Polymeric nanofibrous substrates stimulate pluripotent stem cells to form three-dimensional multilayered patty-like spheroids in feeder-free culture and maintain their pluripotency
(2014)
Journal Article
Alamein, M. A., Wolvetang, E. J., Ovchinnikov, D. A., Stephens, S., Sanders, K., & Warnke, P. H. (2015). Polymeric nanofibrous substrates stimulate pluripotent stem cells to form three-dimensional multilayered patty-like spheroids in feeder-free culture and maintain their pluripotency. Journal of tissue engineering and regenerative medicine, 9(9), 1078-1083. https://doi.org/10.1002/term.1960
Expansion of pluripotent stem cells in defined media devoid of animal-derived feeder cells to generate multilayered three-dimensional (3D) bulk preparations or spheroids, rather than two-dimensional (2D) monolayers, is advantageous for many regenerat... Read More about Polymeric nanofibrous substrates stimulate pluripotent stem cells to form three-dimensional multilayered patty-like spheroids in feeder-free culture and maintain their pluripotency.