Sporopollenin as a dilution agent in artificial diets for solitary bees

Tainsh, Fiona; Woodmansey, Shannon R.; Austin, Alexander J.; Bagnall, Toby E.; Gilbert, James D.J.

Abstract

Nutritional studies often require precise control of nutrients via dilution of artificial diets with indigestible material, but such studies in bees are limited. Common diluents like cellulose typically result in total mortality of bee larvae, making quantitative studies difficult. We investigated potential alternative dietary dilution agents, sporopollenin (pollen exines) and agar. We reared Osmia bicornis larvae on pollen diluted with these substances, alongside undiluted controls. Sporopollenin neither prevented nor improved survival, suggesting it is a suitable diluent. Agar appeared marginally to increase survival and its suitability requires further research. Both substances reduced cocoon weight, and sporopollenin also prolonged development, suggesting processing costs. Determining the physiological mechanisms driving these responses requires further work. Our findings should facilitate studies involving nutritional manipulations for solitary bees.

Citation

Tainsh, F., Woodmansey, S. R., Austin, A. J., Bagnall, T. E., & Gilbert, J. D. (in press). Sporopollenin as a dilution agent in artificial diets for solitary bees. Apidologie, https://doi.org/10.1007/s13592-020-00801-1

• Journal Article Type: Article
• Acceptance Date: Aug 3, 2020
• Online Publication Date: Oct 1, 2020
• Deposit Date: Oct 2, 2020
• Publicly Available Date: Oct 5, 2020
• Journal: Apidologie
• Print ISSN: 0044-8435
• Electronic ISSN: 1297-9678
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1007/s13592-020-00801-1
• Keywords: Artificial diet; Dilution agent; Solitary bee; Nutritional ecology; Geometric framework
• Public URL: https://hull-repository.worktribe.com/output/3620870
• Publisher URL: https://link.springer.com/article/10.1007/s13592-020-00801-1