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Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation (2023)
Journal Article
Green, V., Baldwin, L., England, J., Marshall, G., Frost, L., Moore, C., & Greenman, J. (2023). Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation. Cancers, 15(18), Article 4575. https://doi.org/10.3390/cancers15184575

Advancements in 3-Dimensional (3D) culture models for studying disease have increased significantly over the last two decades, but fully understanding how these models represent in vivo still requires further investigation. The current study investig... Read More about Head and Neck Squamous Cell Carcinoma Biopsies Maintained Ex Vivo on a Perfusion Device Show Gene Changes with Time and Clinically Relevant Doses of Irradiation.

Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system (2023)
Journal Article
Barry, A., Samuel, S. F., Hosni, I., Moursi, A., Feugere, L., Sennett, C. J., …Beltran-Alvarez, P. (2023). Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system. Lab on a chip, 23(11), 2664-2682. https://doi.org/10.1039/d3lc00204g

Arginine methylation is a post-translational modification that consists of the transfer of one or two methyl (CH3) groups to arginine residues in proteins. Several types of arginine methylation occur, namely monomethylation, symmetric dimethylation a... Read More about Investigating the effects of arginine methylation inhibitors on microdissected brain tumour biopsies maintained in a miniaturised perfusion system.

Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device (2021)
Journal Article
Foster, H., Wade, M., England, J., Greenman, J., & Green, V. (2021). Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device. Organs-on-a-Chip, 3, Article 100011. https://doi.org/10.1016/j.ooc.2021.100011

Abstract This report demonstrates the ability of a microfluidic device to maintain human Graves' disease tissue enabling the isolation and characterisation of Graves' disease specific exosomes. Graves' disease (n = 7) and non-Graves’ disease (Hashim... Read More about Isolation and characterisation of graves’ disease-specific extracellular vesicles from tissue maintained on a bespoke microfluidic device.

Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device (2021)
Journal Article
Riley, A., Jones, H., England, J., Kuvshinov, D., Green, V., & Greenman, J. (2021). Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device. Oncology Letters, 22(5), Article 780. https://doi.org/10.3892/ol.2021.13041

Although a large cohort of potential biomarkers for thyroid cancer aggressiveness have been tested in various formats in recent years, to the best of our knowledge, thyro‑ globulin and calcitonin remain the only two established biomarkers associated... Read More about Identification of soluble tissue‑derived biomarkers from human thyroid tissue explants maintained on a microfluidic device.

Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review (2021)
Journal Article
Jones, H., Green, V., England, J., & Greenman, J. (2021). Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review. Future Science OA, 7(7), https://doi.org/10.2144/fsoa-2021-0041

Thyroid cancer incidence and related mortality is increasing year-on-year, and although treatment for early disease with surgery and radioiodine results in a 98% 5-year survival rate, recurrence and treatment refractory disease is evident in an unacc... Read More about Current understanding of nonsurgical interventions for refractory differentiated thyroid cancer: a systematic review.

The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review (2020)
Journal Article
Chew, D., Green, V., Riley, A., England, R. J., & Greenman, J. (2020). The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review. Frontiers in Surgery, 7, Article 43. https://doi.org/10.3389/fsurg.2020.00043

© Copyright © 2020 Chew, Green, Riley, England and Greenman. Background: Thyroid cancer is the most common endocrine malignancy worldwide. Primary treatment with surgery and radioactive iodine is usually successful, however, there remains a small pro... Read More about The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review.

The effect of radioiodine treatment on the diseased thyroid gland (2019)
Journal Article
Riley, A. S., McKenzie, G. A. G., Green, V., Schettino, G., England, R. J. A., & Greenman, J. (2019). The effect of radioiodine treatment on the diseased thyroid gland. International Journal of Radiation Biology, 95(12), 1718-1727. https://doi.org/10.1080/09553002.2019.1665206

Purpose: Radioiodine (I131) therapy is the treatment mainstay for several benign and malignant thyroid disorders, however I131 is known to cause DNA damage and liberation of thyroidal self-antigens inducing secondary immunoreactivity. The exact mecha... Read More about The effect of radioiodine treatment on the diseased thyroid gland.

Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research (2019)
Journal Article
Soultanidis, G., Subiel, A., Renard, I., Reinhart, A. M., Green, V. L., Oelfke, U., …Cawthorne, C. J. (2019). Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research. Physics in Medicine and Biology, 64(12), Article 12NT02. https://doi.org/10.1088/1361-6560/ab215b

Significant improvements in radiotherapy are likely to come from biological rather than technical optimization, for example increasing tumour radiosensitivity via combination with targeted therapies. Such paradigms must first be evaluated in preclini... Read More about Development of an anatomically correct mouse phantom for dosimetry measurement in small animal radiotherapy research.

A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens (2019)
Journal Article
Kennedy, R., Kuvshinov, D., Sdrolia, A., Kuvshinova, E., Hilton, K., Crank, S., …Greenman, J. (2019). A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens. Scientific reports, 9(1), Article 6327. https://doi.org/10.1038/s41598-019-42745-2

Development of personalised cancer models to predict response to radiation would benefit patient care; particularly in malignancies where treatment resistance is prevalent. Herein, a robust, easy to use, tumour-on-a-chip platform which maintains prec... Read More about A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens.

A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform (2019)
Journal Article
Riley, A., Green, V., Cheah, R., McKenzie, G., Karsai, L., England, J., & Greenman, J. (2019). A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform. BMC Cancer, 19(1), Article 259. https://doi.org/10.1186/s12885-019-5465-z

Background Though the management of malignancies has improved vastly in recent years, many treatment options lack the desired efficacy and fail to adequately augment patient morbidity and mortality. It is increasingly clear that patient response to... Read More about A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform.

A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform (2019)
Journal Article
Riley, A., Green, V., Cheah, R., McKenzie, G., Karsai, L., England, J., & Greenman, J. (2019). A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform. BMC Cancer, 19(1), Article 259. https://doi.org/10.1186/s12885-019-5465-z

© 2019 The Author(s). Background: Though the management of malignancies has improved vastly in recent years, many treatment options lack the desired efficacy and fail to adequately augment patient morbidity and mortality. It is increasingly clear tha... Read More about A novel microfluidic device capable of maintaining functional thyroid carcinoma specimens ex vivo provides a new drug screening platform.

The Role of Chemokines in Thyroid Carcinoma (2017)
Journal Article
Yapa, S., Mulla, O., Green, V., England, J., & Greenman, J. (2017). The Role of Chemokines in Thyroid Carcinoma. Thyroid : official journal of the American Thyroid Association, 27(11), 1347-1359. https://doi.org/10.1089/thy.2016.0660

© Copyright 2017, Mary Ann Liebert, Inc. 2017. The global incidence of thyroid cancer is increasing, and metastatic spread to the lymph nodes is common in papillary thyroid carcinoma. The metastatic course of thyroid carcinoma is an intricate process... Read More about The Role of Chemokines in Thyroid Carcinoma.

Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy (2017)
Journal Article
Cheah, R., Srivastava, R., Stafford, N. D., Beavis, A. W., Green, V., & Greenman, J. (2017). Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy. International Journal of Oncology, 51(4), 1227-1238. https://doi.org/10.3892/ijo.2017.4118

Radiotherapy is the standard treatment for head and neck squamous cell carcinoma (HNSCC), however, radioresistance remains a major clinical problem despite significant improvements in treatment protocols. Therapeutic outcome could potentially be impr... Read More about Measuring the response of human head and neck squamous cell carcinoma to irradiation in a microfluidic model allowing customized therapy.

Maintenance of head and neck tumor on-chip: gateway to personalized treatment? (2017)
Journal Article
Bower, R., Green, V. L., Kuvshinova, E., Kuvshinov, D., Karsai, L., Crank, S. T., …Greenman, J. (2017). Maintenance of head and neck tumor on-chip: gateway to personalized treatment?. Future Science OA, 3(2), FSO174. https://doi.org/10.4155/fsoa-2016-0089

Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/ res... Read More about Maintenance of head and neck tumor on-chip: gateway to personalized treatment?.

Microfluidics : the fur-free way towards personalised medicine in cancer therapy (2016)
Journal Article
Dawson, A., Greenman, J., Bower, R., & Green, V. (in press). Microfluidics : the fur-free way towards personalised medicine in cancer therapy. Drug target review, 3(1), 12-17

Microfluidic technology has great potential for complementing and, in some instances, replacing the use of animal models in the testing of medicines and in developing personalised treatments for cancer patients. The maintenance of tissue in an in viv... Read More about Microfluidics : the fur-free way towards personalised medicine in cancer therapy.

Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine (2016)
Conference Proceeding
Bower, R., Green, V., Kuvshinov, D., Crank, S., Stafford, N., & Greenman, J. (2016). Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine.

The current paper details a succinct characterisation of an in-house designed microfluidic culture device. The maintenance of tissue viability was determined by studying single cells, following dissociation, of head and neck tumour pre and post cultu... Read More about Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine.

Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma (2015)
Journal Article
Wong, B. Y. W., Stafford, N. D., Green, V. L., & Greenman, J. (2016). Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma. Head & neck, 38(S1), E1903-E1908. https://doi.org/10.1002/hed.24346

© 2015 Wiley Periodicals, Inc.. Background The neutrophil/lymphocyte ratio (NLR) has been found to be predictive of survival outcome in a range of tumors. The purpose of this study was to investigate the prognostic value of pretreatment (NLR) in pati... Read More about Prognostic value of the neutrophil-to-lymphocyte ratio in patients with laryngeal squamous cell carcinoma.

Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients (2014)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. L. (2014). Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients. Journal of clinical & cellular immunology, 5(4), Article 241. https://doi.org/10.4172/2155-9899.1000241

Objective: Regulatory T cells (Tregs) are known to infiltrate the tumour microenvironment of many cancers, including head and neck malignancies, and are thought to contribute to the host's impaired anti-tumour immune response. However, their immunosu... Read More about Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients.

Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices (2013)
Journal Article
Green, V. L., Greenman, J., Carr, S. D., & Stafford, N. D. (2014). Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices. Otolaryngology and head and neck surgery, 150(1), 73-80. https://doi.org/10.1177/0194599813507427

Objective The aim of this study was to investigate how head and neck squamous cell carcinoma (HNSCC) tissue biopsies maintained in a pseudo in vivo environment within a bespoke microfluidic device respond to radiation treatment. Study Design Feasibi... Read More about Analysis of radiation-induced cell death in head and neck squamous cell carcinoma and rat liver maintained in microfluidic devices.