The role of whole genome sequencing in characterizing the mechanism of action of para-aminosalicylic acid and its resistance

Satta, Giovanni; Witney, Adam A.; Begum, Neelu; Ortiz Canseco, Julio; Boa, Andrew N.; McHugh, Timothy D.

Abstract

Para-aminosalicylic acid (PAS) remains one of the drugs of last resort for the treatment of tuberculosis, but its mechanism of action is not completely understood yet. The main aim of this project was to identify new potential mechanisms of action and resistance to PAS by performing whole genome sequencing (WGS) on PAS-resistant laboratory mutants. A new variant in the folC gene has been identified as well as some other mutations that require further studies.

Citation

Satta, G., Witney, A. A., Begum, N., Ortiz Canseco, J., Boa, A. N., & McHugh, T. D. (2020). The role of whole genome sequencing in characterizing the mechanism of action of para-aminosalicylic acid and its resistance. Antimicrobial agents and chemotherapy, 64(9), Article e00675-20. https://doi.org/10.1128/AAC.00675-20

• Journal Article Type: Article
• Acceptance Date: Jun 13, 2020
• Online Publication Date: Jun 22, 2020
• Publication Date: Aug 20, 2020
• Deposit Date: Jul 6, 2020
• Publicly Available Date: Dec 23, 2020
• Journal: Antimicrobial agents and chemotherapy
• Print ISSN: 0066-4804
• Electronic ISSN: 1098-6596
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 64
• Issue: 9
• Article Number: e00675-20.
• DOI: https://doi.org/10.1128/AAC.00675-20
• Keywords: Pharmacology (medical); Pharmacology; Infectious Diseases
• Public URL: https://hull-repository.worktribe.com/output/3536793
• Publisher URL: https://aac.asm.org/content/early/2020/06/16/AAC.00675-20