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Morphological, ecological and molecular examination of the seacucumber species along the Red Sea coast of Egypt and Gulf of Aqaba : with the investigation of the possibility of using DNA barcoding technique as a standard method for seacucumber ID

Ahmed, Mohammed Ismail

Authors

Mohammed Ismail Ahmed



Abstract

In this study the ecology, biology and classification of the sea cucumber species of the Red Sea coast of Egypt and Gulf of Aqaba were examined in order to resolve some of the long standing question on the identification and classification of sea cucumber.The introduction of the new barcoding technique as a tool for sea cucumber identification was also tested in this study in order to assess its accuracy and potential of the technique in identifying sea cucumber individuals. A total of 18 different (species) of sea cucumber were collected from the Egyptian coast and examined for both morphological and molecular characteristics. One new species of sea cucumber were identified from the Egyptian coast of the Red Sea (Actinopyga.sp.nov). Cryptic species complex were also identified for the Holothuria atra population in the Red Sea using the molecular analysis of the mitochondrial COI gene.In this study another experiment were conducted in order to identify sea cucumber species from cooked or dried materials using the molecular techniques. As well as testing the possibility of using the DNA barcoding technique in order to identify badly/long period preserved museum specimens in order to try to identify the unknown specimens in the natural history museums around the globe.The use of the molecular DNA barcoding technique proves to be a good reliable method for sea cucumber ID; the technique was capable of resolving some of the standing taxonomic problems including the Holothuria fuscogilva /Holothuria nobilis species complex and the Pearsonthuria graeffei. The results also raised some questions about the classification of the genus Bohadchia and the Actinopyga crassa species in the Red Sea.

Citation

Ahmed, M. I. (2009). Morphological, ecological and molecular examination of the seacucumber species along the Red Sea coast of Egypt and Gulf of Aqaba : with the investigation of the possibility of using DNA barcoding technique as a standard method for seacucumber ID. (Thesis). University of Hull. Retrieved from https://hull-repository.worktribe.com/output/4209275

Thesis Type Thesis
Deposit Date Aug 15, 2011
Publicly Available Date Feb 22, 2023
Keywords Biological sciences; Marine biology
Public URL https://hull-repository.worktribe.com/output/4209275
Additional Information Department of Biological Sciences, The University of Hull
Award Date Jan 1, 2009

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Copyright Statement
© 2009 Ahmed, Mohammed Ismail. All rights reserved. No part of this publication may be reproduced without the written permission of the copyright holder.




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