1), Article 356. https://doi.org/10.3390/ijms21010356

Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins i... Read More about Coronin 1 Is Required for Integrin β2 Translocation in Platelets.

Biochemical and immunocytochemical characterization of coronins in platelets (2019)
Journal Article
Riley, D. R., Khalil, J. S., Naseem, K. M., & Rivero, F. (in press). Biochemical and immunocytochemical characterization of coronins in platelets. Platelets, 1-12. https://doi.org/10.1080/09537104.2019.1696457

Results Platelets express at least four coronins Proteomics and transcriptomics studies indicate that both human and mouse platelets express Coro1, 2, 3 and 7, while other coronins are practically undetectable (Supplemental Table 1 and 2). To demonst... Read More about Biochemical and immunocytochemical characterization of coronins in platelets.

Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3 (2019)
Journal Article
Berger, M., Riley, D. R. J., Lutz, J., Khalil, J. S., Aburima, A., Naseem, K. M., & Rivero, F. (2019). Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3. Cells, 8(2), 149. https://doi.org/10.3390/cells8020149

Typical Rho GTPases, like Rac1, Cdc42 and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB su... Read More about Alterations in platelet alpha granule secretion and adhesion on collagen under flow in mice lacking the atypical Rho GTPase RhoBTB3.

The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart (2019)
Journal Article
Onwuli, D. O., Samuel, S., Sfyri, P., Welham, K., Goddard, M., Abu-Omar, Y., …Beltran-Alvarez, P. (2019). The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. International journal of cardiology, 282, 76-80. https://doi.org/10.1016/j.ijcard.2019.01.102

Background The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-tra... Read More about The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart.

Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line (2018)
Journal Article
Samuel, S. F., Marsden, A. J., Deepak, S., Rivero, F., Greenman, J., & Beltran-Alvarez, P. (2018). Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line. Proteomes, 6(4), Article 44. https://doi.org/10.3390/proteomes6040044

Glioblastomas (GBM) are the most common grade 4 brain tumours; patients have very poor prognosis with an average survival of 15 months after diagnosis. Novel research lines have begun to explore aberrant protein arginine methylation (ArgMe) as a poss... Read More about Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line.

Assaying Rho GTPase–dependent processes in Dictyostelium discoideum (2018)
Journal Article
Marinovic, M., Xiong, H., Rivero Crespo, F., & Weber, I. (2018). Assaying Rho GTPase–dependent processes in Dictyostelium discoideum. Methods in molecular biology, 1821, 371-392. https://doi.org/10.1007/978-1-4939-8612-5_25

The model organism D. discoideum is well-suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion and develo... Read More about Assaying Rho GTPase–dependent processes in Dictyostelium discoideum.

Methods to study the roles of Rho GTPases in platelet function (2018)
Journal Article
Rivero Crespo, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. Methods in molecular biology, 1821, 199-217. https://doi.org/10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8(1), Article 10804. https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation.

The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8, Article 10804. https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation.

Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid (2016)
Journal Article
Krey, J. F., Krystofiak, E. S., Dumont, R. A., Vijayakumar, S., Choi, D., Rivero, F., …Barr-Gillespie, P. G. (2016). Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. Journal of Cell Biology, 215(4), 467-482. https://doi.org/10.1083/jcb.201606036

With their essential role in inner-ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by crosslinkers of the plastin, fascin, and espin families. While mi... Read More about Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid.

RHOBTB3 (Rho-related BTB domain containing 3) (2016)
Journal Article
Cai, S., & Rivero, F. (2016). RHOBTB3 (Rho-related BTB domain containing 3). Atlas of genetics and cytogenetics in oncology and haematology, 20(12), https://doi.org/10.4267/2042/66947

RHOBTB3 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. In RHOBTB3 the GTPase doma... Read More about RHOBTB3 (Rho-related BTB domain containing 3).

RHOBTB1 (Rho-related BTB domain containing 1) (2016)
Journal Article
Cai, S., Rivero-Crespo, F., & Schenková, K. (2016). RHOBTB1 (Rho-related BTB domain containing 1). Atlas of genetics and cytogenetics in oncology and haematology, 20(10), 512-515. https://doi.org/10.4267/2042/66084

RhoBTB1 is one of the three members of the RhoBTB family. All RhoBTB proteins are characterized by a GTPase domain followed by a proline-rich region, a tandem of two BTB domains and a C-terminal putative RING finger domain. RHOBTB1 is a putative tumo... Read More about RHOBTB1 (Rho-related BTB domain containing 1).

Rho Signaling in Dictyostelium discoideum (2015)
Journal Article
Rivero Crespo, F., & Xiong, H. (2016). Rho Signaling in Dictyostelium discoideum. International review of cell and molecular biology, 322, 61-181. https://doi.org/10.1016/bs.ircmb.2015.10.004

© 2016 Elsevier Inc. Small GTPases of the Rho family are ubiquitous molecular switches involved in the regulation of most actin cytoskeleton dependent processes and many other processes not directly linked to actin. D. discoideum is a well-establishe... Read More about Rho Signaling in Dictyostelium discoideum.

Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice (2014)
Journal Article
Taylor, R., Bullen, A., Johnson, S. L., Grimm-Günter, E. M., Rivero, F., Marcotti, W., …Daudet, N. (2015). Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice. Human molecular genetics, 24(1), 37-49. https://doi.org/10.1093/hmg/ddu417

© The Author 2014. Published by Oxford University Press. All rights reserved. Hearing relies on the mechanosensory inner and outer hair cells (OHCs) of the organ of Corti, which convert mechanical deflections of their actin-rich stereociliary bundles... Read More about Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.

Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain (2014)
Journal Article
Lutz, J., Grimm-Günter, E. M. S., Joshi, P., & Rivero, F. (2014). Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain. Histochemistry and cell biology, 142(5), 511-528. https://doi.org/10.1007/s00418-014-1235-9

RhoBTB3 is an atypical member of the Rho family of small GTPases. It localizes at the Golgi apparatus and endosomes and is involved in vesicle trafficking and in targeting proteins for degradation in the proteasome. Previous studies using Northern bl... Read More about Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.