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All Outputs (48)

In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models (2023)
Journal Article
Moris, N., & Sturmey, R. (2023). In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models. Development, 150(17), Article dev202295. https://doi.org/10.1242/dev.202295

Several exciting advances have enabled the derivation of stem cell-based embryo-like models (SCBEMs). Such models allow us to interrogate previously intractable questions in developmental biology and ask hypothesis-driven fundamental questions such a... Read More about In preprints: opportunities to unravel the earliest stages of human development using stem cell-based embryo models.

The effect of platelet releasate on oocytes has a detrimental impact on early mammalian embryos (2023)
Thesis
Martin, F. (2023). The effect of platelet releasate on oocytes has a detrimental impact on early mammalian embryos. (Thesis). Hull York Medical School. Retrieved from https://hull-repository.worktribe.com/output/4430667

Background: Blood-derived products are a growing area of therapeutic treatments for a diverse range of conditions, including hair loss, scarring, muscle injury, wounds, arthritis, and symptoms related to the menopause. Platelet-rich plasma (PRP) is o... Read More about The effect of platelet releasate on oocytes has a detrimental impact on early mammalian embryos.

Practical considerations of dissolved oxygen levels for platelet function under hypoxia (2021)
Journal Article
Kusanto, B., Gordon, A., Naylor-Adamson, L., Atkinson, L., Coupland, C., Booth, Z., …Arman, M. (2021). Practical considerations of dissolved oxygen levels for platelet function under hypoxia. International Journal of Molecular Sciences, 22(24), Article 13223. https://doi.org/10.3390/ijms222413223

Investigating human platelet function in low-oxygen environments is important in multiple settings, including hypobaric hypoxia (e.g., high altitude), sea level hypoxia-related disease, and thrombus stability. These studies often involve drawing bloo... Read More about Practical considerations of dissolved oxygen levels for platelet function under hypoxia.

Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy (2021)
Journal Article
Leese, H. J., McKeegan, P., & Sturmey, R. G. (2021). Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy. International Journal of Environmental Research and Public Health, 18(18), Article 9874. https://doi.org/10.3390/ijerph18189874

Amino acids are now recognised as having multiple cellular functions in addition to their traditional role as constituents of proteins. This is well-illustrated in the early mammalian embryo where amino acids are now known to be involved in intermedi... Read More about Amino acids and the early mammalian embryo: Origin, fate, function and life‐long legacy.

Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon? (2021)
Journal Article
Atkinson, L., Martin, F., & Sturmey, R. G. (2021). Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon?. Human Reproduction, 36(7), 1737-1750. https://doi.org/10.1093/humrep/deab106

The prospect of ovarian rejuvenation offers the tantalising prospect of treating age-related declines in fertility or in pathological conditions such as premature ovarian failure. The concept of ovarian rejuvenation was invigorated by the indication... Read More about Intraovarian injection of platelet-rich plasma in assisted reproduction: too much too soon?.

Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography (2020)
Journal Article
Nuhu, F., Gordon, A., Sturmey, R., Seymour, A. M., & Bhandari, S. (2020). Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography. Molecules, 25(18), Article 4196. https://doi.org/10.3390/molecules25184196

Background: Maintenance of the ratio of glutathione in the reduced (GSH) and oxidised (GSSG) state in cells is important in redox control, signal transduction and gene regulation, factors that are altered in many diseases. The accurate and reliable d... Read More about Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography.

Glucose concentration during equine in vitro maturation alters mitochondrial function (2020)
Journal Article
Lewis, N., Hinrichs, K., Leese, H. J., McGregor Argo, C., Brison, D. R., & Sturmey, R. G. (2020). Glucose concentration during equine in vitro maturation alters mitochondrial function. Reproduction, 160(2), 227-237. https://doi.org/10.1530/REP-20-0032

The use of in vitro embryo production in the horse is increasing in clinical and research settings, however, protocols are yet to be optimised. Notably, the two most commonly used base media for in vitro maturation (IVM) supply glucose at markedly di... Read More about Glucose concentration during equine in vitro maturation alters mitochondrial function.

Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization (2020)
Journal Article
Sturmey, R. G., & Leary, C. (2020). Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization. Molecular Human Reproduction, 26(4), 277-287. https://doi.org/10.1093/molehr/gaaa015

The pattern of metabolism by early embryos in vitro has been linked to a range of phenotypes, including viability. However, the extent to which metabolic function of embryos is modified by specific methods used during ART has yet to be fully describ... Read More about Metabolic profile of in vitro derived human embryos is not affected by the mode of fertilization.

Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid (2020)
Journal Article
Rodríguez-Alonso, B., Maillo, V., Acuña, O. S., López-Úbeda, R., Torrecillas, A., Simintiras, C. A., …Rizos, D. (2020). Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid. International Journal of Molecular Sciences, 21(5), Article 1681. https://doi.org/10.3390/ijms21051681

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the... Read More about Spatial and pregnancy-related changes in the protein, amino acid, and carbohydrate composition of bovine oviduct fluid.

Good practice recommendations for the use of time-lapse technology† (2020)
Journal Article
Apter, S., Ebner, T., Freour, T., Guns, Y., Kovacic, B., Le Clef, N., …ESHRE Working group on Time-lapse technology. (2020). Good practice recommendations for the use of time-lapse technology†. Human Reproduction Open, 2020(2), Article hoaa008. https://doi.org/10.1093/hropen/hoaa008

STUDY QUESTION: What recommendations can be provided on the approach to and use of time-lapse technology (TLT) in an IVF laboratory? SUMMARY ANSWER: The present ESHRE document provides 11 recommendations on how to introduce TLT in the IVF laboratory... Read More about Good practice recommendations for the use of time-lapse technology†.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Journal Article
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D. R., & Sturmey, R. G. (2019). Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos. Scientific reports, 9(1), Article 16778. https://doi.org/10.1038/s41598-019-53066-9

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been rest... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Investigating the impact of hyperglycaemia on early embryo environment and development (2019)
Thesis
Roussi, K. (2019). Investigating the impact of hyperglycaemia on early embryo environment and development. (Thesis). Hull York Medical School, the University of Hull and the University of York. Retrieved from https://hull-repository.worktribe.com/output/4222139

The development of metabolic conditions has been associated with suboptimal nutrient provision to the embryo during pregnancy. Metabolic disorders have also been linked to subfertility; obese women produce fewer and smaller oocytes that are metabolic... Read More about Investigating the impact of hyperglycaemia on early embryo environment and development.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Working Paper
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D., & Sturmey, R. G. Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos

Background Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generall... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Novel aspects of mitochondrial biology in early embryos (2019)
Thesis
Muller, B. (2019). Novel aspects of mitochondrial biology in early embryos. (Thesis). Hull York Medical School, the University of Hull and the University of York. Retrieved from https://hull-repository.worktribe.com/output/4221758

Mitochondria play an essential role in early development through facilitating the production of energy required to undergo key physiological processes. Mitochondrial function in oocytes and embryos has been associated with viability and reproductive... Read More about Novel aspects of mitochondrial biology in early embryos.

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

© 2019 Society for Reproduction and Fertility During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOECs). An in vitro model... Read More about Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo.

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART (2019)
Journal Article
Lagalla, C., Coticchio, G., Lagalla, C., Sturmey, R., Pennetta, F., & Borini, A. (2019). The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Human Reproduction Update, 25(4), 422-438. https://doi.org/10.1093/humupd/dmz008

Background: Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few... Read More about The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART.

Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer (2019)
Book Chapter
McKeegan, P. J., & Sturmey, R. G. (2019). Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer. In G. Kovacs, A. Rutherford, & D. K. Gardner (Eds.), How to prepare the egg and embryo to maximize IVF success (295-304). Cambridge, UK: Cambridge University Press. https://doi.org/10.1017/9781316756744.025

A key role of cellular metabolism is the provision of energy, in the form of adenosine triphosphate (ATP). In the case of the embryo, energy demand is dynamic and highly regulated to facilitate developmental milestones such as fertilization, cleavage... Read More about Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer.

Glucose metabolism and metabolic flexibility in blood platelets (2018)
Journal Article
Aibibula, M., Naseem, K. M., & Sturmey, R. (2018). Glucose metabolism and metabolic flexibility in blood platelets. Journal of thrombosis and haemostasis : JTH, 16(11), 2300-2314. https://doi.org/10.1111/jth.14274

The metabolic integration processes required for platelet activation are unclear. The metabolic plasticity of human platelets were investigated. Activated platelets exhibit a glycolytic phenotype while preserving mitochondrial function. Platelets can... Read More about Glucose metabolism and metabolic flexibility in blood platelets.

The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function (2018)
Journal Article
Lewis, N., Hinrichs, K., Brison, D., Sturmey, R., & McG. Argo, C. (2018). The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function. Journal of Equine Veterinary Science, 66, 181. https://doi.org/10.1016/j.jevs.2018.05.073

Both M199 and DMEM/F-12 support in vitro maturation (IVM) of equine oocytes and production of viable embryos after ICSI. However, these media differ markedly in glucose concentration: 5.6 mM (M199) vs. 17 mM (DMEM/F-12). Given the known impact of emb... Read More about The effect of Glucose Concentration (5.6 mM vs. 17 mM) During IVM on Developmental and Metabolic Markers of Equine Cumulus-oocyte Complex Function.