All Outputs (8)

Optimising species detection probability and sampling effort in lake fish eDNA surveys (2024)
Journal Article
Sellers, G. S., Jerde, C. L., Harper, L. R., Benucci, M., Muri, C. D., Li, J., Peirson, G., Walsh, K., Hatton-Ellis, T., Duncan, W., Duguid, A., Ottewell, D., Willby, N., Law, A., Bean, C. W., Winfield, I. J., Read, D. S., Handley, L. L., & Hänfling, B. (2024). Optimising species detection probability and sampling effort in lake fish eDNA surveys. Metabarcoding and Metagenomics, 8, 121–143. https://doi.org/10.3897/mbmg.8.104655

Environmental DNA (eDNA) metabarcoding is transforming biodiversity monitoring in aquatic environments. Such an approach has been developed and deployed for monitoring lake fish communities in Great Britain, where the method has repeatedly shown a co... Read More about Optimising species detection probability and sampling effort in lake fish eDNA surveys.

Using DNA metabarcoding to investigate diet and niche partitioning in the native European otter (Lutra lutra) and invasive American mink (Neovison vison) (2020)
Journal Article
Harper, L. R., Watson, H. V., Donnelly, R., Hampshire, R., Sayer, C. D., Breithaupt, T., & Hänfling, B. (2020). Using DNA metabarcoding to investigate diet and niche partitioning in the native European otter (Lutra lutra) and invasive American mink (Neovison vison). Metabarcoding and Metagenomics, 4, 113-133. https://doi.org/10.3897/MBMG.4.56087

In the UK, the native European otter (Lutra lutra) and invasive American mink (Neovison vison) have experienced concurrent declines and expansions. Currently, the otter is recovering from persecution and waterway pollution, whereas the mink is in dec... Read More about Using DNA metabarcoding to investigate diet and niche partitioning in the native European otter (Lutra lutra) and invasive American mink (Neovison vison).

Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water (2020)
Journal Article
Blackman, R. C., Ling, K. K. S., Harper, L. R., Shum, P., Hänfling, B., & Lawson-Handley, L. (2020). Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water. Ecology and Evolution, https://doi.org/10.1002/ece3.6921

The early detection of invasive non-native species (INNS) is important for informing management actions. Established monitoring methods require the collection or observation of specimens, which is unlikely at the beginning of an invasion when densiti... Read More about Targeted and passive environmental DNA approaches outperform established methods for detection of quagga mussels, Dreissena rostriformis bugensis in flowing water.

Fishing for mammals: Landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems (2020)
Journal Article
Sales, N. G., McKenzie, M. B., Drake, J., Harper, L. R., Browett, S. S., Coscia, I., Wangensteen, O. S., Baillie, C., Bryce, E., Dawson, D. A., Ochu, E., Hänfling, B., Lawson Handley, L., Mariani, S., Lambin, X., Sutherland, C., & McDevitt, A. D. (2020). Fishing for mammals: Landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems. The journal of applied ecology, 57(4), 707-716. https://doi.org/10.1111/1365-2664.13592

© 2020 British Ecological Society Environmental DNA (eDNA) metabarcoding has revolutionized biomonitoring in both marine and freshwater ecosystems. However, for semi-aquatic and terrestrial animals, the application of this technique remains relativel... Read More about Fishing for mammals: Landscape-level monitoring of terrestrial and semi-aquatic communities using eDNA from riverine systems.

Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA (2020)
Journal Article
Blackman, R. C., Benucci, M., Donnelly, R., Hänfling, B., Harper, L. R., Sellers, G. S., & Lawson-Handley, L. (2020). Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA. Management of Biological Invasions, 11(2), 218-236. https://doi.org/10.3391/mbi.2020.11.2.04

Early detection is paramount for attempts to remove invasive non-native species (INNS). Traditional methods rely on physical sampling and morphological identification, which can be problematic when species are in low densities and/or are cryptic. The... Read More about Simple, sensitive and species-specific assays for detecting quagga and zebra mussels (Dreissena rostriformis bugensis and D. polymorpha) using environmental DNA.

Generating and testing ecological hypotheses at the pondscape with environmental DNA metabarcoding: A case study on a threatened amphibian (2019)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Lewis, E., Adams, I. P., Brotherton, P., Phillips, S., & Hänfling, B. (2020). Generating and testing ecological hypotheses at the pondscape with environmental DNA metabarcoding: A case study on a threatened amphibian. Environmental DNA, 2(2), 184-199. https://doi.org/10.1002/edn3.57

Background: Environmental DNA (eDNA) metabarcoding is revolutionizing biodiversity monitoring, but has unrealized potential for ecological hypothesis generation and testing. Aims: Here, we validate this potential in a large-scale analysis of vertebra... Read More about Generating and testing ecological hypotheses at the pondscape with environmental DNA metabarcoding: A case study on a threatened amphibian.

Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals (2019)
Journal Article
Ghazali, M., Harper, L. R., Lawson Handley, L., Carpenter, A. I., Murray-Dickson, G., Di Muri, C., Macgregor, C. J., Logan, T. W., Law, A., Breithaupt, T., Read, D. S., McDevitt, A. D., & Hänfling, B. (2019). Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals. Biological Conservation, 238(October 2019), Article 108225. https://doi.org/10.1016/j.biocon.2019.108225

© 2019 Elsevier Ltd Environmental DNA (eDNA) metabarcoding can identify terrestrial taxa utilising aquatic habitats alongside aquatic communities, but terrestrial species' eDNA dynamics are understudied. We evaluated eDNA metabarcoding for monitoring... Read More about Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals.

Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding (2019)
Journal Article
Li, J., Lawson Handley, L. J., Harper, L. R., Brys, R., Watson, H. V., Di Muri, C., Zhang, X., & Hänfling, B. (2019). Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding. Environmental DNA, 1(3), 238-250. https://doi.org/10.1002/edn3.24

Background: Environmental DNA (eDNA) metabarcoding is a promising tool for rapid, non-invasive biodiversity monitoring. Aims: In this study, eDNA metabarcoding is applied to explore the spatial and temporal distribution of fish communities in two aqu... Read More about Limited dispersion and quick degradation of environmental DNA in fish ponds inferred by metabarcoding.