A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens (2019)
Journal Article
Kennedy, R., Kuvshinov, D., Sdrolia, A., Kuvshinova, E., Hilton, K., Crank, S., …Greenman, J. (2019). A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens. Scientific reports, 9(1), Article 6327. https://doi.org/10.1038/s41598-019-42745-2

Development of personalised cancer models to predict response to radiation would benefit patient care; particularly in malignancies where treatment resistance is prevalent. Herein, a robust, easy to use, tumour-on-a-chip platform which maintains prec... Read More about A patient tumour-on-a-chip system for personalised investigation of radiotherapy based treatment regimens.

The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart (2019)
Journal Article
Onwuli, D. O., Samuel, S., Sfyri, P., Welham, K., Goddard, M., Abu-Omar, Y., …Beltran-Alvarez, P. (2019). The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. International journal of cardiology, 282, 76-80. https://doi.org/10.1016/j.ijcard.2019.01.102

Background The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-tra... Read More about The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart.

Maintenance of head and neck tumor on-chip: gateway to personalized treatment? (2017)
Journal Article
Bower, R., Green, V. L., Kuvshinova, E., Kuvshinov, D., Karsai, L., Crank, S. T., …Greenman, J. (2017). Maintenance of head and neck tumor on-chip: gateway to personalized treatment?. Future Science OA, 3(2), FSO174. https://doi.org/10.4155/fsoa-2016-0089

Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/ res... Read More about Maintenance of head and neck tumor on-chip: gateway to personalized treatment?.

Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity (2017)
Journal Article
Spagnul, C., Turner, L. C., Giuntini, F., Greenman, J., & Boyle, R. W. (2017). Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity. Journal of Materials Chemistry B, 5(9), 1834-1845. https://doi.org/10.1039/c6tb03198f

Spectroscopic and photodynamic properties of three novel polymeric hydrogels bearing porphyrins have been studied in vitro on the recombinant bioluminescent Gram-negative Escherichia coli DH5α to assess their ability to inactivate bacterial strains i... Read More about Synthesis and bactericidal properties of porphyrins immobilized in a polyacrylamide support: influence of metal complexation on photoactivity.

Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine (2016)
Conference Proceeding
Bower, R., Green, V., Kuvshinov, D., Crank, S., Stafford, N., & Greenman, J. (2016). Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine.

The current paper details a succinct characterisation of an in-house designed microfluidic culture device. The maintenance of tissue viability was determined by studying single cells, following dissociation, of head and neck tumour pre and post cultu... Read More about Microfluidic culture of head and neck tumour maintains viability, as shown by single cell analysis, providing potential for novel applications and personalised medicine.

On-chip determination of C-reactive protein using magnetic particles in continuous flow (2014)
Journal Article
Phurimsak, C., Tarn, M. D., Peyman, S. A., Greenman, J., & Pamme, N. (2014). On-chip determination of C-reactive protein using magnetic particles in continuous flow. Analytical chemistry, 86(21), 10552-10559. https://doi.org/10.1021/ac5023265

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the... Read More about On-chip determination of C-reactive protein using magnetic particles in continuous flow.

Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions (2013)
Journal Article
Pacelli, A., Greenman, J., Cawthorne, C., & Smith, G. (2014). Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions. Journal of Labelled Compounds and Radiopharmaceuticals, 57(4), 317-322. https://doi.org/10.1002/jlcr.3160

The role of cyclooxygenase (COX)-2 as a driving force in early tumourigenesis and the current interest in the combination of COX-2 inhibitors with standard therapy in clinical trials creates an urgent need to establish clinically relevant diagnostic... Read More about Imaging COX-2 expression in cancer using PET/SPECT radioligands: current status and future directions.

Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device (2012)
Journal Article
Kemp, C., Birch, C., Shaw, K. J., Nixon, G. J., Docker, P. T., Greenman, J., …Dyer, C. E. (2012). Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device. Analytical Methods, 4(7), 2141-2144. https://doi.org/10.1039/c2ay25075f

Urine is a preferred specimen for nucleic acid-based detection of sexually transmitted infections (STIs) but represents a challenge for microfluidic devices due to low analyte concentrations. We present an extraction methodology enabling rapid on-chi... Read More about Direct processing of clinically relevant large volume samples for the detection of sexually transmitted infectious agents from urine on a microfluidic device.

Voltammetric Immunoassay for the Detection of Protein Biomarkers (2012)
Journal Article
Haswell, S. J., Dou, Y., Wadhawan, J., & Greenman, J. (2012). Voltammetric Immunoassay for the Detection of Protein Biomarkers. Electroanalysis, 24(2), 264-272. https://doi.org/10.1002/elan.201100676

A strategy for a fast (ca. 20 min), specific, electrochemical immunoassay for the cardiac biomarker creatine kinase (CK) and the human cytokine interleukin 10 (IL10) has been developed in this paper. The polyaniline modified gold surface formed from... Read More about Voltammetric Immunoassay for the Detection of Protein Biomarkers.

On-chip integrated labelling, transport and detection of tumour cells (2011)
Journal Article
Woods, J., Docker, P. T., Dyer, C. E., Haswell, S. J., & Greenman, J. (2011). On-chip integrated labelling, transport and detection of tumour cells. ELECTROPHORESIS, 32(22), 3188-3195. https://doi.org/10.1002/elps.201100172

Microflow cytometry represents a promising tool for the investigation of diagnostic and prognostic cellular cancer markers, particularly if integrated within a device that allows primary cells to be freshly isolated from the solid tumour biopsies tha... Read More about On-chip integrated labelling, transport and detection of tumour cells.

Microsystems for personalized biomolecular diagnostics (2011)
Journal Article
Shaw, K. J., Birch, C., Hughes, E. M., Jakes, A. D., Greenman, J., & Haswell, S. J. (2011). Microsystems for personalized biomolecular diagnostics. Engineering in Life Sciences, 11(2), 121-132. https://doi.org/10.1002/elsc.201000175

The development of microfluidic methodology that can be used in conjunction with drug screening and biomolecular diagnostics offers a route to evidence-based personalized medical care. Ideally, all personal diagnostics are best carried out in a rapid... Read More about Microsystems for personalized biomolecular diagnostics.

Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy (2011)
Journal Article
Vince, R. V., Madden, L. A., Alonso, C. M., Savoie, H., Boyle, R. W., Todman, M., …Greenman, J. (2011). Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy. Photochemical & photobiological sciences : an international journal, 10(4), 515-522. https://doi.org/10.1039/c0pp00267d

The increasing prevalence of multi-drug resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), necessitates development of alternative modes of bacterial targeting which are not hindered by antibiotic resistance and minimise... Read More about Identification of methicillin-resistant Staphylococcus aureus-specific peptides for targeted photoantimicrobial chemotherapy.

Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device (2010)
Journal Article
Shaw, K. J., Joyce, D., Docker, P. T., Dyer, C. E., Greenway, G. M., Greenman, J., & Haswell, S. J. (2011). Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device. Lab on a chip, 11(3), 443-448. https://doi.org/10.1039/c0lc00346h

Integrated DNA extraction and amplification have been carried out in a microfluidic device using electro-osmotic pumping (EOP) for fluidic control. All the necessary reagents for performing both DNA extraction and polymerase chain reaction (PCR) ampl... Read More about Development of a real-world direct interface for integrated DNA extraction and amplification in a microfluidic device.

Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action (2010)
Journal Article
Smith, K., Malatesti, N., Cauchon, N., Hunting, D., Lecomte, R., van Lier, J. E., …Boyle, R. W. (2011). Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology, 132(2), 256-265. https://doi.org/10.1111/j.1365-2567.2010.03359.x

Two cationic porphyrins bearing an isothiocyanate group for conjugation to monocolonal antibodies have been synthesized. The two porphyrins conjugated efficiently to three monoclonal antibodies (anti-CD104, anti-CD146 and anti-CD326), which recognize... Read More about Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.

Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species (2010)
Journal Article
Cheah, L., Dou, Y. H., Seymour, A. M. L., Dyer, C. E., Haswell, S. J., Wadhawan, J. D., & Greenman, J. (2010). Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab on a chip, 10(20), 2720-2726. https://doi.org/10.1039/c004910g

A microfluidic device has been developed to maintain viable heart tissue samples in a biomimetic microenvironment. This device allows rat or human heart tissue to be studied under pseudo in vivo conditions. Effluent levels of lactate dehydrogenase an... Read More about Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species.

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling (2010)
Journal Article
Shaw, K. J., Docker, P. T., Yelland, J. V., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2010). Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling. Lab on a chip, 10(13), 1725-1728. https://doi.org/10.1039/c000357n

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 6... Read More about Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling.

Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing (2009)
Journal Article
Dou, Y., Haswell, S., Greenman, J., & Wadhawan, J. (2009). Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing. Electrochemistry communications, 11(10), 1976-1981. https://doi.org/10.1016/j.elecom.2009.08.032

The detection of hydrogen peroxide is detailed using horseradish peroxidase and anthraquinone. Both species are immobilized on a glassy carbon electrode substrate. This dual immobilization gives rise to lower detection limits compared with the situat... Read More about Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing.

Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser (2009)
Journal Article
Oakley, J. A., Robinson, S., Dyer, C. E., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser. Analytica Chimica Acta, 652(1-2), 239-244. https://doi.org/10.1016/j.aca.2009.07.063

An integrated gel supported micro-fluidic system is reported, in which PCR products can be efficiently injected into a capillary electrophoresis device. The gel supported system is designed to provide greater stability to reagents during long periods... Read More about Development of a gel-to-gel electro-kinetic pinched injection method for an integrated micro-fluidic based DNA analyser.

CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds (2009)
Journal Article
Khan, A., Nicholson, G., McRobbie, G., Greenman, J., Pannecouque, C., Daelemans, D., …Archibald, S. J. (2009). CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds. Antiviral research, 82(2), A59-A60. https://doi.org/10.1016/j.antiviral.2009.02.141

AMD3100 is a bis-azamacrocyclic compound that has been demonstrated to be a highly effective antagonist of the CXCR4 chemokine receptor. The two azamacrocyclic rings have been shown to interact with aspartate residues on the receptor via hydrogen bon... Read More about CXCR4 antagonists: a new generation of configurationally restricted bis-azamacrocyclic compounds.

The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths (2009)
Journal Article
Shaw, K. J., Thain, L., Dyer, C., Docker, P. T., Greenman, J., Greenway, G. M., & Haswell, S. J. (2009). The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths. Analytica Chimica Acta, 652(1-2), 231-233. https://doi.org/10.1016/j.aca.2009.03.038

DNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is e... Read More about The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths.