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All Outputs (36)

In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models (2023)
Journal Article
Moris, N., & Sturmey, R. (2023). In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models. Development, 150(17), Article dev202295. https://doi.org/10.1242/dev.202295

Several exciting advances have enabled the derivation of stem cell-based embryo-like models (SCBEMs). Such models allow us to interrogate previously intractable questions in developmental biology and ask hypothesis-driven fundamental questions such a... Read More about In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models.

Practical considerations of dissolved oxygen levels for platelet function under hypoxia (2021)
Journal Article
Kusanto, B., Gordon, A., Naylor-Adamson, L., Atkinson, L., Coupland, C., Booth, Z., …Arman, M. (2021). Practical considerations of dissolved oxygen levels for platelet function under hypoxia. International Journal of Molecular Sciences, 22(24), Article 13223. https://doi.org/10.3390/ijms222413223

Investigating human platelet function in low-oxygen environments is important in multiple settings, including hypobaric hypoxia (e.g., high altitude), sea level hypoxia-related disease, and thrombus stability. These studies often involve drawing bloo... Read More about Practical considerations of dissolved oxygen levels for platelet function under hypoxia.

Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy (2021)
Journal Article
Leese, H. J., McKeegan, P., & Sturmey, R. G. (2021). Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy. International Journal of Environmental Research and Public Health, 18(18), Article 9874. https://doi.org/10.3390/ijerph18189874

Amino acids are now recognised as having multiple cellular functions in addition to their traditional role as constituents of proteins. This is well-illustrated in the early mammalian embryo where amino acids are now known to be involved in intermedi... Read More about Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy.

Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon? (2021)
Journal Article
Atkinson, L., Martin, F., & Sturmey, R. G. (2021). Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon?. Human Reproduction, 36(7), 1737-1750. https://doi.org/10.1093/humrep/deab106

The prospect of ovarian rejuvenation offers the tantalising prospect of treating age-related declines in fertility or in pathological conditions such as premature ovarian failure. The concept of ovarian rejuvenation was invigorated by the indication... Read More about Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon?.

Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography (2020)
Journal Article
Nuhu, F., Gordon, A., Sturmey, R., Seymour, A. M., & Bhandari, S. (2020). Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography. Molecules, 25(18), Article 4196. https://doi.org/10.3390/molecules25184196

Background: Maintenance of the ratio of glutathione in the reduced (GSH) and oxidised (GSSG) state in cells is important in redox control, signal transduction and gene regulation, factors that are altered in many diseases. The accurate and reliable d... Read More about Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography.

Glucose concentration during equine in vitro maturation alters mitochondrial function (2020)
Journal Article
Lewis, N., Hinrichs, K., Leese, H. J., McGregor Argo, C., Brison, D. R., & Sturmey, R. G. (2020). Glucose concentration during equine in vitro maturation alters mitochondrial function. Reproduction, 160(2), 227-237. https://doi.org/10.1530/REP-20-0032

The use of in vitro embryo production in the horse is increasing in clinical and research settings, however, protocols are yet to be optimised. Notably, the two most commonly used base media for in vitro maturation (IVM) supply glucose at markedly di... Read More about Glucose concentration during equine in vitro maturation alters mitochondrial function.

Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization (2020)
Journal Article
Sturmey, R. G., & Leary, C. (2020). Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization. Molecular Human Reproduction, 26(4), 277-287. https://doi.org/10.1093/molehr/gaaa015

The pattern of metabolism by early embryos in vitro has been linked to a range of phenotypes, including viability. However, the extent to which metabolic function of embryos is modified by specific methods used during ART has yet to be fully describ... Read More about Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization.

Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid (2020)
Journal Article
Rodríguez-Alonso, B., Maillo, V., Acuña, O. S., López-Úbeda, R., Torrecillas, A., Simintiras, C. A., …Rizos, D. (2020). Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid. International Journal of Molecular Sciences, 21(5), Article 1681. https://doi.org/10.3390/ijms21051681

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the... Read More about Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid.

Good practice recommendations for the use of time-lapse technology† (2020)
Journal Article
Apter, S., Ebner, T., Freour, T., Guns, Y., Kovacic, B., Le Clef, N., …ESHRE Working group on Time-lapse technology. (2020). Good practice recommendations for the use of time-lapse technology†. Human Reproduction Open, 2020(2), Article hoaa008. https://doi.org/10.1093/hropen/hoaa008

STUDY QUESTION: What recommendations can be provided on the approach to and use of time-lapse technology (TLT) in an IVF laboratory? SUMMARY ANSWER: The present ESHRE document provides 11 recommendations on how to introduce TLT in the IVF laboratory... Read More about Good practice recommendations for the use of time-lapse technology†.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Journal Article
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D. R., & Sturmey, R. G. (2019). Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos. Scientific reports, 9(1), Article 16778. https://doi.org/10.1038/s41598-019-53066-9

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been rest... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

© 2019 Society for Reproduction and Fertility During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOECs). An in vitro model... Read More about Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo.

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART (2019)
Journal Article
Lagalla, C., Coticchio, G., Lagalla, C., Sturmey, R., Pennetta, F., & Borini, A. (2019). The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Human Reproduction Update, 25(4), 422-438. https://doi.org/10.1093/humupd/dmz008

Background: Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few... Read More about The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART.

Glucose metabolism and metabolic flexibility in blood platelets (2018)
Journal Article
Aibibula, M., Naseem, K. M., & Sturmey, R. (2018). Glucose metabolism and metabolic flexibility in blood platelets. Journal of thrombosis and haemostasis : JTH, 16(11), 2300-2314. https://doi.org/10.1111/jth.14274

The metabolic integration processes required for platelet activation are unclear. The metabolic plasticity of human platelets were investigated. Activated platelets exhibit a glycolytic phenotype while preserving mitochondrial function. Platelets can... Read More about Glucose metabolism and metabolic flexibility in blood platelets.

The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function (2018)
Journal Article
Lewis, N., Hinrichs, K., Brison, D., Sturmey, R., & McG. Argo, C. (2018). The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function. Journal of Equine Veterinary Science, 66, 181. https://doi.org/10.1016/j.jevs.2018.05.073

Both M199 and DMEM/F-12 support in vitro maturation (IVM) of equine oocytes and production of viable embryos after ICSI. However, these media differ markedly in glucose concentration: 5.6 mM (M199) vs. 17 mM (DMEM/F-12). Given the known impact of emb... Read More about The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function.

Effect of metabolic status on conceptus–maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome (2017)
Journal Article
Bauersachs, S., Simintiras, C. A., Sturmey, R. G., Krebs, S., Bick, J., Blum, H., …Forde, N. (2017). Effect of metabolic status on conceptus–maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome. Biology of Reproduction, 97(3), 413-425. https://doi.org/10.1093/biolre/iox095

The aim of this study was to test the hypothesis that the metabolic stresses associated with lactation alter the ability of the endometrium to respond appropriately to the conceptus by examining endometrial gene expression on Day 19 of pregnancy. Imm... Read More about Effect of metabolic status on conceptus–maternal interactions on day 19 in dairy cattle: II. Effects on the endometrial transcriptome.

Genistein crosses the bioartificial oviduct and alters secretion composition (2017)
Journal Article
Simintiras, C. A., & Sturmey, R. G. (2017). Genistein crosses the bioartificial oviduct and alters secretion composition. Reproductive Toxicology, 71, 63-70. https://doi.org/10.1016/j.reprotox.2017.04.010

The dietary derived isoflavone and oestrogen analogue, genistein, is known to perturb fundamental reproductive events such as implantation and embryo cleavage. However the question of whether genistein is able to traverse the oviduct epithelial monol... Read More about Genistein crosses the bioartificial oviduct and alters secretion composition.

Maternal metabolic stress may affect oviduct gatekeeper function (2017)
Journal Article
Jordaens, L., van Hoeck, V., Maillo, V., Gutiérrez-Adán, A., Marei, W., Vlaeminck, B., …Leroy, J. L. M. R. (2017). Maternal metabolic stress may affect oviduct gatekeeper function. Reproduction, 153(6), 759-773. https://doi.org/10.1530/rep-16-0569

We hypothesized that elevated non-esterified fatty acids (NEFA) modify in vitro bovine oviduct epithelial cell (BOEC) metabolism and barrier function. Hereto, BOECs were studied in a polarized system with 24h-treatments at day 9: 1) CONTROL (0µM NEFA... Read More about Maternal metabolic stress may affect oviduct gatekeeper function.

Modelling aspects of oviduct fluid formation in vitro (2016)
Journal Article
Simintiras, C. A., Arnold, G. J., Ulbrich, S. E., Leese, H. J., Sturmey, R. G., Froehlich, T., …Ulbrich, S. (2017). Modelling aspects of oviduct fluid formation in vitro. Reproduction, 153(1), 23-33. https://doi.org/10.1530/REP-15-0508

© 2017 Society for Reproduction and Fertility. Oviduct fluid is the microenvironment that supports early reproductive processes including fertilisation, embryo cleavage and genome activation. However, the composition and regulation of this critical e... Read More about Modelling aspects of oviduct fluid formation in vitro.

Sexually dimorphic gene expression in bovine conceptuses at the initiation of implantation (2016)
Journal Article
Forde, N., Maillo, V., O'Gaora, P., Simintiras, C. A., Sturmey, R. G., Ealy, A. D., …Lonergan, P. (2016). Sexually dimorphic gene expression in bovine conceptuses at the initiation of implantation. Biology of Reproduction, 95(4), Article ARTN 92. https://doi.org/10.1095/biolreprod.116.139857

In cattle, maternal recognition of pregnancy occurs on Day 16 via secretion of interferon tau (IFNT) by the conceptus. The endometrium can distinguish between embryos with different developmental competencies. In eutherian mammals, X-chromosome inact... Read More about Sexually dimorphic gene expression in bovine conceptuses at the initiation of implantation.