All Outputs (5)

Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds (2018)
Journal Article
Harper, L. R., Buxton, A. S., Rees, H. C., Bruce, K., Brys, R., Halfmaerten, D., Read, D. S., Watson, H. V., Sayer, C. D., Jones, E. P., Priestley, V., Mächler, E., Múrria, C., Garcés-Pastor, S., Medupin, C., Burgess, K., Benson, G., Boonham, N., Griffiths, R. A., Lawson Handley, L., & Hänfling, B. (2019). Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds. Hydrobiologia, 826(1), 25-41. https://doi.org/10.1007/s10750-018-3750-5

© 2018, The Author(s). Environmental DNA (eDNA) analysis is a rapid, non-invasive, cost-efficient biodiversity monitoring tool with enormous potential to inform aquatic conservation and management. Development is ongoing, with strong commercial inter... Read More about Prospects and challenges of environmental DNA (eDNA) monitoring in freshwater ponds.

Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types (2018)
Journal Article
Sellers, G. S., Di Muri, C., Gómez, A., & Hänfling, B. (2018). Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types. Metabarcoding and Metagenomics, 2, e24556. https://doi.org/10.3897/mbmg.2.24556

Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of hi... Read More about Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types.

The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding (2018)
Journal Article
Li, J., Lawson Handley, L. J., Read, D. S., & Hänfling, B. (2018). The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding. Molecular Ecology Resources, 18(5), 1102-1114. https://doi.org/10.1111/1755-0998.12899

© 2018 John Wiley & Sons Ltd. Environmental DNA (eDNA) is a promising tool for rapid and noninvasive biodiversity monitoring. eDNA density is low in environmental samples, and a capture method, such as filtration, is often required to concentrate e... Read More about The effect of filtration method on the efficiency of environmental DNA capture and quantification via metabarcoding.

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Gough, K. C., Lewis, E., Adams, I. P., Brotherton, P., Phillips, S., & Hänfling, B. (2018). Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus). Ecology and Evolution, 8(12), 6330-6341. https://doi.org/10.1002/ece3.4013

Environmental DNA (eDNA) analysis is a rapid, cost‐effective, non‐invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species‐specific survey tool for rar... Read More about Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus).

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive (2018)
Journal Article
Hering, D., Borja, A., Jones, J. I., Pont, D., Boets, P., Bouchez, A., Bruce, K., Drakare, S., Hänfling, B., Kahlert, M., Leese, F., Meissner, K., Mergen, P., Reyjol, Y., Segurado, P., Vogler, A., & Kelly, M. (2018). Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive. Water Research, 138, 192-205. https://doi.org/10.1016/j.watres.2018.03.003

Assessment of ecological status for the European Water Framework Directive (WFD) is based on “Biological Quality Elements” (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is... Read More about Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive.