All Outputs (3)

Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny (2012)
Journal Article
Calaminus, S. D. J., Guitart, A., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., …Machesky, L. M. (2012). Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. https://doi.org/10.1371/journal.pone.0051361

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. However, in the present study we have emplo... Read More about Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny.

Actin on trafficking: could actin guide directed receptor transport? (2012)
Journal Article
Zech, T., Calaminus, S. D., & Machesky, L. M. (2012). Actin on trafficking: could actin guide directed receptor transport?. Cell adhesion & migration, 6(6), 476-481. https://doi.org/10.4161/cam.21373

Here, we present emerging ideas surrounding the interplay between the actin cytoskeleton and receptor transport and activation. The bulk of actin dynamics in cells is thought to contribute to architecture and mobility. Actin also contributes to traff... Read More about Actin on trafficking: could actin guide directed receptor transport?.

Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo (2012)
Journal Article
Schachtner, H., Li, A., Stevenson, D., Calaminus, S. D., Thomas, S. G., Watson, S. P., …Machesky, L. M. (2012). Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European journal of cell biology, 91(11-12), 923-929. https://doi.org/10.1016/j.ejcb.2012.04.002

We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblas... Read More about Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.