All Outputs (3)

Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains (2013)
Journal Article
Uchimoto, M. L., Beasley, E., Coult, N., Omelia, E. J., World, D., & Williams, G. (2013). Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains. Forensic Science International: Genetics, 7(4), 418-421. https://doi.org/10.1016/j.fsigen.2013.04.006

Forensic RNA analysis is gathering pace with reports of messenger RNA analysis being used in case work, and with microRNA being increasingly researched. Such techniques address a fundamental issue in body fluid identification, namely increased specif... Read More about Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains.

Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles (2013)
Journal Article
van der Meer, D., Uchimoto, M. L., & Williams, G. (2013). Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles. Journal of Forensic Sciences, 58(4), 967-971. https://doi.org/10.1111/1556-4029.12160

Micro-RNAs (miRNAs) can be specifically expressed in forensically relevant body fluids such as blood or saliva. The aim of the study was to develop a simultaneous extraction and analysis protocol that allows for the acquisition of a DNA profile and t... Read More about Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles.

Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction (2013)
Journal Article
Omelia, E. J., Uchimoto, M. L., & Williams, G. (2013). Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction. Analytical Biochemistry, 435(2), 120-122. https://doi.org/10.1016/j.ab.2012.12.024

The use of mRNA for the identification of body fluids is of particular interest in forensic science, and increasing support has been demonstrated for the use of microRNA (miRNA) analysis. MiRNA is more stable than mRNA and has been shown to be differ... Read More about Quantitative PCR analysis of blood- and saliva-specific microRNA markers following solid-phase DNA extraction.